Symbiosis of Arbuscular Mycorrhizal Fungi and <i>Lycium barbarum</i> L. Prefers NO<sub>3</sub><sup>−</sup> over NH<sub>4</sub><sup>+</sup>
Nitrogen (N) is an essential nutrient that plants require and is, most of the time, limited in different terrestrial ecosystems. Forming symbioses with plants, arbuscular mycorrhizal (AM) fungi improve mineral element uptake and the net primary production of plants. Recent reports have suggested tha...
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MDPI AG
2023-05-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/9/6/637 |
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| author | Minggui Gong Qiaoming Zhang Kang Cheng Haoqiang Zhang |
| author_facet | Minggui Gong Qiaoming Zhang Kang Cheng Haoqiang Zhang |
| author_sort | Minggui Gong |
| collection | DOAJ |
| description | Nitrogen (N) is an essential nutrient that plants require and is, most of the time, limited in different terrestrial ecosystems. Forming symbioses with plants, arbuscular mycorrhizal (AM) fungi improve mineral element uptake and the net primary production of plants. Recent reports have suggested that AM fungi mediate N uptake in plants. However, there are fewer studies on the influence of AM fungi on the response of <i>Lycium barbarum</i>, a medicinal plant in northwest China, under different N-addition conditions. In this study, the effect of <i>Rhizophagus irregularis</i>, N forms (NO<sub>3</sub><sup>−</sup> and NH<sub>4</sub><sup>+</sup>), and N levels (1.5, 7.5, 15, 30 mM) on the performance of <i>L. barbarum</i> was evaluated through a pot experiment. The application of <i>R. irregularis</i> significantly improved <i>L. barbarum</i> biomass, net photosynthetic rate, and root tissue viability under adequate NO<sub>3</sub><sup>−</sup> and NH<sub>4</sub><sup>+</sup> supplies, and mycorrhizal plants showed better performance under NO<sub>3</sub><sup>−</sup> supply. AM colonization enhanced N acquisition under adequate NO<sub>3</sub><sup>−</sup> supply and strongly induced the expression of <i>LbAMT3-1</i> in <i>L. barbarum</i> roots. Based on these results, we propose that NO<sub>3</sub><sup>−</sup>-dominated N supply favors mycorrhizal symbiosis to a greater extent than NH<sub>4</sub><sup>+</sup>; this study provides a basis for maintaining beneficial AM symbiosis during nitrogen fertilizer use in arable land. |
| first_indexed | 2024-03-11T02:24:01Z |
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| language | English |
| last_indexed | 2024-03-11T02:24:01Z |
| publishDate | 2023-05-01 |
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| series | Horticulturae |
| spelling | doaj.art-fcd36b290c1748ae814cc1c0ef42d0802023-11-18T10:40:32ZengMDPI AGHorticulturae2311-75242023-05-019663710.3390/horticulturae9060637Symbiosis of Arbuscular Mycorrhizal Fungi and <i>Lycium barbarum</i> L. Prefers NO<sub>3</sub><sup>−</sup> over NH<sub>4</sub><sup>+</sup>Minggui Gong0Qiaoming Zhang1Kang Cheng2Haoqiang Zhang3College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471023, ChinaCollege of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471023, ChinaCollege of Forestry, Northwest A & F University, Yangling, Xianyang 712100, ChinaCollege of Forestry, Northwest A & F University, Yangling, Xianyang 712100, ChinaNitrogen (N) is an essential nutrient that plants require and is, most of the time, limited in different terrestrial ecosystems. Forming symbioses with plants, arbuscular mycorrhizal (AM) fungi improve mineral element uptake and the net primary production of plants. Recent reports have suggested that AM fungi mediate N uptake in plants. However, there are fewer studies on the influence of AM fungi on the response of <i>Lycium barbarum</i>, a medicinal plant in northwest China, under different N-addition conditions. In this study, the effect of <i>Rhizophagus irregularis</i>, N forms (NO<sub>3</sub><sup>−</sup> and NH<sub>4</sub><sup>+</sup>), and N levels (1.5, 7.5, 15, 30 mM) on the performance of <i>L. barbarum</i> was evaluated through a pot experiment. The application of <i>R. irregularis</i> significantly improved <i>L. barbarum</i> biomass, net photosynthetic rate, and root tissue viability under adequate NO<sub>3</sub><sup>−</sup> and NH<sub>4</sub><sup>+</sup> supplies, and mycorrhizal plants showed better performance under NO<sub>3</sub><sup>−</sup> supply. AM colonization enhanced N acquisition under adequate NO<sub>3</sub><sup>−</sup> supply and strongly induced the expression of <i>LbAMT3-1</i> in <i>L. barbarum</i> roots. Based on these results, we propose that NO<sub>3</sub><sup>−</sup>-dominated N supply favors mycorrhizal symbiosis to a greater extent than NH<sub>4</sub><sup>+</sup>; this study provides a basis for maintaining beneficial AM symbiosis during nitrogen fertilizer use in arable land.https://www.mdpi.com/2311-7524/9/6/637arbuscular mycorrhizal<i>Lycium barbarum</i>ammoniumnitrateammonium transporter |
| spellingShingle | Minggui Gong Qiaoming Zhang Kang Cheng Haoqiang Zhang Symbiosis of Arbuscular Mycorrhizal Fungi and <i>Lycium barbarum</i> L. Prefers NO<sub>3</sub><sup>−</sup> over NH<sub>4</sub><sup>+</sup> Horticulturae arbuscular mycorrhizal <i>Lycium barbarum</i> ammonium nitrate ammonium transporter |
| title | Symbiosis of Arbuscular Mycorrhizal Fungi and <i>Lycium barbarum</i> L. Prefers NO<sub>3</sub><sup>−</sup> over NH<sub>4</sub><sup>+</sup> |
| title_full | Symbiosis of Arbuscular Mycorrhizal Fungi and <i>Lycium barbarum</i> L. Prefers NO<sub>3</sub><sup>−</sup> over NH<sub>4</sub><sup>+</sup> |
| title_fullStr | Symbiosis of Arbuscular Mycorrhizal Fungi and <i>Lycium barbarum</i> L. Prefers NO<sub>3</sub><sup>−</sup> over NH<sub>4</sub><sup>+</sup> |
| title_full_unstemmed | Symbiosis of Arbuscular Mycorrhizal Fungi and <i>Lycium barbarum</i> L. Prefers NO<sub>3</sub><sup>−</sup> over NH<sub>4</sub><sup>+</sup> |
| title_short | Symbiosis of Arbuscular Mycorrhizal Fungi and <i>Lycium barbarum</i> L. Prefers NO<sub>3</sub><sup>−</sup> over NH<sub>4</sub><sup>+</sup> |
| title_sort | symbiosis of arbuscular mycorrhizal fungi and i lycium barbarum i l prefers no sub 3 sub sup sup over nh sub 4 sub sup sup |
| topic | arbuscular mycorrhizal <i>Lycium barbarum</i> ammonium nitrate ammonium transporter |
| url | https://www.mdpi.com/2311-7524/9/6/637 |
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