Genome-Wide Characterization of Tomato <i>FAD</i> Gene Family and Expression Analysis under Abiotic Stresses
The fatty acid desaturase (FAD) gene family plays a crucial regulatory role in the resistance process of plant biomembranes. To understand the role of FADs in tomato growth and development, this study identified and analyzed the tomato <i>FAD</i> gene family based on bioinformatics analy...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-11-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/12/22/3818 |
| _version_ | 1797458006835724288 |
|---|---|
| author | Rui Xi Huifang Liu Yijia Chen Hongmei Zhuang Hongwei Han Hao Wang Qiang Wang Ning Li |
| author_facet | Rui Xi Huifang Liu Yijia Chen Hongmei Zhuang Hongwei Han Hao Wang Qiang Wang Ning Li |
| author_sort | Rui Xi |
| collection | DOAJ |
| description | The fatty acid desaturase (FAD) gene family plays a crucial regulatory role in the resistance process of plant biomembranes. To understand the role of FADs in tomato growth and development, this study identified and analyzed the tomato <i>FAD</i> gene family based on bioinformatics analysis methods. In this study, 26 <i>SlFADs</i> were unevenly distributed on 10 chromosomes. Phylogenetic analysis showed that the <i>SlFAD</i> gene family was divided into six branches, and the exon–intron composition and conserved motifs of <i>SlFADs</i> clustered in the same branch were quite conservative. Several hormone and stress response elements in the <i>SlFAD</i> promoter suggest that the expression of <i>SlFAD</i> members is subject to complex regulation; the construction of a tomato FAD protein interaction network found that <i>SlFAD</i> proteins have apparent synergistic effects with SPA and GPAT proteins. qRT-PCR verification results show that <i>SlFAD</i> participates in the expression of tomato root, stem, and leaf tissues; <i>SlFAD8</i> is mainly highly expressed in leaves; <i>SlFAD9</i> plays a vital role in response to salt stress; and <i>SlFAB5</i> regulates all stages of fruit development under the action of exogenous hormones. In summary, this study provides a basis for a systematic understanding of the <i>SlFAD</i> gene family. It provides a theoretical basis for in-depth research on the functional characteristics of tomato <i>SlFAD</i> genes. |
| first_indexed | 2024-03-09T16:31:56Z |
| format | Article |
| id | doaj.art-fcbeecbb67bf4930b9ebbc5dc99bfc06 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T16:31:56Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-fcbeecbb67bf4930b9ebbc5dc99bfc062023-11-24T15:01:53ZengMDPI AGPlants2223-77472023-11-011222381810.3390/plants12223818Genome-Wide Characterization of Tomato <i>FAD</i> Gene Family and Expression Analysis under Abiotic StressesRui Xi0Huifang Liu1Yijia Chen2Hongmei Zhuang3Hongwei Han4Hao Wang5Qiang Wang6Ning Li7Institute of Horticulture Crops, Xinjiang Academy of Agricultural Sciences/Xingjiang Engineering Research Center for Vegetables, Urumqi 830091, ChinaInstitute of Horticulture Crops, Xinjiang Academy of Agricultural Sciences/Xingjiang Engineering Research Center for Vegetables, Urumqi 830091, ChinaInstitute of Horticulture Crops, Xinjiang Academy of Agricultural Sciences/Xingjiang Engineering Research Center for Vegetables, Urumqi 830091, ChinaInstitute of Horticulture Crops, Xinjiang Academy of Agricultural Sciences/Xingjiang Engineering Research Center for Vegetables, Urumqi 830091, ChinaInstitute of Horticulture Crops, Xinjiang Academy of Agricultural Sciences/Xingjiang Engineering Research Center for Vegetables, Urumqi 830091, ChinaInstitute of Horticulture Crops, Xinjiang Academy of Agricultural Sciences/Xingjiang Engineering Research Center for Vegetables, Urumqi 830091, ChinaInstitute of Horticulture Crops, Xinjiang Academy of Agricultural Sciences/Xingjiang Engineering