Impact of Aging on the Ovarian Extracellular Matrix and Derived 3D Scaffolds
Advances in medical care, improvements in sanitation, and rising living standards contribute to increased life expectancy. Although this reflects positive human development, it also poses new challenges. Among these, reproductive aging is gradually becoming a key health issue because the age of meno...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-01-01
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| Series: | Nanomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4991/12/3/345 |
| _version_ | 1797485932338741248 |
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| author | Georgia Pennarossa Teresina De Iorio Fulvio Gandolfi Tiziana A. L. Brevini |
| author_facet | Georgia Pennarossa Teresina De Iorio Fulvio Gandolfi Tiziana A. L. Brevini |
| author_sort | Georgia Pennarossa |
| collection | DOAJ |
| description | Advances in medical care, improvements in sanitation, and rising living standards contribute to increased life expectancy. Although this reflects positive human development, it also poses new challenges. Among these, reproductive aging is gradually becoming a key health issue because the age of menopause has remained constant at ~50 years, leading women to live longer in suboptimal endocrine conditions. An adequate understanding of ovarian senescence mechanisms is essential to prevent age-related diseases and to promote wellbeing, health, and longevity in women. We here analyze the impact of aging on the ovarian extracellular matrix (ECM), and we demonstrate significant changes in its composition and organization with collagen, glycosaminoglycans, and laminins significantly incremented, and elastin, as well as fibronectin, decreased. This is accompanied by a dynamic response in gene expression levels of the main ECM- and protease-related genes, indicating a direct impact of aging on the transcription machinery. Furthermore, in order to study the mechanisms driving aging and identify possible strategies to counteract ovarian tissue degeneration, we here described the successful production of a 3D ECM-based biological scaffold that preserves the structural modifications taking place in vivo and that represents a powerful high predictive in vitro model for reproductive aging and its prevention. |
| first_indexed | 2024-03-09T23:25:55Z |
| format | Article |
| id | doaj.art-fcdaaa22030848fb8e703f48d06fd48a |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-09T23:25:55Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-fcdaaa22030848fb8e703f48d06fd48a2023-11-23T17:19:15ZengMDPI AGNanomaterials2079-49912022-01-0112334510.3390/nano12030345Impact of Aging on the Ovarian Extracellular Matrix and Derived 3D ScaffoldsGeorgia Pennarossa0Teresina De Iorio1Fulvio Gandolfi2Tiziana A. L. Brevini3Laboratory of Biomedical Embryology, Department of Health, Animal Science and Food Safety and Center for Stem Cell Research, Università degli Studi di Milano, 20133 Milan, ItalyLaboratory of Biomedical Embryology, Department of Health, Animal Science and Food Safety and Center for Stem Cell Research, Università degli Studi di Milano, 20133 Milan, ItalyLaboratory of Biomedical Embryology, Department of Agricultural and Environmental Sciences—Production, Landscape, Agroenergy, Università degli Studi di Milano, 20133 Milan, ItalyLaboratory of Biomedical Embryology, Department of Health, Animal Science and Food Safety and Center for Stem Cell Research, Università degli Studi di Milano, 20133 Milan, ItalyAdvances in medical care, improvements in sanitation, and rising living standards contribute to increased life expectancy. Although this reflects positive human development, it also poses new challenges. Among these, reproductive aging is gradually becoming a key health issue because the age of menopause has remained constant at ~50 years, leading women to live longer in suboptimal endocrine conditions. An adequate understanding of ovarian senescence mechanisms is essential to prevent age-related diseases and to promote wellbeing, health, and longevity in women. We here analyze the impact of aging on the ovarian extracellular matrix (ECM), and we demonstrate significant changes in its composition and organization with collagen, glycosaminoglycans, and laminins significantly incremented, and elastin, as well as fibronectin, decreased. This is accompanied by a dynamic response in gene expression levels of the main ECM- and protease-related genes, indicating a direct impact of aging on the transcription machinery. Furthermore, in order to study the mechanisms driving aging and identify possible strategies to counteract ovarian tissue degeneration, we here described the successful production of a 3D ECM-based biological scaffold that preserves the structural modifications taking place in vivo and that represents a powerful high predictive in vitro model for reproductive aging and its prevention.https://www.mdpi.com/2079-4991/12/3/345reproductive agingsenescenceextracellular matrixwhole-ovary decellularizationECM-based bio-scaffoldporcine |
| spellingShingle | Georgia Pennarossa Teresina De Iorio Fulvio Gandolfi Tiziana A. L. Brevini Impact of Aging on the Ovarian Extracellular Matrix and Derived 3D Scaffolds Nanomaterials reproductive aging senescence extracellular matrix whole-ovary decellularization ECM-based bio-scaffold porcine |
| title | Impact of Aging on the Ovarian Extracellular Matrix and Derived 3D Scaffolds |
| title_full | Impact of Aging on the Ovarian Extracellular Matrix and Derived 3D Scaffolds |
| title_fullStr | Impact of Aging on the Ovarian Extracellular Matrix and Derived 3D Scaffolds |
| title_full_unstemmed | Impact of Aging on the Ovarian Extracellular Matrix and Derived 3D Scaffolds |
| title_short | Impact of Aging on the Ovarian Extracellular Matrix and Derived 3D Scaffolds |
| title_sort | impact of aging on the ovarian extracellular matrix and derived 3d scaffolds |
| topic | reproductive aging senescence extracellular matrix whole-ovary decellularization ECM-based bio-scaffold porcine |
| url | https://www.mdpi.com/2079-4991/12/3/345 |
| work_keys_str_mv | AT georgiapennarossa impactofagingontheovarianextracellularmatrixandderived3dscaffolds AT teresinadeiorio impactofagingontheovarianextracellularmatrixandderived3dscaffolds AT fulviogandolfi impactofagingontheovarianextracellularmatrixandderived3dscaffolds AT tizianaalbrevini impactofagingontheovarianextracellularmatrixandderived3dscaffolds |