Dynamic Salmonella Enteritidis biofilms development under different flow conditions and their removal using nanoencapsulated thymol
In food industries, microbial contaminations are difficult to control due to the recurrent formation of biofilms that hinders antimicrobials penetration and efficiency. An understanding of Salmonella Enteritidis biofilms behavior under flow conditions is a key to develop efficient preventive and con...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Biofilm |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590207522000284 |
| _version_ | 1811295688015740928 |
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| author | Jina Yammine Adem Gharsallaoui Layal Karam Ali Ismail Alexandre Fadel Nour-Eddine Chihib |
| author_facet | Jina Yammine Adem Gharsallaoui Layal Karam Ali Ismail Alexandre Fadel Nour-Eddine Chihib |
| author_sort | Jina Yammine |
| collection | DOAJ |
| description | In food industries, microbial contaminations are difficult to control due to the recurrent formation of biofilms that hinders antimicrobials penetration and efficiency. An understanding of Salmonella Enteritidis biofilms behavior under flow conditions is a key to develop efficient preventive and control strategies. S. Enteritidis biofilms displayed 5.96, 6.28 and 6.80 log CFU cm−2 under 0.006 cm s−1, 0.045 cm s−1, and 0.087 cm s−1 flow velocities, respectively. Biofilms exposed to higher nutrient conditions under greater flow rates, induced significantly more biofilm biomass. To control biofilms, the disinfection efficiency of thymol (THY) was assessed under dynamic conditions by encapsulation it into two types of nanocapsules: monolayer (ML) nanocapsules prepared with a single carrier material (maltodextrin), and layer-by-layer (LBL) nanocapsules prepared by combining two carrier materials (maltodextrin and pectin). A combined mixture of ML and LBL nanocapsules at ½ their minimal inhibitory concentrations induced 99.99% eradication of biofilms developed under the highest flow conditions, after 5 h. ML nanocapsules decreased significantly bacterial counts during the first 0.5 h, while LBL nanocapsules eliminated the remaining bacterial cells and ensured a protection from bacterial contamination for up to 5 h by releasing THY in a sustained manner over time due to the thicker shell wall structure. |
| first_indexed | 2024-04-13T05:36:18Z |
| format | Article |
| id | doaj.art-fca6b604b7054916a596771a7496a500 |
| institution | Directory Open Access Journal |
| issn | 2590-2075 |
| language | English |
| last_indexed | 2024-04-13T05:36:18Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biofilm |
| spelling | doaj.art-fca6b604b7054916a596771a7496a5002022-12-22T03:00:16ZengElsevierBiofilm2590-20752022-12-014100094Dynamic Salmonella Enteritidis biofilms development under different flow conditions and their removal using nanoencapsulated thymolJina Yammine0Adem Gharsallaoui1Layal Karam2Ali Ismail3Alexandre Fadel4Nour-Eddine Chihib5Univ Lille, CNRS, INRAE, Centrale Lille, UMR 8207 – UMET – Unité Matériaux et Transformations, Lille, France; Plateforme de Recherches et d’Analyses en Sciences de l’Environnement (PRASE), Ecole Doctorale des Sciences et Technologies, Université Libanaise, Hadath, LebanonUniv Lyon, Université Claude Bernard Lyon 1, CNRS, LAGEPP UMR, 5007, Villeurbanne, FranceHuman Nutrition Department, College of Health Sciences, QU Health, Qatar University, Doha, QatarPlateforme de Recherches et d’Analyses en Sciences de l’Environnement (PRASE), Ecole Doctorale des Sciences et Technologies, Université Libanaise, Hadath, LebanonUniv Lille, CNRS, INRAE, ENSCL, Université d’Artois, FR 2638 – IMEC – Institut Michel-Eugène Chevreul, F-59000, Lille, FranceUniv Lille, CNRS, INRAE, Centrale Lille, UMR 8207 – UMET – Unité Matériaux et Transformations, Lille, France; Corresponding author. Certia Inrae, 369 rue Jules Guesde, 59650, Villeneuve d’Ascq, France.In food industries, microbial contaminations are difficult to control due to the recurrent formation of biofilms that hinders antimicrobials penetration and efficiency. An understanding of Salmonella Enteritidis biofilms behavior under flow conditions is a key to develop efficient preventive and control strategies. S. Enteritidis biofilms displayed 5.96, 6.28 and 6.80 log CFU cm−2 under 0.006 cm s−1, 0.045 cm s−1, and 0.087 cm s−1 flow velocities, respectively. Biofilms exposed to higher nutrient conditions under greater flow rates, induced significantly more biofilm biomass. To control biofilms, the disinfection efficiency of thymol (THY) was assessed under dynamic conditions by encapsulation it into two types of nanocapsules: monolayer (ML) nanocapsules prepared with a single carrier material (maltodextrin), and layer-by-layer (LBL) nanocapsules prepared by combining two carrier materials (maltodextrin and pectin). A combined mixture of ML and LBL nanocapsules at ½ their minimal inhibitory concentrations induced 99.99% eradication of biofilms developed under the highest flow conditions, after 5 h. ML nanocapsules decreased significantly bacterial counts during the first 0.5 h, while LBL nanocapsules eliminated the remaining bacterial cells and ensured a protection from bacterial contamination for up to 5 h by releasing THY in a sustained manner over time due to the thicker shell wall structure.http://www.sciencedirect.com/science/article/pii/S2590207522000284Dynamic biofilmHydrodynamic conditionsNanoencapsulationSalmonella enteritidisDisinfectionThymol |
| spellingShingle | Jina Yammine Adem Gharsallaoui Layal Karam Ali Ismail Alexandre Fadel Nour-Eddine Chihib Dynamic Salmonella Enteritidis biofilms development under different flow conditions and their removal using nanoencapsulated thymol Biofilm Dynamic biofilm Hydrodynamic conditions Nanoencapsulation Salmonella enteritidis Disinfection Thymol |
| title | Dynamic Salmonella Enteritidis biofilms development under different flow conditions and their removal using nanoencapsulated thymol |
| title_full | Dynamic Salmonella Enteritidis biofilms development under different flow conditions and their removal using nanoencapsulated thymol |
| title_fullStr | Dynamic Salmonella Enteritidis biofilms development under different flow conditions and their removal using nanoencapsulated thymol |
| title_full_unstemmed | Dynamic Salmonella Enteritidis biofilms development under different flow conditions and their removal using nanoencapsulated thymol |
| title_short | Dynamic Salmonella Enteritidis biofilms development under different flow conditions and their removal using nanoencapsulated thymol |
| title_sort | dynamic salmonella enteritidis biofilms development under different flow conditions and their removal using nanoencapsulated thymol |
| topic | Dynamic biofilm Hydrodynamic conditions Nanoencapsulation Salmonella enteritidis Disinfection Thymol |
| url | http://www.sciencedirect.com/science/article/pii/S2590207522000284 |
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