NK, NKT and Invariant-NKT Cells in Tumor Draining Lymph Nodes of Patients with Breast Cancer
<strong>Background:</strong> NK (natural killer) and NKT (natural killer T) cells, as components of innate immune system, play a crucial role in tumor progression and dissemination. <strong>Objective:</strong> To investigate the percentages of NK cells, NKT cells, iNKT (invar...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Shiraz University of Medical Sciences
2019-12-01
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| Series: | Iranian Journal of Immunology |
| Subjects: | |
| Online Access: | http://iji.sums.ac.ir/article_45971_ed76f9f0e72079ec2206ccf1415204b0.pdf |
| _version_ | 1828451114637328384 |
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| author | Somayeh Rezaeifard Akbar Safaei Abdolrasoul Talei Zahra Faghih Nasrollah Erfani |
| author_facet | Somayeh Rezaeifard Akbar Safaei Abdolrasoul Talei Zahra Faghih Nasrollah Erfani |
| author_sort | Somayeh Rezaeifard |
| collection | DOAJ |
| description | <strong>Background:</strong> NK (natural killer) and NKT (natural killer T) cells, as components of innate immune system, play a crucial role in tumor progression and dissemination. <strong>Objective:</strong> To investigate the percentages of NK cells, NKT cells, iNKT (invariant natural killer T) cells, total T lymphocytes as well as activated T lymphocytes, in tumor draining lymph nodes (TDLNs) of patients with breast cancer (BC) and their association with different clinic-pathological features of the patients. <strong>Methods:</strong> Axillary lymph nodes were obtained from 30 Iranian women with breast cancer. After routine pathological evaluations, mononuclear cells were separated from their lymph nodes and incubated with appropriate fluorochrome conjugated monoclonal antibodies specific for CD3, HLA-DR, CD16/56, and Vα24Jα18-TCR. Data were collected on a four-color flow cytometer and analyzed by CellQuest software. <strong>Results:</strong> The mean percentages of NK (CD3<sup>-</sup>CD16/56<sup>+</sup>), NKT (CD3<sup>+</sup>CD16/56<sup>+</sup>) and iNKT (Vα24Jα18-TCR<sup>+</sup>) cells in TDLNs mononuclear cells of BC patients were 2.04%, 2.44% and 0.1%, respectively. A significant decrease in the percentages of NK and iNKT subsets in patients with grade I was observed compared to grade III (p=0.03 and p=0.01, respectively). Moreover, NK cells were increased in patients with grade III of BC compared to grade II (p= 0.003). <strong>Conclusion:</strong> The increase in the percentage of NK and iNKT cells in TDLNs of patients with higher grade of BC might suggest a suppressive phenotype for these cells in breast cancer, which merit more functional investigation. |
| first_indexed | 2024-12-10T23:32:16Z |
| format | Article |
| id | doaj.art-fccd515b655444cd947b70fc824aba22 |
| institution | Directory Open Access Journal |
| issn | 1735-1383 1735-367X |
| language | English |
| last_indexed | 2024-12-10T23:32:16Z |
| publishDate | 2019-12-01 |
| publisher | Shiraz University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Immunology |
| spelling | doaj.art-fccd515b655444cd947b70fc824aba222022-12-22T01:29:21ZengShiraz University of Medical SciencesIranian Journal of Immunology1735-13831735-367X2019-12-0116429129810.22034/iji.2019.8028045971NK, NKT and Invariant-NKT Cells in Tumor Draining Lymph Nodes of Patients with Breast CancerSomayeh Rezaeifard0Akbar Safaei1Abdolrasoul Talei2Zahra Faghih3Nasrollah Erfani4Cancer Immunology Group, Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranBreast Diseases Research Center, Shiraz University of Medical Sciences, Shiraz, IranCancer Immunology Group, Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, IranCancer Immunology Group, Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran |Department of Immunology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran<strong>Background:</strong> NK (natural killer) and NKT (natural killer T) cells, as components of innate immune system, play a crucial role in tumor progression and dissemination. <strong>Objective:</strong> To investigate the percentages of NK cells, NKT cells, iNKT (invariant natural killer T) cells, total T lymphocytes as well as activated T lymphocytes, in tumor draining lymph nodes (TDLNs) of patients with breast cancer (BC) and their association with different clinic-pathological features of the patients. <strong>Methods:</strong> Axillary lymph nodes were obtained from 30 Iranian women with breast cancer. After routine pathological evaluations, mononuclear cells were separated from their lymph nodes and incubated with appropriate fluorochrome conjugated monoclonal antibodies specific for CD3, HLA-DR, CD16/56, and Vα24Jα18-TCR. Data were collected on a four-color flow cytometer and analyzed by CellQuest software. <strong>Results:</strong> The mean percentages of NK (CD3<sup>-</sup>CD16/56<sup>+</sup>), NKT (CD3<sup>+</sup>CD16/56<sup>+</sup>) and iNKT (Vα24Jα18-TCR<sup>+</sup>) cells in TDLNs mononuclear cells of BC patients were 2.04%, 2.44% and 0.1%, respectively. A significant decrease in the percentages of NK and iNKT subsets in patients with grade I was observed compared to grade III (p=0.03 and p=0.01, respectively). Moreover, NK cells were increased in patients with grade III of BC compared to grade II (p= 0.003). <strong>Conclusion:</strong> The increase in the percentage of NK and iNKT cells in TDLNs of patients with higher grade of BC might suggest a suppressive phenotype for these cells in breast cancer, which merit more functional investigation.http://iji.sums.ac.ir/article_45971_ed76f9f0e72079ec2206ccf1415204b0.pdfbreast cancerlymph nodesinvariant-nkt cellsnk cellsnkt cells |
| spellingShingle | Somayeh Rezaeifard Akbar Safaei Abdolrasoul Talei Zahra Faghih Nasrollah Erfani NK, NKT and Invariant-NKT Cells in Tumor Draining Lymph Nodes of Patients with Breast Cancer Iranian Journal of Immunology breast cancer lymph nodes invariant-nkt cells nk cells nkt cells |
| title | NK, NKT and Invariant-NKT Cells in Tumor Draining Lymph Nodes of Patients with Breast Cancer |
| title_full | NK, NKT and Invariant-NKT Cells in Tumor Draining Lymph Nodes of Patients with Breast Cancer |
| title_fullStr | NK, NKT and Invariant-NKT Cells in Tumor Draining Lymph Nodes of Patients with Breast Cancer |
| title_full_unstemmed | NK, NKT and Invariant-NKT Cells in Tumor Draining Lymph Nodes of Patients with Breast Cancer |
| title_short | NK, NKT and Invariant-NKT Cells in Tumor Draining Lymph Nodes of Patients with Breast Cancer |
| title_sort | nk nkt and invariant nkt cells in tumor draining lymph nodes of patients with breast cancer |
| topic | breast cancer lymph nodes invariant-nkt cells nk cells nkt cells |
| url | http://iji.sums.ac.ir/article_45971_ed76f9f0e72079ec2206ccf1415204b0.pdf |
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