Optimisation of Xylanase–Pectinase Cocktail Production with <i>Bacillus amyloliquefaciens</i> ADI2 Using a Low-Cost Substrate via Statistical Strategy
An effective statistical tool for increasing and boosting the production of xylanase and pectinase by <i>Bacillus amyloliquefaciens</i> ADI2 during submerged fermentation (SmF) appears to be the response of surface methodology (RSM) using the central composite design (CCD). Optimum produ...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/8/3/119 |
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| author | Muhammad Hariadi Nawawi Khairul Izdihar Ismail Norazliza Sa’ad Rosfarizan Mohamad Paridah Md Tahir Ainun Zuriyati Asa’ari Wan Zuhainis Saad |
| author_facet | Muhammad Hariadi Nawawi Khairul Izdihar Ismail Norazliza Sa’ad Rosfarizan Mohamad Paridah Md Tahir Ainun Zuriyati Asa’ari Wan Zuhainis Saad |
| author_sort | Muhammad Hariadi Nawawi |
| collection | DOAJ |
| description | An effective statistical tool for increasing and boosting the production of xylanase and pectinase by <i>Bacillus amyloliquefaciens</i> ADI2 during submerged fermentation (SmF) appears to be the response of surface methodology (RSM) using the central composite design (CCD). Optimum production was achieved under fermentation conditions of a temperature of 28 °C, pH of 8.38, inoculum size of 4% (<i>w</i>/<i>v</i>) and agitation speed of 94 rpm for 48 h. The experimental responses demonstrated a near agreement with the expected responses under optimum conditions of independent variables, suggesting the model’s validity. The optimised CCD model had a 1.34-fold, 159 ± 6 U/mL greater xylanase and 5.96-fold, 205 ± 9 U/mL greater pectinase production than the one factor at a time (OFAT) approach. The production of concurrent enzymes of xylanase–pectinase resulted in a ratio of 1:1.3. |
| first_indexed | 2024-03-09T19:52:44Z |
| format | Article |
| id | doaj.art-fcaa69ac78bf4996ae868f3f80e92dab |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-09T19:52:44Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-fcaa69ac78bf4996ae868f3f80e92dab2023-11-24T01:08:45ZengMDPI AGFermentation2311-56372022-03-018311910.3390/fermentation8030119Optimisation of Xylanase–Pectinase Cocktail Production with <i>Bacillus amyloliquefaciens</i> ADI2 Using a Low-Cost Substrate via Statistical StrategyMuhammad Hariadi Nawawi0Khairul Izdihar Ismail1Norazliza Sa’ad2Rosfarizan Mohamad3Paridah Md Tahir4Ainun Zuriyati Asa’ari5Wan Zuhainis Saad6Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, MalaysiaDepartment of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, MalaysiaDepartment of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, MalaysiaDepartment of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, MalaysiaInstitute of Tropical Forests and Forestry Products, Universiti Putra Malaysia, Serdang 43400, MalaysiaInstitute of Tropical Forests and Forestry Products, Universiti Putra Malaysia, Serdang 43400, MalaysiaDepartment of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang 43400, MalaysiaAn effective statistical tool for increasing and boosting the production of xylanase and pectinase by <i>Bacillus amyloliquefaciens</i> ADI2 during submerged fermentation (SmF) appears to be the response of surface methodology (RSM) using the central composite design (CCD). Optimum production was achieved under fermentation conditions of a temperature of 28 °C, pH of 8.38, inoculum size of 4% (<i>w</i>/<i>v</i>) and agitation speed of 94 rpm for 48 h. The experimental responses demonstrated a near agreement with the expected responses under optimum conditions of independent variables, suggesting the model’s validity. The optimised CCD model had a 1.34-fold, 159 ± 6 U/mL greater xylanase and 5.96-fold, 205 ± 9 U/mL greater pectinase production than the one factor at a time (OFAT) approach. The production of concurrent enzymes of xylanase–pectinase resulted in a ratio of 1:1.3.https://www.mdpi.com/2311-5637/8/3/119RSMxylanasepectinase<i>Bacillus</i>production |
| spellingShingle | Muhammad Hariadi Nawawi Khairul Izdihar Ismail Norazliza Sa’ad Rosfarizan Mohamad Paridah Md Tahir Ainun Zuriyati Asa’ari Wan Zuhainis Saad Optimisation of Xylanase–Pectinase Cocktail Production with <i>Bacillus amyloliquefaciens</i> ADI2 Using a Low-Cost Substrate via Statistical Strategy Fermentation RSM xylanase pectinase <i>Bacillus</i> production |
| title | Optimisation of Xylanase–Pectinase Cocktail Production with <i>Bacillus amyloliquefaciens</i> ADI2 Using a Low-Cost Substrate via Statistical Strategy |
| title_full | Optimisation of Xylanase–Pectinase Cocktail Production with <i>Bacillus amyloliquefaciens</i> ADI2 Using a Low-Cost Substrate via Statistical Strategy |
| title_fullStr | Optimisation of Xylanase–Pectinase Cocktail Production with <i>Bacillus amyloliquefaciens</i> ADI2 Using a Low-Cost Substrate via Statistical Strategy |
| title_full_unstemmed | Optimisation of Xylanase–Pectinase Cocktail Production with <i>Bacillus amyloliquefaciens</i> ADI2 Using a Low-Cost Substrate via Statistical Strategy |
| title_short | Optimisation of Xylanase–Pectinase Cocktail Production with <i>Bacillus amyloliquefaciens</i> ADI2 Using a Low-Cost Substrate via Statistical Strategy |
| title_sort | optimisation of xylanase pectinase cocktail production with i bacillus amyloliquefaciens i adi2 using a low cost substrate via statistical strategy |
| topic | RSM xylanase pectinase <i>Bacillus</i> production |
| url | https://www.mdpi.com/2311-5637/8/3/119 |
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