Identification of phlebotomine sand flies (Diptera: Psychodidae) from leishmaniasis endemic areas in southeastern Mexico using DNA barcoding
Abstract Leishmaniasis, a vector‐borne disease transmitted to humans through the bite of phlebotomine sand flies, is of public health significance in southeastern Mexico. Active and continuous monitoring of vectors is an important aspect of disease control for the prediction of potential outbreaks....
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-12-01
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| Series: | Ecology and Evolution |
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| Online Access: | https://doi.org/10.1002/ece3.5811 |
| _version_ | 1819177719419633664 |
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| author | Adebiyi A. Adeniran Nadia A. Fernández‐Santos Jorge J. Rodríguez‐Rojas Nancy Treviño‐Garza Heron Huerta‐Jiménez Pedro C. Mis‐Ávila Wilbert A. Pérez‐Pech Luis M. Hernández‐Triana Mario A. Rodríguez‐Pérez |
| author_facet | Adebiyi A. Adeniran Nadia A. Fernández‐Santos Jorge J. Rodríguez‐Rojas Nancy Treviño‐Garza Heron Huerta‐Jiménez Pedro C. Mis‐Ávila Wilbert A. Pérez‐Pech Luis M. Hernández‐Triana Mario A. Rodríguez‐Pérez |
| author_sort | Adebiyi A. Adeniran |
| collection | DOAJ |
| description | Abstract Leishmaniasis, a vector‐borne disease transmitted to humans through the bite of phlebotomine sand flies, is of public health significance in southeastern Mexico. Active and continuous monitoring of vectors is an important aspect of disease control for the prediction of potential outbreaks. Thus, the correct identification of vectors is paramount in this regard. In this study, we employed DNA barcoding as a tool for identifying phlebotomine sand flies collected in localized cutaneous leishmaniasis endemic areas of Quintana Roo, Mexico. Specimens were collected using CDC light and Shannon traps as part of the Mexican Ministry of Health surveillance program. DNA extraction was carried out using a nondestructive protocol, and morphological identification based on taxonomic keys was conducted on slide‐mounted specimens. Molecular taxonomic resolution using the 658‐bp fragment of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene was 100% congruent with the morphological identification. Seven species were identified: Lutzomyia cruciata (Coquillett 1907), Lutzomyia longipalpis (Lutz & Neiva 1912), Psathyromyia shannoni (Dyar 1929), Dampfomyia deleoni (Fairchild & Hertig 1947), Dampfomyia beltrani/steatopyga (Vargas & Díaz‐Nájera 1951), Bichromomyia olmeca olmeca (Vargas & Díaz‐Nájera, 1959), and Brumptomyia mesai (Sherlock 1962). Mean intraspecific divergence ranged from 0.12% to 1.22%, while interspecific distances ranged from 11.59% to 19.29%. Neighbor‐joining (NJ) analysis using the Kimura 2‐parameter model also showed specimens of the same species to be clustered together. The study provides the first cox1 sequences for three species of sand flies and indicates the utility of DNA barcoding for phlebotomine sand flies species identification in southeastern Mexico. |
| first_indexed | 2024-12-22T21:31:07Z |
| format | Article |
| id | doaj.art-fcc0e71b9fe14d8ca03b89dfb1465544 |
| institution | Directory Open Access Journal |
| issn | 2045-7758 |
| language | English |
| last_indexed | 2024-12-22T21:31:07Z |
| publishDate | 2019-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Ecology and Evolution |
| spelling | doaj.art-fcc0e71b9fe14d8ca03b89dfb14655442022-12-21T18:11:55ZengWileyEcology and Evolution2045-77582019-12-01923135431355410.1002/ece3.5811Identification of phlebotomine sand flies (Diptera: Psychodidae) from leishmaniasis endemic areas in southeastern Mexico using DNA barcodingAdebiyi A. Adeniran0Nadia A. Fernández‐Santos1Jorge J. Rodríguez‐Rojas2Nancy Treviño‐Garza3Heron Huerta‐Jiménez4Pedro C. Mis‐Ávila5Wilbert A. Pérez‐Pech6Luis M. Hernández‐Triana7Mario A. Rodríguez‐Pérez8Laboratorio de Biomedicina Molecular Centro de Biotecnología Genómica Instituto Politécnico Nacional Reynosa MéxicoLaboratorio de Biomedicina Molecular Centro de Biotecnología Genómica Instituto Politécnico Nacional Reynosa MéxicoCentro