Pathogenicity Variation in Two Genomes of Cercospora Species Causing Gray Leaf Spot in Maize
The gray leaf spots caused by Cercospora spp. severely affect the yield and quality of maize. However, the evolutionary relation and pathogenicity variation between species of the Cercospora genus is largely unknown. In this study, we constructed high-quality reference genomes by nanopore sequencing...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2023-01-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-06-22-0138-R |
| _version_ | 1797945156353130496 |
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| author | Zixiang Cheng Xiangling Lv Canxing Duan Hanyong Zhu Jianjun Wang Zhennan Xu Huifei Yin Xiaohang Zhou Mingshun Li Zhuafang Hao Fenghai Li Xinhai Li Jianfeng Weng |
| author_facet | Zixiang Cheng Xiangling Lv Canxing Duan Hanyong Zhu Jianjun Wang Zhennan Xu Huifei Yin Xiaohang Zhou Mingshun Li Zhuafang Hao Fenghai Li Xinhai Li Jianfeng Weng |
| author_sort | Zixiang Cheng |
| collection | DOAJ |
| description | The gray leaf spots caused by Cercospora spp. severely affect the yield and quality of maize. However, the evolutionary relation and pathogenicity variation between species of the Cercospora genus is largely unknown. In this study, we constructed high-quality reference genomes by nanopore sequencing two Cercospora species, namely, C. zeae-maydis and C. zeina, with differing pathogenicity, collected from northeast (Liaoning [LN]) and southeast (Yunnan [YN]) China, respectively. The genome size of C. zeae-maydis-LN is 45.08 Mb, containing 10,839 annotated genes, whereas that of Cercospora zeina-YN is 42.18 Mb, containing 10,867 annotated genes, of which approximately 86.58% are common in the two species. The difference in their genome size is largely attributed to increased long terminal repeat retrotransposons of 3.8 Mb in total length in C. zeae-maydis-LN. There are 41 and 30 carbohydrate-binding gene subfamilies identified in C. zeae-maydis-LN and C. zeina-YN, respectively. A higher number of carbohydrate-binding families found in C. zeae-maydis-LN, and its unique CBM4, CBM37, and CBM66, in particular, may contribute to variation in pathogenicity between the two species, as the carbohydrate-binding genes are known to encode cell wall–degrading enzymes. Moreover, there are 114 and 107 effectors predicted, with 47 and 46 having unique potential pathogenicity in C. zeae-maydis-LN and C. zeina-YN, respectively. Of eight effectors randomly selected for pathogenic testing, five were found to inhibit cell apoptosis induced by Bcl-2-associated X. Taken together, our results provide genomic insights into variation in pathogenicity between C. zeae-maydis and C. zeina. [Graphic: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license. |
| first_indexed | 2024-04-10T20:50:49Z |
| format | Article |
| id | doaj.art-fcb2a09acb194451a0fedfcde541fb40 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-10T20:50:49Z |
| publishDate | 2023-01-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-fcb2a09acb194451a0fedfcde541fb402023-01-23T15:38:42ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062023-01-01361142510.1094/MPMI-06-22-0138-RPathogenicity Variation in Two Genomes of Cercospora Species Causing Gray Leaf Spot in MaizeZixiang Cheng0Xiangling Lv1Canxing Duan2Hanyong Zhu3Jianjun Wang4Zhennan Xu5Huifei Yin6Xiaohang Zhou7Mingshun Li8Zhuafang Hao9Fenghai Li10Xinhai Li11Jianfeng Weng12Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang, Liaoning, 110161, ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, ChinaWenshan Academy of Agricultural Sciences, Wenshan, Yunnan, 663000, ChinaCorn Research Institute, Shanxi Agricultural University, Xinzhou, Shanxi, 030600, ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang, Liaoning, 110161, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang, Liaoning, 110161, ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, ChinaCollege of Agronomy, Shenyang Agricultural University, Shenyang, Liaoning, 110161, ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, 100081, ChinaThe gray leaf spots caused by Cercospora spp. severely affect the yield and quality of maize. However, the evolutionary relation and pathogenicity variation between species of the Cercospora genus is largely unknown. In this study, we constructed high-quality reference genomes by nanopore sequencing two Cercospora species, namely, C. zeae-maydis and C. zeina, with differing pathogenicity, collected from northeast (Liaoning [LN]) and southeast (Yunnan [YN]) China, respectively. The genome size of C. zeae-maydis-LN is 45.08 Mb, containing 10,839 annotated genes, whereas that of Cercospora zeina-YN is 42.18 Mb, containing 10,867 annotated genes, of which approximately 86.58% are common in the two species. The difference in their genome size is largely attributed to increased long terminal repeat retrotransposons of 3.8 Mb in total length in C. zeae-maydis-LN. There are 41 and 30 carbohydrate-binding gene subfamilies identified in C. zeae-maydis-LN and C. zeina-YN, respectively. A higher number of carbohydrate-binding families found in C. zeae-maydis-LN, and its unique CBM4, CBM37, and CBM66, in particular, may contribute to variation in pathogenicity between the two species, as the carbohydrate-binding genes are known to encode cell wall–degrading enzymes. Moreover, there are 114 and 107 effectors predicted, with 47 and 46 having unique potential pathogenicity in C. zeae-maydis-LN and C. zeina-YN, respectively. Of eight effectors randomly selected for pathogenic testing, five were found to inhibit cell apoptosis induced by Bcl-2-associated X. Taken together, our results provide genomic insights into variation in pathogenicity between C. zeae-maydis and C. zeina. [Graphic: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.https://apsjournals.apsnet.org/doi/10.1094/MPMI-06-22-0138-RCercospora zeae-maydisCercospora zeinagray leaf spotNanopore sequencingpathogenicity |
| spellingShingle | Zixiang Cheng Xiangling Lv Canxing Duan Hanyong Zhu Jianjun Wang Zhennan Xu Huifei Yin Xiaohang Zhou Mingshun Li Zhuafang Hao Fenghai Li Xinhai Li Jianfeng Weng Pathogenicity Variation in Two Genomes of Cercospora Species Causing Gray Leaf Spot in Maize Molecular Plant-Microbe Interactions Cercospora zeae-maydis Cercospora zeina gray leaf spot Nanopore sequencing pathogenicity |
| title | Pathogenicity Variation in Two Genomes of Cercospora Species Causing Gray Leaf Spot in Maize |
| title_full | Pathogenicity Variation in Two Genomes of Cercospora Species Causing Gray Leaf Spot in Maize |
| title_fullStr | Pathogenicity Variation in Two Genomes of Cercospora Species Causing Gray Leaf Spot in Maize |
| title_full_unstemmed | Pathogenicity Variation in Two Genomes of Cercospora Species Causing Gray Leaf Spot in Maize |
| title_short | Pathogenicity Variation in Two Genomes of Cercospora Species Causing Gray Leaf Spot in Maize |
| title_sort | pathogenicity variation in two genomes of cercospora species causing gray leaf spot in maize |
| topic | Cercospora zeae-maydis Cercospora zeina gray leaf spot Nanopore sequencing pathogenicity |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-06-22-0138-R |
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