Effect of Combining Low Temperature Plasma, Negative Pressure Wound Therapy, and Bone Marrow Mesenchymal Stem Cells on an Acute Skin Wound Healing Mouse Model
Low-temperature plasma (LTP; 3 min/day), negative pressure wound therapy (NPWT; 4 h/day), and bone marrow mesenchymal stem cells (MSCs; 1 × 10<sup>6</sup> cells/day) were used as mono- and combination therapy in an acute excisional skin wound-healing ICR mouse model. These therapies have...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-05-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/21/10/3675 |
| _version_ | 1797567197244030976 |
|---|---|
| author | Hui Song Cui So Young Joo Yoon Soo Cho Ji Heon Park June-Bum Kim Cheong Hoon Seo |
| author_facet | Hui Song Cui So Young Joo Yoon Soo Cho Ji Heon Park June-Bum Kim Cheong Hoon Seo |
| author_sort | Hui Song Cui |
| collection | DOAJ |
| description | Low-temperature plasma (LTP; 3 min/day), negative pressure wound therapy (NPWT; 4 h/day), and bone marrow mesenchymal stem cells (MSCs; 1 × 10<sup>6</sup> cells/day) were used as mono- and combination therapy in an acute excisional skin wound-healing ICR mouse model. These therapies have been beneficial in treating wounds. We investigated the effectiveness of monotherapy with LTP, NPWT, and MSC and combination therapy with LTP + MSC, LTP + NPWT, NPWT + MSC, and LTP + NPWT + MSC on skin wounds in mice for seven consecutive days. Gene expression, protein expression, and epithelial thickness were analyzed using real time polymerase chain reaction (RT-qPCR), western blotting, and hematoxylin and eosin staining (H&E), respectively. Wound closure was also evaluated. Wound closure was significantly accelerated in monotherapy groups, whereas more accelerated in combination therapy groups. Tumor necrosis factor-α (TNF-α) expression was increased in the LTP monotherapy group but decreased in the NPWT, MSC, and combination therapy groups. Expressions of vascular endothelial growth factor (VEGF), α-smooth muscle actin (α-SMA), and type I collagen were increased in the combination therapy groups. Re-epithelialization was also considerably accelerated in combination therapy groups. Our findings suggest that combination therapy with LPT, NPWT, and MSC exert a synergistic effect on wound healing, representing a promising strategy for the treatment of acute wounds. |
| first_indexed | 2024-03-10T19:38:14Z |
| format | Article |
| id | doaj.art-fcb2542e5ce54a94b00d18049b08a695 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T19:38:14Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-fcb2542e5ce54a94b00d18049b08a6952023-11-20T01:30:45ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-05-012110367510.3390/ijms21103675Effect of Combining Low Temperature Plasma, Negative Pressure Wound Therapy, and Bone Marrow Mesenchymal Stem Cells on an Acute Skin Wound Healing Mouse ModelHui Song Cui0So Young Joo1Yoon Soo Cho2Ji Heon Park3June-Bum Kim4Cheong Hoon Seo5Burn Institute, Department of Rehabilitation Medicine, Hangang Sacred Heart Hospital, College of Medicine, Hallym University, Seoul 07247, KoreaDepartment of Rehabilitation Medicine, Hangang Sacred Heart Hospital, College of Medicine, Hallym University, Seoul 07247, KoreaDepartment of Rehabilitation Medicine, Hangang Sacred Heart Hospital, College of Medicine, Hallym University, Seoul 07247, KoreaBurn Institute, Department of Rehabilitation Medicine, Hangang Sacred Heart Hospital, College of Medicine, Hallym University, Seoul 07247, KoreaDepartment of Pediatrics, Hangang Sacred Heart Hospital, College of Medicine, Hallym University, Seoul 07247, KoreaDepartment of Rehabilitation Medicine, Hangang Sacred Heart Hospital, College of Medicine, Hallym University, Seoul 07247, KoreaLow-temperature plasma (LTP; 3 min/day), negative pressure wound therapy (NPWT; 4 h/day), and bone marrow mesenchymal stem cells (MSCs; 1 × 10<sup>6</sup> cells/day) were used as mono- and combination therapy in an acute excisional skin