de Investigación y Desarrollo en Ciencias de la Salud Unidad de Patógenos Emergentes, Re-emergentes y Vectores Universidad Autónoma de Nuevo León Nuevo León MéxicoCentro Nacional de Programas Preventivos y Control de Enfermedades Secretaria de Salud Mexico City MéxicoLaboratorio de Entomología e Insectario Instituto Nacional de Diagnóstico y Referencia Epidemiológicos Secretaria de Salud Mexico City MéxicoServicios de Salud del Estado de Quintana Roo Secretaria de Salud Chetumal MéxicoServicios de Salud del Estado de Quintana Roo Secretaria de Salud Chetumal MéxicoAnimal and Plant Health Agency Addlestone UKLaboratorio de Biomedicina Molecular Centro de Biotecnología Genómica Instituto Politécnico Nacional Reynosa MéxicoAbstract Leishmaniasis, a vector‐borne disease transmitted to humans through the bite of phlebotomine sand flies, is of public health significance in southeastern Mexico. Active and continuous monitoring of vectors is an important aspect of disease control for the prediction of potential outbreaks. Thus, the correct identification of vectors is paramount in this regard. In this study, we employed DNA barcoding as a tool for identifying phlebotomine sand flies collected in localized cutaneous leishmaniasis endemic areas of Quintana Roo, Mexico. Specimens were collected using CDC light and Shannon traps as part of the Mexican Ministry of Health surveillance program. DNA extraction was carried out using a nondestructive protocol, and morphological identification based on taxonomic keys was conducted on slide‐mounted specimens. Molecular taxonomic resolution using the 658‐bp fragment of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene was 100% congruent with the morphological identification. Seven species were identified: Lutzomyia cruciata (Coquillett 1907), Lutzomyia longipalpis (Lutz & Neiva 1912), Psathyromyia shannoni (Dyar 1929), Dampfomyia deleoni (Fairchild & Hertig 1947), Dampfomyia beltrani/steatopyga (Vargas & Díaz‐Nájera 1951), Bichromomyia olmeca olmeca (Vargas & Díaz‐Nájera, 1959), and Brumptomyia mesai (Sherlock 1962). Mean intraspecific divergence ranged from 0.12% to 1.22%, while interspecific distances ranged from 11.59% to 19.29%. Neighbor‐joining (NJ) analysis using the Kimura 2‐parameter model also showed specimens of the same species to be clustered together. The study provides the first cox1 sequences for three species of sand flies and indicates the utility of DNA barcoding for phlebotomine sand flies species identification in southeastern Mexico.https://doi.org/10.1002/ece3.5811CO1 geneDNA barcodingMexicomitochondrialsand flies |
| spellingShingle | Adebiyi A. Adeniran Nadia A. Fernández‐Santos Jorge J. Rodríguez‐Rojas Nancy Treviño‐Garza Heron Huerta‐Jiménez Pedro C. Mis‐Ávila Wilbert A. Pérez‐Pech Luis M. Hernández‐Triana Mario A. Rodríguez‐Pérez Identification of phlebotomine sand flies (Diptera: Psychodidae) from leishmaniasis endemic areas in southeastern Mexico using DNA barcoding Ecology and Evolution CO1 gene DNA barcoding Mexico mitochondrial sand flies |
| title | Identification of phlebotomine sand flies (Diptera: Psychodidae) from leishmaniasis endemic areas in southeastern Mexico using DNA barcoding |
| title_full | Identification of phlebotomine sand flies (Diptera: Psychodidae) from leishmaniasis endemic areas in southeastern Mexico using DNA barcoding |
| title_fullStr | Identification of phlebotomine sand flies (Diptera: Psychodidae) from leishmaniasis endemic areas in southeastern Mexico using DNA barcoding |
| title_full_unstemmed | Identification of phlebotomine sand flies (Diptera: Psychodidae) from leishmaniasis endemic areas in southeastern Mexico using DNA barcoding |
| title_short | Identification of phlebotomine sand flies (Diptera: Psychodidae) from leishmaniasis endemic areas in southeastern Mexico using DNA barcoding |
| title_sort | identification of phlebotomine sand flies diptera psychodidae from leishmaniasis endemic areas in southeastern mexico using dna barcoding |
| topic | CO1 gene DNA barcoding Mexico mitochondrial sand flies |
| url | https://doi.org/10.1002/ece3.5811 |
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