SNX4 in complex with clathrin and dynein: implications for endosome movement.
BACKGROUND:Sorting nexins (SNXs) constitute a family of proteins classified by their phosphatidylinositol (PI) binding Phox homology (PX) domain. Some members regulate intracellular trafficking. We have here investigated mechanisms underlying SNX4 mediated endosome to Golgi transport. METHODOLOGY/PR...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2009-06-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2691479?pdf=render |
| _version_ | 1818386796136890368 |
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| author | Sigrid S Skånland Sébastien Wälchli Andreas Brech Kirsten Sandvig |
| author_facet | Sigrid S Skånland Sébastien Wälchli Andreas Brech Kirsten Sandvig |
| author_sort | Sigrid S Skånland |
| collection | DOAJ |
| description | BACKGROUND:Sorting nexins (SNXs) constitute a family of proteins classified by their phosphatidylinositol (PI) binding Phox homology (PX) domain. Some members regulate intracellular trafficking. We have here investigated mechanisms underlying SNX4 mediated endosome to Golgi transport. METHODOLOGY/PRINCIPAL FINDINGS:We show that SNX4 forms complexes with clathrin and dynein. The interactions were inhibited by wortmannin, a PI3-kinase inhibitor, suggesting that they form when SNX4 is associated with PI(3)P on endosomes. We further localized the clathrin interacting site on SNX4 to a clathrin box variant. A short peptide containing this motif was sufficient to pull down both clathrin and dynein. Knockdown studies demonstrated that clathrin is not required for the SNX4/dynein interaction. Moreover, clathrin knockdown led to increased Golgi transport of the toxin ricin, as well as redistribution of endosomes. CONCLUSIONS/SIGNIFICANCE:We discuss the possibility of clathrin serving as a regulator of SNX4-dependent transport. Upon clathrin release, dynein may bind SNX4 and mediate retrograde movement. |
| first_indexed | 2024-12-14T03:59:44Z |
| format | Article |
| id | doaj.art-fcc1938a16f94e7ca0e477b0b457d0eb |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-14T03:59:44Z |
| publishDate | 2009-06-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-fcc1938a16f94e7ca0e477b0b457d0eb2022-12-21T23:18:00ZengPublic Library of Science (PLoS)PLoS ONE1932-62032009-06-0146e593510.1371/journal.pone.0005935SNX4 in complex with clathrin and dynein: implications for endosome movement.Sigrid S SkånlandSébastien WälchliAndreas BrechKirsten SandvigBACKGROUND:Sorting nexins (SNXs) constitute a family of proteins classified by their phosphatidylinositol (PI) binding Phox homology (PX) domain. Some members regulate intracellular trafficking. We have here investigated mechanisms underlying SNX4 mediated endosome to Golgi transport. METHODOLOGY/PRINCIPAL FINDINGS:We show that SNX4 forms complexes with clathrin and dynein. The interactions were inhibited by wortmannin, a PI3-kinase inhibitor, suggesting that they form when SNX4 is associated with PI(3)P on endosomes. We further localized the clathrin interacting site on SNX4 to a clathrin box variant. A short peptide containing this motif was sufficient to pull down both clathrin and dynein. Knockdown studies demonstrated that clathrin is not required for the SNX4/dynein interaction. Moreover, clathrin knockdown led to increased Golgi transport of the toxin ricin, as well as redistribution of endosomes. CONCLUSIONS/SIGNIFICANCE:We discuss the possibility of clathrin serving as a regulator of SNX4-dependent transport. Upon clathrin release, dynein may bind SNX4 and mediate retrograde movement.http://europepmc.org/articles/PMC2691479?pdf=render |
| spellingShingle | Sigrid S Skånland Sébastien Wälchli Andreas Brech Kirsten Sandvig SNX4 in complex with clathrin and dynein: implications for endosome movement. PLoS ONE |
| title | SNX4 in complex with clathrin and dynein: implications for endosome movement. |
| title_full | SNX4 in complex with clathrin and dynein: implications for endosome movement. |
| title_fullStr | SNX4 in complex with clathrin and dynein: implications for endosome movement. |
| title_full_unstemmed | SNX4 in complex with clathrin and dynein: implications for endosome movement. |
| title_short | SNX4 in complex with clathrin and dynein: implications for endosome movement. |
| title_sort | snx4 in complex with clathrin and dynein implications for endosome movement |
| url | http://europepmc.org/articles/PMC2691479?pdf=render |
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