The effect of Wogonoside on the expression of TGF-β1/Smad3 in Mycoplasma pneumoniae pneumonia
Screening active compounds from complex herbal extracts is an important part of the drug mechanism research. The present study describes the establishment and application of a sensitive, convenient and efficient method for screening active ingredients targeting pulmonary fibrosis factor TGF-β1 from...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-03-01
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| Series: | Pharmacological Research - Modern Chinese Medicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667142521000178 |
| _version_ | 1818774571411570688 |
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| author | Huihui Wang Xiaoxi Wang Huixing Xu Xin Wang Guixin Tang Yanli Meng Weiming Wang |
| author_facet | Huihui Wang Xiaoxi Wang Huixing Xu Xin Wang Guixin Tang Yanli Meng Weiming Wang |
| author_sort | Huihui Wang |
| collection | DOAJ |
| description | Screening active compounds from complex herbal extracts is an important part of the drug mechanism research. The present study describes the establishment and application of a sensitive, convenient and efficient method for screening active ingredients targeting pulmonary fibrosis factor TGF-β1 from Radix Scutellariae extracts based on the surface plasmon resonance (SPR) biosensors. Previous studies have proved that Qin bai qing fei pellet (Qin bai) can inhibit the expression of TGF-β1 in Mycoplasma pneumoniae pneumonia (MPP). Radix Scutellariae is the main component of Qin bai. However, the mechanism of Radix Scutellariae inhibiting TGF-β1 expression remains unexplained. The study investigated the active compound in Radix Scutellariae which was bound with TGF-β1 by SPR and UPLC-Q-TOF-MS techonology. As a result, Radix Scutellariae extracts showed a good combination with the TGF-β1 in vitro. Wogonoside was identified as a bioactive ingredient by UPLC-Q-TOF-MS. The affinity constant of Wogonoside to TGF-β1 was determined by SPR affinity analysis (KD=21.7 μm). Pharmacology assays revealed that Wogonoside strongly inhibited the expression of TGF-β1 and Smad3 in A549 cells infected by Mycoplasma pneumoniae(M.pneumoniae). Wogonoside prevented Mycoplasma pneumoniae infection by inhibiting the expression of TGF-β1, which might be one of the mechanisms for the anti-MPP properties of Qin bai. These results provided a foundation to further explore the mechanisms of Qin bai. |
| first_indexed | 2024-12-18T10:43:16Z |
| format | Article |
| id | doaj.art-fcc14572d7b740cbb781d09553b8b887 |
| institution | Directory Open Access Journal |
| issn | 2667-1425 |
| language | English |
| last_indexed | 2024-12-18T10:43:16Z |
| publishDate | 2022-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Pharmacological Research - Modern Chinese Medicine |
| spelling | doaj.art-fcc14572d7b740cbb781d09553b8b8872022-12-21T21:10:36ZengElsevierPharmacological Research - Modern Chinese Medicine2667-14252022-03-012100018The effect of Wogonoside on the expression of TGF-β1/Smad3 in Mycoplasma pneumoniae pneumoniaHuihui Wang0Xiaoxi Wang1Huixing Xu2Xin Wang3Guixin Tang4Yanli Meng5Weiming Wang6Heilongjiang Academy of Chinese Medicine Sciences, Harbin, China; Hubei aerospace hospital, Xiaogan, ChinaHeilongjiang Academy of Chinese Medicine Sciences, Harbin, ChinaHeilongjiang Academy of Chinese Medicine Sciences, Harbin, ChinaHeilongjiang Academy of Chinese Medicine Sciences, Harbin, ChinaHarbin Institute of Technology, Harbin, China; Corresponding authors.Heilongjiang Academy of Chinese Medicine Sciences, Harbin, China; Corresponding authors.Southern Medical University, Guangzhou, China; Corresponding authors.Screening active compounds from complex herbal extracts is an important part of the drug mechanism research. The present study describes the establishment and application of a sensitive, convenient and efficient method for screening active ingredients targeting pulmonary fibrosis factor TGF-β1 from Radix Scutellariae extracts based on the surface plasmon resonance (SPR) biosensors. Previous studies have proved that Qin bai qing fei pellet (Qin bai) can inhibit the expression of TGF-β1 in Mycoplasma pneumoniae pneumonia (MPP). Radix Scutellariae is the main component of Qin bai. However, the mechanism of Radix Scutellariae inhibiting TGF-β1 expression remains unexplained. The study investigated the active compound in Radix Scutellariae which was bound with TGF-β1 by SPR and UPLC-Q-TOF-MS techonology. As a result, Radix Scutellariae extracts showed a good combination with the TGF-β1 in vitro. Wogonoside was identified as a bioactive ingredient by UPLC-Q-TOF-MS. The affinity constant of Wogonoside to TGF-β1 was determined by SPR affinity analysis (KD=21.7 μm). Pharmacology assays revealed that Wogonoside strongly inhibited the expression of TGF-β1 and Smad3 in A549 cells infected by Mycoplasma pneumoniae(M.pneumoniae). Wogonoside prevented Mycoplasma pneumoniae infection by inhibiting the expression of TGF-β1, which might be one of the mechanisms for the anti-MPP properties of Qin bai. These results provided a foundation to further explore the mechanisms of Qin bai.http://www.sciencedirect.com/science/article/pii/S2667142521000178Wogonoside1Mycoplasma pneumoniae pneumonia2TGF-β13Surface plasmon resonance4UPLC-Q-TOF-MS5 |
| spellingShingle | Huihui Wang Xiaoxi Wang Huixing Xu Xin Wang Guixin Tang Yanli Meng Weiming Wang The effect of Wogonoside on the expression of TGF-β1/Smad3 in Mycoplasma pneumoniae pneumonia Pharmacological Research - Modern Chinese Medicine Wogonoside1 Mycoplasma pneumoniae pneumonia2 TGF-β13 Surface plasmon resonance4 UPLC-Q-TOF-MS5 |
| title | The effect of Wogonoside on the expression of TGF-β1/Smad3 in Mycoplasma pneumoniae pneumonia |
| title_full | The effect of Wogonoside on the expression of TGF-β1/Smad3 in Mycoplasma pneumoniae pneumonia |
| title_fullStr | The effect of Wogonoside on the expression of TGF-β1/Smad3 in Mycoplasma pneumoniae pneumonia |
| title_full_unstemmed | The effect of Wogonoside on the expression of TGF-β1/Smad3 in Mycoplasma pneumoniae pneumonia |
| title_short | The effect of Wogonoside on the expression of TGF-β1/Smad3 in Mycoplasma pneumoniae pneumonia |
| title_sort | effect of wogonoside on the expression of tgf β1 smad3 in mycoplasma pneumoniae pneumonia |
| topic | Wogonoside1 Mycoplasma pneumoniae pneumonia2 TGF-β13 Surface plasmon resonance4 UPLC-Q-TOF-MS5 |
| url | http://www.sciencedirect.com/science/article/pii/S2667142521000178 |
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