<i>Rhizoctonia solani</i> AG-3PT is the major pathogen associated with potato stem canker and black scurf in Jordan
<italic>Rhizoctonia solani</italic> (teleomorph: <italic>Thanatephorus cucumeris</italic>) is a global soil-borne pathogen that severely harms potato crops, leading to significant product losses. Black scurf and stem canker are two manifestations caused by this pathogen, with...
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| Format: | Article |
| Language: | English |
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AIMS Press
2023-01-01
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| Series: | AIMS Agriculture and Food |
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| Online Access: | https://www.aimspress.com/article/doi/10.3934/agrfood.2023006?viewType=HTML |
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| author | Ziad Jaradat Hussen Aldakil Maher Tadros Mohammad Alboom Batool Khataybeh |
| author_facet | Ziad Jaradat Hussen Aldakil Maher Tadros Mohammad Alboom Batool Khataybeh |
| author_sort | Ziad Jaradat |
| collection | DOAJ |
| description | <italic>Rhizoctonia solani</italic> (teleomorph: <italic>Thanatephorus cucumeris</italic>) is a global soil-borne pathogen that severely harms potato crops, leading to significant product losses. Black scurf and stem canker are two manifestations caused by this pathogen, with variable intensity based on the distinctive anastomosis group endemic to the region. During the growing season of 2017 (March and April), 57 different fungus isolates were collected from potato crops farmed in the Jordan Valley. The identity of all the isolates was confirmed by sequencing the internal transcribed spacer (ITS) of the ribosomal DNA gene, and the hyphal interactions were also performed with <italic>R. solani</italic> isolates. The sequences were deposited in GenBank, where accession numbers were obtained. 21 of the isolates were AG-3PT <italic>R. solani</italic>, with 98–99% identity to reference strains. Somatic compatibility was determined by hyphal interactions, which showed pairing compatibility among the isolates. Around 86.7% of the pairings were somatically incompatible, indicating a high level of genetic diversity among the isolates, while only 13.3% of the pairings were somatically compatible. Testing for pathogenicity revealed that AG-3PT affected the stems of solanaceous plants, including potatoes, and the roots of other plant species. Based on the findings of this study, <italic>R. solani</italic> AG-3PT was the primary pathogen associated with potato stem canker and black scurf diseases in Jordan. To our knowledge, this is the first report on this pathogen's isolation and identification in Jordan. |
| first_indexed | 2024-04-09T21:35:20Z |
| format | Article |
| id | doaj.art-fcdaa3a5223a422f9498e7d48aeda002 |
| institution | Directory Open Access Journal |
| issn | 2471-2086 |
| language | English |
| last_indexed | 2024-04-09T21:35:20Z |
| publishDate | 2023-01-01 |
| publisher | AIMS Press |
| record_format | Article |
| series | AIMS Agriculture and Food |
| spelling | doaj.art-fcdaa3a5223a422f9498e7d48aeda0022023-03-27T01:26:42ZengAIMS PressAIMS Agriculture and Food2471-20862023-01-018111913610.3934/agrfood.2023006<i>Rhizoctonia solani</i> AG-3PT is the major pathogen associated with potato stem canker and black scurf in JordanZiad Jaradat 0Hussen Aldakil1Maher Tadros2Mohammad Alboom3Batool Khataybeh 41. Department of Biotechnology and Genetic Engineering, Jordan University of Science and Technology, P. O Box, 3030, Irbid, Jordan2. Department of Plant Production, Jordan University of Science and Technology, P. O Box, 3030, Irbid, Jordan3. Department of Natural Resources and Environment, Jordan University of Science and Technology, P. O Box, 3030, Irbid, Jordan1. Department of Biotechnology and Genetic Engineering, Jordan University of Science and Technology, P. O Box, 3030, Irbid, Jordan4. Department of Nutrition and Food Technology, Jordan University of Science and Technology, P. O Box, 3030, Irbid, Jordan<italic>Rhizoctonia solani</italic> (teleomorph: <italic>Thanatephorus cucumeris</italic>) is a global soil-borne pathogen that severely harms potato crops, leading to significant product losses. Black scurf and stem canker are two manifestations caused by this pathogen, with variable intensity based on the distinctive anastomosis group endemic to the region. During the growing season of 2017 (March and April), 57 different fungus isolates were collected from potato crops farmed in the Jordan Valley. The identity of all the isolates was confirmed by sequencing the internal transcribed spacer (ITS) of the ribosomal DNA gene, and the hyphal interactions were also performed with <italic>R. solani</italic> isolates. The sequences were deposited in GenBank, where accession numbers were obtained. 21 of the isolates were AG-3PT <italic>R. solani</italic>, with 98–99% identity to reference strains. Somatic compatibility was determined by hyphal interactions, which showed pairing compatibility among the isolates. Around 86.7% of the pairings were somatically incompatible, indicating a high level of genetic diversity among the isolates, while only 13.3% of the pairings were somatically compatible. Testing for pathogenicity revealed that AG-3PT affected the stems of solanaceous plants, including potatoes, and the roots of other plant species. Based on the findings of this study, <italic>R. solani</italic> AG-3PT was the primary pathogen associated with potato stem canker and black scurf diseases in Jordan. To our knowledge, this is the first report on this pathogen's isolation and identification in Jordan.https://www.aimspress.com/article/doi/10.3934/agrfood.2023006?viewType=HTMLblack scurfpathogenicityrhizoctonia solanistem cankerthanatephorus cucumeris |
| spellingShingle | Ziad Jaradat Hussen Aldakil Maher Tadros Mohammad Alboom Batool Khataybeh <i>Rhizoctonia solani</i> AG-3PT is the major pathogen associated with potato stem canker and black scurf in Jordan AIMS Agriculture and Food black scurf pathogenicity rhizoctonia solani stem canker thanatephorus cucumeris |
| title | <i>Rhizoctonia solani</i> AG-3PT is the major pathogen associated with potato stem canker and black scurf in Jordan |
| title_full | <i>Rhizoctonia solani</i> AG-3PT is the major pathogen associated with potato stem canker and black scurf in Jordan |
| title_fullStr | <i>Rhizoctonia solani</i> AG-3PT is the major pathogen associated with potato stem canker and black scurf in Jordan |
| title_full_unstemmed | <i>Rhizoctonia solani</i> AG-3PT is the major pathogen associated with potato stem canker and black scurf in Jordan |
| title_short | <i>Rhizoctonia solani</i> AG-3PT is the major pathogen associated with potato stem canker and black scurf in Jordan |
| title_sort | i rhizoctonia solani i ag 3pt is the major pathogen associated with potato stem canker and black scurf in jordan |
| topic | black scurf pathogenicity rhizoctonia solani stem canker thanatephorus cucumeris |
| url | https://www.aimspress.com/article/doi/10.3934/agrfood.2023006?viewType=HTML |
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