Development and in-vivo validation of a portable phosphorescence lifetime-based fiber-optic oxygen sensor
Abstract Oxygenation is a crucial indicator of tissue viability and function. Oxygen tension ( $$\hbox {pO}_2$$ pO 2 ), i.e. the amount of molecular oxygen present in the tissue is a direct result of supply (perfusion) and consumption. Thus, measurement of $$\hbox {pO}_{{2}}$$ pO 2 is an effective m...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-41917-5 |
| _version_ | 1797452824220532736 |
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| author | Lilian Witthauer Emmanuel Roussakis Juan Pedro Cascales Avery Goss Xiaolei Li Alexis Cralley Dor Yoeli Hunter B. Moore Zhaohui Wang Yong Wang Bing Li Christene A. Huang Ernest E. Moore Conor L. Evans |
| author_facet | Lilian Witthauer Emmanuel Roussakis Juan Pedro Cascales Avery Goss Xiaolei Li Alexis Cralley Dor Yoeli Hunter B. Moore Zhaohui Wang Yong Wang Bing Li Christene A. Huang Ernest E. Moore Conor L. Evans |
| author_sort | Lilian Witthauer |
| collection | DOAJ |
| description | Abstract Oxygenation is a crucial indicator of tissue viability and function. Oxygen tension ( $$\hbox {pO}_2$$ pO 2 ), i.e. the amount of molecular oxygen present in the tissue is a direct result of supply (perfusion) and consumption. Thus, measurement of $$\hbox {pO}_{{2}}$$ pO 2 is an effective method to monitor tissue viability. However, tissue oximetry sensors commonly used in clinical practice instead rely on measuring oxygen saturation ( $$\hbox {StO}_2$$ StO 2 ), largely due to the lack of reliable, affordable $$\hbox {pO}_2$$ pO 2 sensing solutions. To address this issue we present a proof-of-concept design and validation of a low-cost, lifetime-based oxygen sensing fiber. The sensor consists of readily-available off-the shelf components such as a microcontroller, a light-emitting diode (LED), an avalanche photodiode (APD), a temperature sensor, as well as a bright in-house developed porphyrin molecule. The device was calibrated using a benchtop setup and evaluated in three in vivo animal models. Our findings show that the new device design in combination with the bright porphyrin has the potential to be a useful and accurate tool for measuring $$\hbox {pO}_2$$ pO 2 in tissue, while also highlighting some of the limitations and challenges of oxygen measurements in this context. |
| first_indexed | 2024-03-09T15:14:15Z |
| format | Article |
| id | doaj.art-fcb9ed8b98f84019a0c8af81e4f18256 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T15:14:15Z |
| publishDate | 2023-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-fcb9ed8b98f84019a0c8af81e4f182562023-11-26T13:14:27ZengNature PortfolioScientific Reports2045-23222023-09-0113111010.1038/s41598-023-41917-5Development and in-vivo validation of a portable phosphorescence lifetime-based fiber-optic oxygen sensorLilian Witthauer0Emmanuel Roussakis1Juan Pedro Cascales2Avery Goss3Xiaolei Li4Alexis Cralley5Dor Yoeli6Hunter B. Moore7Zhaohui Wang8Yong Wang9Bing Li10Christene A. Huang11Ernest E. Moore12Conor L. Evans13Wellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical SchoolWellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical SchoolWellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical SchoolWellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical SchoolWellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical SchoolDepartment of Surgery, University of Colorado Denver/Anschutz Medical CampusDepartment of Surgery, University of Colorado Denver/Anschutz Medical CampusDepartment of Surgery, University of Colorado Denver/Anschutz Medical CampusDepartment of Surgery, University of Colorado Denver/Anschutz Medical CampusDepartment of Surgery, University of Colorado Denver/Anschutz Medical CampusDepartment of Surgery, University of Colorado Denver/Anschutz Medical CampusDepartment of Surgery, University of Colorado Denver/Anschutz Medical CampusDepartment of Surgery, University of Colorado Denver/Anschutz Medical CampusWellman Center for Photomedicine, Massachusetts General Hospital, Harvard Medical SchoolAbstract Oxygenation is a crucial indicator of tissue viability and function. Oxygen tension ( $$\hbox {pO}_2$$ pO 2 ), i.e. the amount of molecular oxygen present in the tissue is a direct result of supply (perfusion) and consumption. Thus, measurement of $$\hbox {pO}_{{2}}$$ pO 2 is an effective method to monitor tissue viability. However, tissue oximetry sensors commonly used in clinical practice instead rely on measuring oxygen saturation ( $$\hbox {StO}_2$$ StO 2 ), largely due to the lack of reliable, affordable $$\hbox {pO}_2$$ pO 2 sensing solutions. To address this issue we present a proof-of-concept design and validation of a low-cost, lifetime-based oxygen sensing fiber. The sensor consists of readily-available off-the shelf components such as a microcontroller, a light-emitting diode (LED), an avalanche photodiode (APD), a temperature sensor, as well as a bright in-house developed porphyrin molecule. The device was calibrated using a benchtop setup and evaluated in three in vivo animal models. Our findings show that the new device design in combination with the bright porphyrin has the potential to be a useful and accurate tool for measuring $$\hbox {pO}_2$$ pO 2 in tissue, while also highlighting some of the limitations and challenges of oxygen measurements in this context.https://doi.org/10.1038/s41598-023-41917-5 |
| spellingShingle | Lilian Witthauer Emmanuel Roussakis Juan Pedro Cascales Avery Goss Xiaolei Li Alexis Cralley Dor Yoeli Hunter B. Moore Zhaohui Wang Yong Wang Bing Li Christene A. Huang Ernest E. Moore Conor L. Evans Development and in-vivo validation of a portable phosphorescence lifetime-based fiber-optic oxygen sensor Scientific Reports |
| title | Development and in-vivo validation of a portable phosphorescence lifetime-based fiber-optic oxygen sensor |
| title_full | Development and in-vivo validation of a portable phosphorescence lifetime-based fiber-optic oxygen sensor |
| title_fullStr | Development and in-vivo validation of a portable phosphorescence lifetime-based fiber-optic oxygen sensor |
| title_full_unstemmed | Development and in-vivo validation of a portable phosphorescence lifetime-based fiber-optic oxygen sensor |
| title_short | Development and in-vivo validation of a portable phosphorescence lifetime-based fiber-optic oxygen sensor |
| title_sort | development and in vivo validation of a portable phosphorescence lifetime based fiber optic oxygen sensor |
| url | https://doi.org/10.1038/s41598-023-41917-5 |
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