High Expression of a tRNA<sup>Pro</sup> Derivative Associates with Poor Survival and Independently Predicts Colorectal Cancer Recurrence
Colorectal cancer (CRC) is the second most lethal cause of cancer-related deaths in Europe. Fragments of tRNA<sup>Pro</sup> are conserved among vertebrates, characterized by pleiotropic regulatory functions and have been found to discriminate colorectal tumors from normal colorectal muco...
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MDPI AG
2022-05-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/10/5/1120 |
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| author | Panagiotis Tsiakanikas Panagiotis G. Adamopoulos Dimitra Tsirba Pinelopi I. Artemaki Iordanis N. Papadopoulos Christos K. Kontos Andreas Scorilas |
| author_facet | Panagiotis Tsiakanikas Panagiotis G. Adamopoulos Dimitra Tsirba Pinelopi I. Artemaki Iordanis N. Papadopoulos Christos K. Kontos Andreas Scorilas |
| author_sort | Panagiotis Tsiakanikas |
| collection | DOAJ |
| description | Colorectal cancer (CRC) is the second most lethal cause of cancer-related deaths in Europe. Fragments of tRNA<sup>Pro</sup> are conserved among vertebrates, characterized by pleiotropic regulatory functions and have been found to discriminate colorectal tumors from normal colorectal mucosa. In the current study, we investigated the prognostic utility of 5′-tiRNA-Pro<sup>TGG</sup> levels in CRC. For this purpose, total RNA was extracted from 155 malignant colorectal tumors and 74 adjacent non-cancerous tissue specimens, polyadenylated and reverse-transcribed using an oligo-dT adapter as primer. Real-time quantitative PCR (qPCR) was used to assess the levels of 5′-tiRNA-Pro<sup>TGG</sup>. Kaplan-Meier survival analysis demonstrated that high 5′-tiRNA-Pro<sup>TGG</sup> levels predict both poor disease-free survival (DFS) and overall survival (OS) of CRC patients. Of note, high 5′-tiRNA-Pro<sup>TGG</sup> levels retain their unfavorable prognostic value in patients with rectal cancer and/or moderately differentiated CRC (grade II). More importantly, multivariate cox regression analysis highlighted that the overexpression of 5′-tiRNA-Pro<sup>TGG</sup> constitutes an adverse prognostic factor predicting short-term relapse of CRC patients independently of the established prognosticators in CRC. Finally, bioinformatics analysis unveiled a potentially critical role of 5′-tiRNA-Pro<sup>TGG</sup> regarding the maintenance of cellular homeostasis, signaling, cell communication, and cellular motility. |
| first_indexed | 2024-03-10T03:18:02Z |
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| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-10T03:18:02Z |
| publishDate | 2022-05-01 |
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| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-fcc4d410ad5a4491a6877065e05051a62023-11-23T10:11:20ZengMDPI AGBiomedicines2227-90592022-05-01105112010.3390/biomedicines10051120High Expression of a tRNA<sup>Pro</sup> Derivative Associates with Poor Survival and Independently Predicts Colorectal Cancer RecurrencePanagiotis Tsiakanikas0Panagiotis G. Adamopoulos1Dimitra Tsirba2Pinelopi I. Artemaki3Iordanis N. Papadopoulos4Christos K. Kontos5Andreas Scorilas6Department of Biochemistry and Molecular Biology, Faculty of Biology, National and Kapodistrian University of Athens, 15701 Athens, GreeceDepartment of Biochemistry and Molecular Biology, Faculty of Biology, National and Kapodistrian University of Athens, 15701 Athens, GreeceDepartment of Biochemistry and Molecular Biology, Faculty of Biology, National and Kapodistrian University of Athens, 15701 Athens, GreeceDepartment of Biochemistry and Molecular Biology, Faculty of Biology, National and