Using Oxidative Electrodes to Enrich Novel Members in the <i>Desulfobulbaceae</i> Family from Intertidal Sediments
Members in the family of <i>Desulfobulbaceae</i> may be influential in various anaerobic microbial communities, including those in anoxic aquatic sediments and water columns, and within wastewater treatment facilities and bioelectrochemical systems (BESs) such as microbial fuel cells (MF...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-11-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/11/2329 |
| _version_ | 1797509272002625536 |
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| author | Cheng Li Clare E. Reimers Yvan Alleau |
| author_facet | Cheng Li Clare E. Reimers Yvan Alleau |
| author_sort | Cheng Li |
| collection | DOAJ |
| description | Members in the family of <i>Desulfobulbaceae</i> may be influential in various anaerobic microbial communities, including those in anoxic aquatic sediments and water columns, and within wastewater treatment facilities and bioelectrochemical systems (BESs) such as microbial fuel cells (MFCs). However, the diversity and roles of the <i>Desulfobulbaceae</i> in these communities have received little attention, and large portions of this family remain uncultured. Here we expand on findings from an earlier study (Li, Reimers, and Alleau, 2020) to more fully characterize <i>Desulfobulbaceae</i> that became prevalent in biofilms on oxidative electrodes of bioelectrochemical reactors. After incubations, DNA extraction, microbial community analyses, and microscopic examination, we found that a group of uncultured <i>Desulfobulbaceae</i> were greatly enriched on electrode surfaces. These <i>Desulfobulbaceae</i> appeared to form filaments with morphological features ascribed to cable bacteria, but the majority were taxonomically distinct from recognized cable bacteria genera. Thus, the present study provides new information about a group of <i>Desulfobulbaceae</i> that can exhibit filamentous morphologies and respire on the oxidative electrodes. While the phylogeny of cable bacteria is still being defined and updated, further enriching these members can contribute to the overall understanding of cable bacteria and may also lead to identification of successful isolation strategies. |
| first_indexed | 2024-03-10T05:15:21Z |
| format | Article |
| id | doaj.art-fcb9492417924fbdb326815ac009f1da |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T05:15:21Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-fcb9492417924fbdb326815ac009f1da2023-11-23T00:29:48ZengMDPI AGMicroorganisms2076-26072021-11-01911232910.3390/microorganisms9112329Using Oxidative Electrodes to Enrich Novel Members in the <i>Desulfobulbaceae</i> Family from Intertidal SedimentsCheng Li0Clare E. Reimers1Yvan Alleau2College of Earth, Ocean, and Atmospheric Sciences, Oregon State University, Corvallis, OR 97331, USACollege of Earth, Ocean, and Atmospheric Sciences, Oregon State University, Corvallis, OR 97331, USACollege of Earth, Ocean, and Atmospheric Sciences, Oregon State University, Corvallis, OR 97331, USAMembers in the family of <i>Desulfobulbaceae</i> may be influential in various anaerobic microbial communities, including those in anoxic aquatic sediments and water columns, and within wastewater treatment facilities and bioelectrochemical systems (BESs) such as microbial fuel cells (MFCs). However, the diversity and roles of the <i>Desulfobulbaceae</i> in these communities have received little attention, and large portions of this family remain uncultured. Here we expand on findings from an earlier study (Li, Reimers, and Alleau, 2020) to more fully characterize <i>Desulfobulbaceae</i> that became prevalent in biofilms on oxidative electrodes of bioelectrochemical reactors. After incubations, DNA extraction, microbial community analyses, and microscopic examination, we found that a group of uncultured <i>Desulfobulbaceae</i> were greatly enriched on electrode surfaces. These <i>Desulfobulbaceae</i> appeared to form filaments with morphological features ascribed to cable bacteria, but the majority were taxonomically distinct from recognized cable bacteria genera. Thus, the present study provides new information about a group of <i>Desulfobulbaceae</i> that can exhibit filamentous morphologies and respire on the oxidative electrodes. While the phylogeny of cable bacteria is still being defined and updated, further enriching these members can contribute to the overall understanding of cable bacteria and may also lead to identification of successful isolation strategies.https://www.mdpi.com/2076-2607/9/11/2329<i>Desulfobulbaceae</i>bioelectrochemical reactorelectrogenelectrogenic sulfur oxidationcable bacteria |
| spellingShingle | Cheng Li Clare E. Reimers Yvan Alleau Using Oxidative Electrodes to Enrich Novel Members in the <i>Desulfobulbaceae</i> Family from Intertidal Sediments Microorganisms <i>Desulfobulbaceae</i> bioelectrochemical reactor electrogen electrogenic sulfur oxidation cable bacteria |
| title | Using Oxidative Electrodes to Enrich Novel Members in the <i>Desulfobulbaceae</i> Family from Intertidal Sediments |
| title_full | Using Oxidative Electrodes to Enrich Novel Members in the <i>Desulfobulbaceae</i> Family from Intertidal Sediments |
| title_fullStr | Using Oxidative Electrodes to Enrich Novel Members in the <i>Desulfobulbaceae</i> Family from Intertidal Sediments |
| title_full_unstemmed | Using Oxidative Electrodes to Enrich Novel Members in the <i>Desulfobulbaceae</i> Family from Intertidal Sediments |
| title_short | Using Oxidative Electrodes to Enrich Novel Members in the <i>Desulfobulbaceae</i> Family from Intertidal Sediments |
| title_sort | using oxidative electrodes to enrich novel members in the i desulfobulbaceae i family from intertidal sediments |
| topic | <i>Desulfobulbaceae</i> bioelectrochemical reactor electrogen electrogenic sulfur oxidation cable bacteria |
| url | https://www.mdpi.com/2076-2607/9/11/2329 |
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