DARS2 promotes the occurrence of lung adenocarcinoma via the ERK/c‐Myc signaling pathway
Abstract Background DARS2 expression is upregulated in lung adenocarcinoma (LUAD) which correlates with tumor patient stage and prognosis. The mechanism of DARS2 involvement in LUAD still needs to be further explored. Methods In this study, we found that DARS2 expression in LUAD tissue was significa...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-12-01
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| Series: | Thoracic Cancer |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/1759-7714.15152 |
| _version_ | 1827577922320334848 |
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| author | Tao Fang Jin Jiang Wenhao Yu Rongyang Li Hui Tian |
| author_facet | Tao Fang Jin Jiang Wenhao Yu Rongyang Li Hui Tian |
| author_sort | Tao Fang |
| collection | DOAJ |
| description | Abstract Background DARS2 expression is upregulated in lung adenocarcinoma (LUAD) which correlates with tumor patient stage and prognosis. The mechanism of DARS2 involvement in LUAD still needs to be further explored. Methods In this study, we found that DARS2 expression in LUAD tissue was significantly higher than that in normal tissue. At the same time, the Kaplan–Meier curve showed that the survival prognosis of LUAD patients with high expression of DARS2 was significantly worse than low expression of DARS2. The expression of DARS2 was detected in LUAD and adjacent normal tissues by IHC staining, histochemical scoring and a survival curve was generated. In addition, we demonstrated that the knockdown and overexpression of DARS2 significantly affected the proliferation, invasion, and migration of LUAD cells in vitro and in vivo. Finally, western blot and rescue assay were performed on LUAD cells to further explore and verify the signaling pathway. Results DARS2 expression was significantly upregulated in LUAD tissues and cell lines. What is more, the increased expression of DARS2 was closely related to proliferation, invasion and metastasis. The tumorigenic assay in nude mice further showed that the tumorigenic ability of nude mice was significantly improved with the increase in DARS2 expression. Finally, we determined that DARS2 plays its role in LUAD by targeting the ERK/c‐Myc signaling pathway. Conclusion Our data revealed the oncogenic role of DARS2 in LUAD, indicating that DARS2 may be a predictive biomarker and novel therapeutic target for LUAD. |
| first_indexed | 2024-03-08T21:34:48Z |
| format | Article |
| id | doaj.art-fcaca692e33648b59103695fc13b9e3f |
| institution | Directory Open Access Journal |
| issn | 1759-7706 1759-7714 |
| language | English |
| last_indexed | 2024-03-08T21:34:48Z |
| publishDate | 2023-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Thoracic Cancer |
| spelling | doaj.art-fcaca692e33648b59103695fc13b9e3f2023-12-21T03:47:14ZengWileyThoracic Cancer1759-77061759-77142023-12-0114363511352110.1111/1759-7714.15152DARS2 promotes the occurrence of lung adenocarcinoma via the ERK/c‐Myc signaling pathwayTao Fang0Jin Jiang1Wenhao Yu2Rongyang Li3Hui Tian4Department of Thoracic Surgery Qilu Hospital of Shandong University Jinan ChinaDepartment of Thoracic Surgery Qilu Hospital of Shandong University Jinan ChinaDepartment of Thoracic Surgery Qilu Hospital of Shandong University Jinan ChinaDepartment of Thoracic Surgery Qilu Hospital of Shandong University Jinan ChinaDepartment of Thoracic Surgery Qilu Hospital of Shandong University Jinan ChinaAbstract Background DARS2 expression is upregulated in lung adenocarcinoma (LUAD) which correlates with tumor patient stage and prognosis. The mechanism of DARS2 involvement in LUAD still needs to be further explored. Methods In this study, we found that DARS2 expression in LUAD tissue was significantly higher than that in normal tissue. At the same time, the Kaplan–Meier curve showed that the survival prognosis of LUAD patients with high expression of DARS2 was significantly worse than low expression of DARS2. The expression of DARS2 was detected in LUAD and adjacent normal tissues by IHC staining, histochemical scoring and a survival curve was generated. In addition, we demonstrated that the knockdown and overexpression of DARS2 significantly affected the proliferation, invasion, and migration of LUAD cells in vitro and in vivo. Finally, western blot and rescue assay were performed on LUAD cells to further explore and verify the signaling pathway. Results DARS2 expression was significantly upregulated in LUAD tissues and cell lines. What is more, the increased expression of DARS2 was closely related to proliferation, invasion and metastasis. The tumorigenic assay in nude mice further showed that the tumorigenic ability of nude mice was significantly improved with the increase in DARS2 expression. Finally, we determined that DARS2 plays its role in LUAD by targeting the ERK/c‐Myc signaling pathway. Conclusion Our data revealed the oncogenic role of DARS2 in LUAD, indicating that DARS2 may be a predictive biomarker and novel therapeutic target for LUAD.https://doi.org/10.1111/1759-7714.15152DARS2ERK/c‐Myc signaling pathwaylung adenocarcinoma |
| spellingShingle | Tao Fang Jin Jiang Wenhao Yu Rongyang Li Hui Tian DARS2 promotes the occurrence of lung adenocarcinoma via the ERK/c‐Myc signaling pathway Thoracic Cancer DARS2 ERK/c‐Myc signaling pathway lung adenocarcinoma |
| title | DARS2 promotes the occurrence of lung adenocarcinoma via the ERK/c‐Myc signaling pathway |
| title_full | DARS2 promotes the occurrence of lung adenocarcinoma via the ERK/c‐Myc signaling pathway |
| title_fullStr | DARS2 promotes the occurrence of lung adenocarcinoma via the ERK/c‐Myc signaling pathway |
| title_full_unstemmed | DARS2 promotes the occurrence of lung adenocarcinoma via the ERK/c‐Myc signaling pathway |
| title_short | DARS2 promotes the occurrence of lung adenocarcinoma via the ERK/c‐Myc signaling pathway |
| title_sort | dars2 promotes the occurrence of lung adenocarcinoma via the erk c myc signaling pathway |
| topic | DARS2 ERK/c‐Myc signaling pathway lung adenocarcinoma |
| url | https://doi.org/10.1111/1759-7714.15152 |
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