Nonlinear master relation in microscopic mechanical response of semiflexible biopolymer networks
A network of semiflexible biopolymers, known as the cytoskeleton, and molecular motors play fundamental mechanical roles in cellular activities. The cytoskeletal response to forces generated by molecular motors is profoundly linked to physiological processes. However, owing to the highly nonlinear m...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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IOP Publishing
2022-01-01
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| Series: | New Journal of Physics |
| Subjects: | |
| Online Access: | https://doi.org/10.1088/1367-2630/ac6902 |
| _version_ | 1797748464315006976 |
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| author | N Honda K Shiraki F van Esterik S Inokuchi H Ebata D Mizuno |
| author_facet | N Honda K Shiraki F van Esterik S Inokuchi H Ebata D Mizuno |
| author_sort | N Honda |
| collection | DOAJ |
| description | A network of semiflexible biopolymers, known as the cytoskeleton, and molecular motors play fundamental mechanical roles in cellular activities. The cytoskeletal response to forces generated by molecular motors is profoundly linked to physiological processes. However, owing to the highly nonlinear mechanical properties, the cytoskeletal response on the microscopic level is largely elusive. The aim of this study is to investigate the microscopic mechanical response of semiflexible biopolymer networks by conducting microrheology (MR) experiments. Micrometer-sized colloidal particles, embedded in semiflexible biopolymer networks, were forced beyond the linear regime at a variety of conditions by using feedback-controlled optical trapping. This high-bandwidth MR technology revealed an affine elastic response, which showed stiffening upon local forcing. After scaling the stiffening behaviors, with parameters describing semiflexible networks, a collapse onto a single master curve was observed. The physics underlying the general microscopic response is presented to justify the collapse, and its potentials/implications to elucidate cell mechanics is discussed. |
| first_indexed | 2024-03-12T16:06:08Z |
| format | Article |
| id | doaj.art-fcca9da161774f579a569a37bf8aa53a |
| institution | Directory Open Access Journal |
| issn | 1367-2630 |
| language | English |
| last_indexed | 2024-03-12T16:06:08Z |
| publishDate | 2022-01-01 |
| publisher | IOP Publishing |
| record_format | Article |
| series | New Journal of Physics |
| spelling | doaj.art-fcca9da161774f579a569a37bf8aa53a2023-08-09T14:23:10ZengIOP PublishingNew Journal of Physics1367-26302022-01-0124505303110.1088/1367-2630/ac6902Nonlinear master relation in microscopic mechanical response of semiflexible biopolymer networksN Honda0K Shiraki1F van Esterik2S Inokuchi3H Ebata4D Mizuno5https://orcid.org/0000-0001-7749-3681Department of Physics, Kyushu University , 819-0395 Fukuoka, JapanDepartment of Physics, Kyushu University , 819-0395 Fukuoka, JapanDepartment of Physics, Kyushu University , 819-0395 Fukuoka, JapanDepartment of Physics, Kyushu University , 819-0395 Fukuoka, JapanDepartment of Physics, Kyushu University , 819-0395 Fukuoka, JapanDepartment of Physics, Kyushu University , 819-0395 Fukuoka, JapanA network of semiflexible biopolymers, known as the cytoskeleton, and molecular motors play fundamental mechanical roles in cellular activities. The cytoskeletal response to forces generated by molecular motors is profoundly linked to physiological processes. However, owing to the highly nonlinear mechanical properties, the cytoskeletal response on the microscopic level is largely elusive. The aim of this study is to investigate the microscopic mechanical response of semiflexible biopolymer networks by conducting microrheology (MR) experiments. Micrometer-sized colloidal particles, embedded in semiflexible biopolymer networks, were forced beyond the linear regime at a variety of conditions by using feedback-controlled optical trapping. This high-bandwidth MR technology revealed an affine elastic response, which showed stiffening upon local forcing. After scaling the stiffening behaviors, with parameters describing semiflexible networks, a collapse onto a single master curve was observed. The physics underlying the general microscopic response is presented to justify the collapse, and its potentials/implications to elucidate cell mechanics is discussed.https://doi.org/10.1088/1367-2630/ac6902microrheologybiopolymer networkstress stiffening |
| spellingShingle | N Honda K Shiraki F van Esterik S Inokuchi H Ebata D Mizuno Nonlinear master relation in microscopic mechanical response of semiflexible biopolymer networks New Journal of Physics microrheology biopolymer network stress stiffening |
| title | Nonlinear master relation in microscopic mechanical response of semiflexible biopolymer networks |
| title_full | Nonlinear master relation in microscopic mechanical response of semiflexible biopolymer networks |
| title_fullStr | Nonlinear master relation in microscopic mechanical response of semiflexible biopolymer networks |
| title_full_unstemmed | Nonlinear master relation in microscopic mechanical response of semiflexible biopolymer networks |
| title_short | Nonlinear master relation in microscopic mechanical response of semiflexible biopolymer networks |
| title_sort | nonlinear master relation in microscopic mechanical response of semiflexible biopolymer networks |
| topic | microrheology biopolymer network stress stiffening |
| url | https://doi.org/10.1088/1367-2630/ac6902 |
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