The development of Alternaria alternata is prevented by chitinases and ß-1,3- glucanases from Citrus limon seedlings
In addition to phytoalexin synthesis, the defense response of intact Citrus limon seedlings against Alternaria alternata involves both constitutive and induced enzyme activities such as chitinases (Ch) and ß-1,3-glucanases (Glu). A. alternata conidial germination was prevented by protein extracts fr...
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| Format: | Article |
| Language: | English |
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BMC
2003-01-01
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| Series: | Biological Research |
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| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300012 |
| _version_ | 1818340481068695552 |
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| author | NORA FANTA XIMENA ORTEGA LUZ M PÉREZ |
| author_facet | NORA FANTA XIMENA ORTEGA LUZ M PÉREZ |
| author_sort | NORA FANTA |
| collection | DOAJ |
| description | In addition to phytoalexin synthesis, the defense response of intact Citrus limon seedlings against Alternaria alternata involves both constitutive and induced enzyme activities such as chitinases (Ch) and ß-1,3-glucanases (Glu). A. alternata conidial germination was prevented by protein extracts from inoculated lemon seedlings, but also by extracts from mock-inoculated specimens. On the other hand, degradation of mycelia was accomplished only by protein extracts from inoculated seedlings. The presence of six Ch isoenzymes and of four Glu isoenzymes was detected in protein extracts from mock-inoculated seedlings. As a result of fungal inoculation, the isoenzyme pattern of Ch and Glu changed, making possible the detection of a new Ch isoenzyme and of three new Glu. Also, some constitutive Ch and Glu increased their enzyme activity, and those Ch that increased their activy also showed a broadening of their substrate specificity. These changes were prevented by a-amanitin and cycloheximide, suggesting that the presence of new Ch and Glu isoenzymes was due to de novo synthesis of proteins. Results suggest that constitutive Ch and Glu could act as pre-formed defense molecules in Citrus limon preventing A. alternata germination, while those induced after fungal inoculation of lemon seedlings could act along with the former, to produce lysis of fungal mycelia, resulting in a more efficient control of A. alternata development.. |
| first_indexed | 2024-12-13T15:43:35Z |
| format | Article |
| id | doaj.art-fcdcedc96a0f442e9dd9c618aa6251ee |
| institution | Directory Open Access Journal |
| issn | 0716-9760 0717-6287 |
| language | English |
| last_indexed | 2024-12-13T15:43:35Z |
| publishDate | 2003-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Research |
| spelling | doaj.art-fcdcedc96a0f442e9dd9c618aa6251ee2022-12-21T23:39:47ZengBMCBiological Research0716-97600717-62872003-01-01363-4411420The development of Alternaria alternata is prevented by chitinases and ß-1,3- glucanases from Citrus limon seedlingsNORA FANTAXIMENA ORTEGALUZ M PÉREZIn addition to phytoalexin synthesis, the defense response of intact Citrus limon seedlings against Alternaria alternata involves both constitutive and induced enzyme activities such as chitinases (Ch) and ß-1,3-glucanases (Glu). A. alternata conidial germination was prevented by protein extracts from inoculated lemon seedlings, but also by extracts from mock-inoculated specimens. On the other hand, degradation of mycelia was accomplished only by protein extracts from inoculated seedlings. The presence of six Ch isoenzymes and of four Glu isoenzymes was detected in protein extracts from mock-inoculated seedlings. As a result of fungal inoculation, the isoenzyme pattern of Ch and Glu changed, making possible the detection of a new Ch isoenzyme and of three new Glu. Also, some constitutive Ch and Glu increased their enzyme activity, and those Ch that increased their activy also showed a broadening of their substrate specificity. These changes were prevented by a-amanitin and cycloheximide, suggesting that the presence of new Ch and Glu isoenzymes was due to de novo synthesis of proteins. Results suggest that constitutive Ch and Glu could act as pre-formed defense molecules in Citrus limon preventing A. alternata germination, while those induced after fungal inoculation of lemon seedlings could act along with the former, to produce lysis of fungal mycelia, resulting in a more efficient control of A. alternata development..http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300012Alternaria alternatalemonhypersensitive responseChitinasesGlucanases |
| spellingShingle | NORA FANTA XIMENA ORTEGA LUZ M PÉREZ The development of Alternaria alternata is prevented by chitinases and ß-1,3- glucanases from Citrus limon seedlings Biological Research Alternaria alternata lemon hypersensitive response Chitinases Glucanases |
| title | The development of Alternaria alternata is prevented by chitinases and ß-1,3- glucanases from Citrus limon seedlings |
| title_full | The development of Alternaria alternata is prevented by chitinases and ß-1,3- glucanases from Citrus limon seedlings |
| title_fullStr | The development of Alternaria alternata is prevented by chitinases and ß-1,3- glucanases from Citrus limon seedlings |
| title_full_unstemmed | The development of Alternaria alternata is prevented by chitinases and ß-1,3- glucanases from Citrus limon seedlings |
| title_short | The development of Alternaria alternata is prevented by chitinases and ß-1,3- glucanases from Citrus limon seedlings |
| title_sort | development of alternaria alternata is prevented by chitinases and ss 1 3 glucanases from citrus limon seedlings |
| topic | Alternaria alternata lemon hypersensitive response Chitinases Glucanases |
| url | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300012 |
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