Kapodistrian University of Athens, 15701 Athens, GreeceFourth Surgery Department, National and Kapodistrian University of Athens, University General Hospital “Attikon”, 12462 Athens, GreeceDepartment of Biochemistry and Molecular Biology, Faculty of Biology, National and Kapodistrian University of Athens, 15701 Athens, GreeceDepartment of Biochemistry and Molecular Biology, Faculty of Biology, National and Kapodistrian University of Athens, 15701 Athens, GreeceColorectal cancer (CRC) is the second most lethal cause of cancer-related deaths in Europe. Fragments of tRNA<sup>Pro</sup> are conserved among vertebrates, characterized by pleiotropic regulatory functions and have been found to discriminate colorectal tumors from normal colorectal mucosa. In the current study, we investigated the prognostic utility of 5′-tiRNA-Pro<sup>TGG</sup> levels in CRC. For this purpose, total RNA was extracted from 155 malignant colorectal tumors and 74 adjacent non-cancerous tissue specimens, polyadenylated and reverse-transcribed using an oligo-dT adapter as primer. Real-time quantitative PCR (qPCR) was used to assess the levels of 5′-tiRNA-Pro<sup>TGG</sup>. Kaplan-Meier survival analysis demonstrated that high 5′-tiRNA-Pro<sup>TGG</sup> levels predict both poor disease-free survival (DFS) and overall survival (OS) of CRC patients. Of note, high 5′-tiRNA-Pro<sup>TGG</sup> levels retain their unfavorable prognostic value in patients with rectal cancer and/or moderately differentiated CRC (grade II). More importantly, multivariate cox regression analysis highlighted that the overexpression of 5′-tiRNA-Pro<sup>TGG</sup> constitutes an adverse prognostic factor predicting short-term relapse of CRC patients independently of the established prognosticators in CRC. Finally, bioinformatics analysis unveiled a potentially critical role of 5′-tiRNA-Pro<sup>TGG</sup> regarding the maintenance of cellular homeostasis, signaling, cell communication, and cellular motility.https://www.mdpi.com/2227-9059/10/5/1120tRNA-derived small RNA (tsRNA)tRNA-derived fragment (tRF)tRNA halfmolecular tumor biomarkersprognosiscolon carcinoma |
| spellingShingle | Panagiotis Tsiakanikas Panagiotis G. Adamopoulos Dimitra Tsirba Pinelopi I. Artemaki Iordanis N. Papadopoulos Christos K. Kontos Andreas Scorilas High Expression of a tRNA<sup>Pro</sup> Derivative Associates with Poor Survival and Independently Predicts Colorectal Cancer Recurrence Biomedicines tRNA-derived small RNA (tsRNA) tRNA-derived fragment (tRF) tRNA half molecular tumor biomarkers prognosis colon carcinoma |
| title | High Expression of a tRNA<sup>Pro</sup> Derivative Associates with Poor Survival and Independently Predicts Colorectal Cancer Recurrence |
| title_full | High Expression of a tRNA<sup>Pro</sup> Derivative Associates with Poor Survival and Independently Predicts Colorectal Cancer Recurrence |
| title_fullStr | High Expression of a tRNA<sup>Pro</sup> Derivative Associates with Poor Survival and Independently Predicts Colorectal Cancer Recurrence |
| title_full_unstemmed | High Expression of a tRNA<sup>Pro</sup> Derivative Associates with Poor Survival and Independently Predicts Colorectal Cancer Recurrence |
| title_short | High Expression of a tRNA<sup>Pro</sup> Derivative Associates with Poor Survival and Independently Predicts Colorectal Cancer Recurrence |
| title_sort | high expression of a trna sup pro sup derivative associates with poor survival and independently predicts colorectal cancer recurrence |
| topic | tRNA-derived small RNA (tsRNA) tRNA-derived fragment (tRF) tRNA half molecular tumor biomarkers prognosis colon carcinoma |
| url | https://www.mdpi.com/2227-9059/10/5/1120 |
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