Therapeutic Intervention for Various Hospital Setting Strains of Biofilm Forming <i>Candida auris</i> with Multiple Drug Resistance Mutations Using Nanomaterial Ag-Silicalite-1 Zeolite
<i>Candida auris</i> (<i>C. auris</i>), an emerging multidrug-resistant microorganism, with limited therapeutical options, is one of the leading causes of nosocomial infections. The current study includes 19 <i>C. auris</i> strains collected from King Fahd Hospita...
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MDPI AG
2022-10-01
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| Online Access: | https://www.mdpi.com/1999-4923/14/10/2251 |
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| author | Hanan A. Aldossary Suriya Rehman B. Rabindran Jermy Reem AlJindan Afra Aldayel Sayed AbdulAzeez Sultan Akhtar Firdos Alam Khan J. Francis Borgio Ebtesam Abdullah Al-Suhaimi |
| author_facet | Hanan A. Aldossary Suriya Rehman B. Rabindran Jermy Reem AlJindan Afra Aldayel Sayed AbdulAzeez Sultan Akhtar Firdos Alam Khan J. Francis Borgio Ebtesam Abdullah Al-Suhaimi |
| author_sort | Hanan A. Aldossary |
| collection | DOAJ |
| description | <i>Candida auris</i> (<i>C. auris</i>), an emerging multidrug-resistant microorganism, with limited therapeutical options, is one of the leading causes of nosocomial infections. The current study includes 19 <i>C. auris</i> strains collected from King Fahd Hospital of the University and King Fahad Specialist Hospital in Dammam, identified by <i>18S rRNA</i> gene and <i>ITS</i> region sequencing. Drug-resistance-associated mutations in <i>ERG11, TAC1B and FUR1</i> genes were screened to gain insight into the pattern of drug resistance. Molecular identification was successfully achieved using <i>18S rRNA</i> gene and <i>ITS</i> region and 5 drug-resistance-associated missense variants identified in the <i>ERG11</i> (F132Y and K143R) and <i>TAC1B</i> (H608Y, P611S and A640V) genes of <i>C. auris</i> strains, grouped into 3 clades. The prophylactic and therapeutic application of hydrothermally synthesized Ag-silicalite-1 (Si/Ag ratio 25) nanomaterial was tested against the 3 clades of clinical <i>C. auris</i> strains. 4wt%Ag/TiZSM-5 prepared using conventional impregnation technique was used for comparative study, and nano formulations were characterized using different techniques. The antibiofilm activity of nanomaterials was tested by cell kill assay, scanning electron microscopy (SEM) and light microscopy. Across all the clades of <i>C. auris</i> strains, 4 wt%Ag/TiZSM-5 and Ag-silicalite-1 demonstrated a significant (<i>p</i> = 1.1102 × 10<sup>−16</sup>) inhibitory effect on the biofilm’s survival rate: the lowest inhibition value was (10%) with Ag-silicalite-1 at 24 and 48 h incubation. A profound change in morphogenesis in addition to the reduction in the number of <i>C.</i><i>auris</i> cells was shown by SEM and light microscopy. The presence of a high surface area and the uniform dispersion of nanosized Ag species displays enhanced anti-<i>Candida</i> activity, and therefore it has great potential against the emerging multidrug-resistant <i>C. auris</i>. |
| first_indexed | 2024-03-09T19:35:10Z |
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| id | doaj.art-fcdc435731054cbc92adad3cedfe5169 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-09T19:35:10Z |
| publishDate | 2022-10-01 |
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| spelling | doaj.art-fcdc435731054cbc92adad3cedfe51692023-11-24T01:59:03ZengMDPI AGPharmaceutics1999-49232022-10-011410225110.3390/pharmaceutics14102251Therapeutic Intervention for Various Hospital Setting Strains of Biofilm Forming <i>Candida auris</i> with Multiple Drug Resistance Mutations Using Nanomaterial Ag-Silicalite-1 ZeoliteHanan A. Aldossary0Suriya Rehman1B. Rabindran Jermy2Reem AlJindan3Afra Aldayel4Sayed AbdulAzeez5Sultan Akhtar6Firdos Alam Khan7J. Francis Borgio8Ebtesam Abdullah Al-Suhaimi9Master Program of Biotechnology, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi ArabiaDepartment of Epidemic Diseases Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi ArabiaDepartment of Nano-Medicine Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi ArabiaDepartment of Microbiology, College of Medicine, Imam Abdulrahman Bin Faisal University, Dammam 40017, Saudi ArabiaDepartment of Pathology & Lab Medicine, King Fahad Specialist Hospital, Dammam 32253, Saudi