Research Center for Vegetables, Urumqi 830091, ChinaInstitute of Horticulture Crops, Xinjiang Academy of Agricultural Sciences/Xingjiang Engineering Research Center for Vegetables, Urumqi 830091, ChinaThe fatty acid desaturase (FAD) gene family plays a crucial regulatory role in the resistance process of plant biomembranes. To understand the role of FADs in tomato growth and development, this study identified and analyzed the tomato <i>FAD</i> gene family based on bioinformatics analysis methods. In this study, 26 <i>SlFADs</i> were unevenly distributed on 10 chromosomes. Phylogenetic analysis showed that the <i>SlFAD</i> gene family was divided into six branches, and the exon–intron composition and conserved motifs of <i>SlFADs</i> clustered in the same branch were quite conservative. Several hormone and stress response elements in the <i>SlFAD</i> promoter suggest that the expression of <i>SlFAD</i> members is subject to complex regulation; the construction of a tomato FAD protein interaction network found that <i>SlFAD</i> proteins have apparent synergistic effects with SPA and GPAT proteins. qRT-PCR verification results show that <i>SlFAD</i> participates in the expression of tomato root, stem, and leaf tissues; <i>SlFAD8</i> is mainly highly expressed in leaves; <i>SlFAD9</i> plays a vital role in response to salt stress; and <i>SlFAB5</i> regulates all stages of fruit development under the action of exogenous hormones. In summary, this study provides a basis for a systematic understanding of the <i>SlFAD</i> gene family. It provides a theoretical basis for in-depth research on the functional characteristics of tomato <i>SlFAD</i> genes.https://www.mdpi.com/2223-7747/12/22/3818tomatoFADbioinformatics analysisbiotic/abiotic stressesgene expression |
| spellingShingle | Rui Xi Huifang Liu Yijia Chen Hongmei Zhuang Hongwei Han Hao Wang Qiang Wang Ning Li Genome-Wide Characterization of Tomato <i>FAD</i> Gene Family and Expression Analysis under Abiotic Stresses Plants tomato FAD bioinformatics analysis biotic/abiotic stresses gene expression |
| title | Genome-Wide Characterization of Tomato <i>FAD</i> Gene Family and Expression Analysis under Abiotic Stresses |
| title_full | Genome-Wide Characterization of Tomato <i>FAD</i> Gene Family and Expression Analysis under Abiotic Stresses |
| title_fullStr | Genome-Wide Characterization of Tomato <i>FAD</i> Gene Family and Expression Analysis under Abiotic Stresses |
| title_full_unstemmed | Genome-Wide Characterization of Tomato <i>FAD</i> Gene Family and Expression Analysis under Abiotic Stresses |
| title_short | Genome-Wide Characterization of Tomato <i>FAD</i> Gene Family and Expression Analysis under Abiotic Stresses |
| title_sort | genome wide characterization of tomato i fad i gene family and expression analysis under abiotic stresses |
| topic | tomato FAD bioinformatics analysis biotic/abiotic stresses gene expression |
| url | https://www.mdpi.com/2223-7747/12/22/3818 |
| work_keys_str_mv | AT ruixi genomewidecharacterizationoftomatoifadigenefamilyandexpressionanalysisunderabioticstresses AT huifangliu genomewidecharacterizationoftomatoifadigenefamilyandexpressionanalysisunderabioticstresses AT yijiachen genomewidecharacterizationoftomatoifadigenefamilyandexpressionanalysisunderabioticstresses AT hongmeizhuang genomewidecharacterizationoftomatoifadigenefamilyandexpressionanalysisunderabioticstresses AT hongweihan genomewidecharacterizationoftomatoifadigenefamilyandexpressionanalysisunderabioticstresses AT haowang genomewidecharacterizationoftomatoifadigenefamilyandexpressionanalysisunderabioticstresses AT qiangwang genomewidecharacterizationoftomatoifadigenefamilyandexpressionanalysisunderabioticstresses AT ningli genomewidecharacterizationoftomatoifadigenefamilyandexpressionanalysisunderabioticstresses |