wound-healing ICR mouse model. These therapies have been beneficial in treating wounds. We investigated the effectiveness of monotherapy with LTP, NPWT, and MSC and combination therapy with LTP + MSC, LTP + NPWT, NPWT + MSC, and LTP + NPWT + MSC on skin wounds in mice for seven consecutive days. Gene expression, protein expression, and epithelial thickness were analyzed using real time polymerase chain reaction (RT-qPCR), western blotting, and hematoxylin and eosin staining (H&E), respectively. Wound closure was also evaluated. Wound closure was significantly accelerated in monotherapy groups, whereas more accelerated in combination therapy groups. Tumor necrosis factor-α (TNF-α) expression was increased in the LTP monotherapy group but decreased in the NPWT, MSC, and combination therapy groups. Expressions of vascular endothelial growth factor (VEGF), α-smooth muscle actin (α-SMA), and type I collagen were increased in the combination therapy groups. Re-epithelialization was also considerably accelerated in combination therapy groups. Our findings suggest that combination therapy with LPT, NPWT, and MSC exert a synergistic effect on wound healing, representing a promising strategy for the treatment of acute wounds.https://www.mdpi.com/1422-0067/21/10/3675wound healinglow-temperature plasmanegative pressure wound therapybone marrow mesenchymal stem cellre-epithelialization |
| spellingShingle | Hui Song Cui So Young Joo Yoon Soo Cho Ji Heon Park June-Bum Kim Cheong Hoon Seo Effect of Combining Low Temperature Plasma, Negative Pressure Wound Therapy, and Bone Marrow Mesenchymal Stem Cells on an Acute Skin Wound Healing Mouse Model International Journal of Molecular Sciences wound healing low-temperature plasma negative pressure wound therapy bone marrow mesenchymal stem cell re-epithelialization |
| title | Effect of Combining Low Temperature Plasma, Negative Pressure Wound Therapy, and Bone Marrow Mesenchymal Stem Cells on an Acute Skin Wound Healing Mouse Model |
| title_full | Effect of Combining Low Temperature Plasma, Negative Pressure Wound Therapy, and Bone Marrow Mesenchymal Stem Cells on an Acute Skin Wound Healing Mouse Model |
| title_fullStr | Effect of Combining Low Temperature Plasma, Negative Pressure Wound Therapy, and Bone Marrow Mesenchymal Stem Cells on an Acute Skin Wound Healing Mouse Model |
| title_full_unstemmed | Effect of Combining Low Temperature Plasma, Negative Pressure Wound Therapy, and Bone Marrow Mesenchymal Stem Cells on an Acute Skin Wound Healing Mouse Model |
| title_short | Effect of Combining Low Temperature Plasma, Negative Pressure Wound Therapy, and Bone Marrow Mesenchymal Stem Cells on an Acute Skin Wound Healing Mouse Model |
| title_sort | effect of combining low temperature plasma negative pressure wound therapy and bone marrow mesenchymal stem cells on an acute skin wound healing mouse model |
| topic | wound healing low-temperature plasma negative pressure wound therapy bone marrow mesenchymal stem cell re-epithelialization |
| url | https://www.mdpi.com/1422-0067/21/10/3675 |
| work_keys_str_mv | AT huisongcui effectofcombininglowtemperatureplasmanegativepressurewoundtherapyandbonemarrowmesenchymalstemcellsonanacuteskinwoundhealingmousemodel AT soyoungjoo effectofcombininglowtemperatureplasmanegativepressurewoundtherapyandbonemarrowmesenchymalstemcellsonanacuteskinwoundhealingmousemodel AT yoonsoocho effectofcombininglowtemperatureplasmanegativepressurewoundtherapyandbonemarrowmesenchymalstemcellsonanacuteskinwoundhealingmousemodel AT jiheonpark effectofcombininglowtemperatureplasmanegativepressurewoundtherapyandbonemarrowmesenchymalstemcellsonanacuteskinwoundhealingmousemodel AT junebumkim effectofcombininglowtemperatureplasmanegativepressurewoundtherapyandbonemarrowmesenchymalstemcellsonanacuteskinwoundhealingmousemodel AT cheonghoonseo effectofcombininglowtemperatureplasmanegativepressurewoundtherapyandbonemarrowmesenchymalstemcellsonanacuteskinwoundhealingmousemodel |