ArabiaDepartment of Genetic Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi ArabiaDepartment of Biophysics Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi ArabiaDepartment of Stem Cell Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi ArabiaDepartment of Epidemic Diseases Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi ArabiaInstitute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi Arabia<i>Candida auris</i> (<i>C. auris</i>), an emerging multidrug-resistant microorganism, with limited therapeutical options, is one of the leading causes of nosocomial infections. The current study includes 19 <i>C. auris</i> strains collected from King Fahd Hospital of the University and King Fahad Specialist Hospital in Dammam, identified by <i>18S rRNA</i> gene and <i>ITS</i> region sequencing. Drug-resistance-associated mutations in <i>ERG11, TAC1B and FUR1</i> genes were screened to gain insight into the pattern of drug resistance. Molecular identification was successfully achieved using <i>18S rRNA</i> gene and <i>ITS</i> region and 5 drug-resistance-associated missense variants identified in the <i>ERG11</i> (F132Y and K143R) and <i>TAC1B</i> (H608Y, P611S and A640V) genes of <i>C. auris</i> strains, grouped into 3 clades. The prophylactic and therapeutic application of hydrothermally synthesized Ag-silicalite-1 (Si/Ag ratio 25) nanomaterial was tested against the 3 clades of clinical <i>C. auris</i> strains. 4wt%Ag/TiZSM-5 prepared using conventional impregnation technique was used for comparative study, and nano formulations were characterized using different techniques. The antibiofilm activity of nanomaterials was tested by cell kill assay, scanning electron microscopy (SEM) and light microscopy. Across all the clades of <i>C. auris</i> strains, 4 wt%Ag/TiZSM-5 and Ag-silicalite-1 demonstrated a significant (<i>p</i> = 1.1102 × 10<sup>−16</sup>) inhibitory effect on the biofilm’s survival rate: the lowest inhibition value was (10%) with Ag-silicalite-1 at 24 and 48 h incubation. A profound change in morphogenesis in addition to the reduction in the number of <i>C.</i><i>auris</i> cells was shown by SEM and light microscopy. The presence of a high surface area and the uniform dispersion of nanosized Ag species displays enhanced anti-<i>Candida</i> activity, and therefore it has great potential against the emerging multidrug-resistant <i>C. auris</i>.https://www.mdpi.com/1999-4923/14/10/2251<i>Candida auris</i>biofilmhydrothermalnanomaterialAg-silicalite-1therapeutics |
| spellingShingle | Hanan A. Aldossary Suriya Rehman B. Rabindran Jermy Reem AlJindan Afra Aldayel Sayed AbdulAzeez Sultan Akhtar Firdos Alam Khan J. Francis Borgio Ebtesam Abdullah Al-Suhaimi Therapeutic Intervention for Various Hospital Setting Strains of Biofilm Forming <i>Candida auris</i> with Multiple Drug Resistance Mutations Using Nanomaterial Ag-Silicalite-1 Zeolite Pharmaceutics <i>Candida auris</i> biofilm hydrothermal nanomaterial Ag-silicalite-1 therapeutics |
| title | Therapeutic Intervention for Various Hospital Setting Strains of Biofilm Forming <i>Candida auris</i> with Multiple Drug Resistance Mutations Using Nanomaterial Ag-Silicalite-1 Zeolite |
| title_full | Therapeutic Intervention for Various Hospital Setting Strains of Biofilm Forming <i>Candida auris</i> with Multiple Drug Resistance Mutations Using Nanomaterial Ag-Silicalite-1 Zeolite |
| title_fullStr | Therapeutic Intervention for Various Hospital Setting Strains of Biofilm Forming <i>Candida auris</i> with Multiple Drug Resistance Mutations Using Nanomaterial Ag-Silicalite-1 Zeolite |
| title_full_unstemmed | Therapeutic Intervention for Various Hospital Setting Strains of Biofilm Forming <i>Candida auris</i> with Multiple Drug Resistance Mutations Using Nanomaterial Ag-Silicalite-1 Zeolite |
| title_short | Therapeutic Intervention for Various Hospital Setting Strains of Biofilm Forming <i>Candida auris</i> with Multiple Drug Resistance Mutations Using Nanomaterial Ag-Silicalite-1 Zeolite |
| title_sort | therapeutic intervention for various hospital setting strains of biofilm forming i candida auris i with multiple drug resistance mutations using nanomaterial ag silicalite 1 zeolite |
| topic | <i>Candida auris</i> biofilm hydrothermal nanomaterial Ag-silicalite-1 therapeutics |
| url | https://www.mdpi.com/1999-4923/14/10/2251 |
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