Association between HLA-A gene polymorphism and early-onset preeclampsia in Chinese pregnant women early-onset
Abstract Background Preeclampsia is an idiopathic disease during pregnancy. This study explores the correlation between HLA-A polymorphism and the onset of preeclampsia. Methods The Illumina HiSeq2500 sequencing platform was used to genotyping HLA-A allele in venous blood DNA of 50 preeclampsia preg...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-10-01
|
| Series: | BMC Pregnancy and Childbirth |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12884-020-03340-w |
| _version_ | 1828766182637830144 |
|---|---|
| author | Yuanyuan Zheng Cui Ma Xiaowei Liu Shaowen Wu Weiyuan Zhang Shenglong Zhao |
| author_facet | Yuanyuan Zheng Cui Ma Xiaowei Liu Shaowen Wu Weiyuan Zhang Shenglong Zhao |
| author_sort | Yuanyuan Zheng |
| collection | DOAJ |
| description | Abstract Background Preeclampsia is an idiopathic disease during pregnancy. This study explores the correlation between HLA-A polymorphism and the onset of preeclampsia. Methods The Illumina HiSeq2500 sequencing platform was used to genotyping HLA-A allele in venous blood DNA of 50 preeclampsia pregnant women and 48 normal pregnant women and umbilical cord blood DNA of their children of Han nationality in China. The frequencies and distributions of alleles and genotypes among the mothers and their children were compared between the two groups. The differences of frequencies and distributions of genotypes were compared between the two groups according to the mothers’ genotype compatibility. Results Twenty HLA-A alleles were detected in preeclampsia pregnant women and normal pregnant women; 21 HLA-A alleles were found in preeclampsia group fetuses and 22 HLA-A alleles in control group fetuses. There was no statistical difference in the HLA-A genes’ frequency between the two groups of pregnant women and their fetuses. When the sharing antigen was 1, the number of maternal-fetal pairs in the preeclampsia group was more than that in the control group; the difference was statistically significant (P < 0.05). The frequency of neither mother nor fetus carrying the HLA-A * 24: 02 gene in the preeclampsia group was significantly lower than that in the control group (P < 0.05). HLA-A gene homozygosity in fetuses of early-onset preeclampsia group was substantially higher than that of the control group (P = 0.0148); there is no significant difference in pregnant women’s genes homozygosity between early-onset preeclampsia group and the control group. Conclusions HLA-A * 24: 02 may be a susceptibility gene for early preeclampsia. |
| first_indexed | 2024-12-11T07:00:43Z |
| format | Article |
| id | doaj.art-fcb4b61deefa4b3fa745d3e13efdd9a8 |
| institution | Directory Open Access Journal |
| issn | 1471-2393 |
| language | English |
| last_indexed | 2024-12-11T07:00:43Z |
| publishDate | 2020-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Pregnancy and Childbirth |
| spelling | doaj.art-fcb4b61deefa4b3fa745d3e13efdd9a82022-12-22T01:16:37ZengBMCBMC Pregnancy and Childbirth1471-23932020-10-012011610.1186/s12884-020-03340-wAssociation between HLA-A gene polymorphism and early-onset preeclampsia in Chinese pregnant women early-onsetYuanyuan Zheng0Cui Ma1Xiaowei Liu2Shaowen Wu3Weiyuan Zhang4Shenglong Zhao5Department of Obsterics, Beijing Obstetrics and Gynecology Hospital, Capital Medical UniversityDepartment of Obsterics, Beijing Obstetrics and Gynecology Hospital, Capital Medical UniversityDepartment of Obsterics, Beijing Obstetrics and Gynecology Hospital, Capital Medical UniversityDepartment of Obsterics, Beijing Obstetrics and Gynecology Hospital, Capital Medical UniversityDepartment of Obsterics, Beijing Obstetrics and Gynecology Hospital, Capital Medical UniversityDepartment of Obsterics, Beijing Obstetrics and Gynecology Hospital, Capital Medical UniversityAbstract Background Preeclampsia is an idiopathic disease during pregnancy. This study explores the correlation between HLA-A polymorphism and the onset of preeclampsia. Methods The Illumina HiSeq2500 sequencing platform was used to genotyping HLA-A allele in venous blood DNA of 50 preeclampsia pregnant women and 48 normal pregnant women and umbilical cord blood DNA of their children of Han nationality in China. The frequencies and distributions of alleles and genotypes among the mothers and their children were compared between the two groups. The differences of frequencies and distributions of genotypes were compared between the two groups according to the mothers’ genotype compatibility. Results Twenty HLA-A alleles were detected in preeclampsia pregnant women and normal pregnant women; 21 HLA-A alleles were found in preeclampsia group fetuses and 22 HLA-A alleles in control group fetuses. There was no statistical difference in the HLA-A genes’ frequency between the two groups of pregnant women and their fetuses. When the sharing antigen was 1, the number of maternal-fetal pairs in the preeclampsia group was more than that in the control group; the difference was statistically significant (P < 0.05). The frequency of neither mother nor fetus carrying the HLA-A * 24: 02 gene in the preeclampsia group was significantly lower than that in the control group (P < 0.05). HLA-A gene homozygosity in fetuses of early-onset preeclampsia group was substantially higher than that of the control group (P = 0.0148); there is no significant difference in pregnant women’s genes homozygosity between early-onset preeclampsia group and the control group. Conclusions HLA-A * 24: 02 may be a susceptibility gene for early preeclampsia.http://link.springer.com/article/10.1186/s12884-020-03340-wHuman leukocyte antigenPreeclampsiaHLA-A polymorphism |
| spellingShingle | Yuanyuan Zheng Cui Ma Xiaowei Liu Shaowen Wu Weiyuan Zhang Shenglong Zhao Association between HLA-A gene polymorphism and early-onset preeclampsia in Chinese pregnant women early-onset BMC Pregnancy and Childbirth Human leukocyte antigen Preeclampsia HLA-A polymorphism |
| title | Association between HLA-A gene polymorphism and early-onset preeclampsia in Chinese pregnant women early-onset |
| title_full | Association between HLA-A gene polymorphism and early-onset preeclampsia in Chinese pregnant women early-onset |
| title_fullStr | Association between HLA-A gene polymorphism and early-onset preeclampsia in Chinese pregnant women early-onset |
| title_full_unstemmed | Association between HLA-A gene polymorphism and early-onset preeclampsia in Chinese pregnant women early-onset |
| title_short | Association between HLA-A gene polymorphism and early-onset preeclampsia in Chinese pregnant women early-onset |
| title_sort | association between hla a gene polymorphism and early onset preeclampsia in chinese pregnant women early onset |
| topic | Human leukocyte antigen Preeclampsia HLA-A polymorphism |
| url | http://link.springer.com/article/10.1186/s12884-020-03340-w |
| work_keys_str_mv | AT yuanyuanzheng associationbetweenhlaagenepolymorphismandearlyonsetpreeclampsiainchinesepregnantwomenearlyonset AT cuima associationbetweenhlaagenepolymorphismandearlyonsetpreeclampsiainchinesepregnantwomenearlyonset AT xiaoweiliu associationbetweenhlaagenepolymorphismandearlyonsetpreeclampsiainchinesepregnantwomenearlyonset AT shaowenwu associationbetweenhlaagenepolymorphismandearlyonsetpreeclampsiainchinesepregnantwomenearlyonset AT weiyuanzhang associationbetweenhlaagenepolymorphismandearlyonsetpreeclampsiainchinesepregnantwomenearlyonset AT shenglongzhao associationbetweenhlaagenepolymorphismandearlyonsetpreeclampsiainchinesepregnantwomenearlyonset |