Autologous T cells expressing the oncogenic transcription factor KLF6-SV1 prevent apoptosis of chronic lymphocytic leukemia cells.
Crosstalk between leukemic cells and the tumor microenvironment is of importance in chronic lymphocytic leukemia (CLL). T cells seem to sustain the survival of CLL cells by various mechanisms. The Krüppel-like family of transcription factors (KLFs) are identified as regulators of proliferation and c...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5809069?pdf=render |
| _version_ | 1818042034125012992 |
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| author | Parviz Kokhaei Mohammad Hojjat-Farsangi Fariba Mozaffari Ali Moshfegh Fatemeh Pak Ali Rashidy-Pour Marzia Palma Lotta Hansson Anders Österborg Håkan Mellstedt |
| author_facet | Parviz Kokhaei Mohammad Hojjat-Farsangi Fariba Mozaffari Ali Moshfegh Fatemeh Pak Ali Rashidy-Pour Marzia Palma Lotta Hansson Anders Österborg Håkan Mellstedt |
| author_sort | Parviz Kokhaei |
| collection | DOAJ |
| description | Crosstalk between leukemic cells and the tumor microenvironment is of importance in chronic lymphocytic leukemia (CLL). T cells seem to sustain the survival of CLL cells by various mechanisms. The Krüppel-like family of transcription factors (KLFs) are identified as regulators of proliferation and cell death. In the present study, we analyzed the expression of the wild type (WT) gene KLF6 and the oncogenic splice variant 1 (KLF6-SV1) at the mRNA level in subsets of T cells from CLL patients (n = 29), multiple myeloma patients (n = 6) and normal donors (n = 10). RNA Silencing was used for wtKLF6 and KLF6-SV1. Tumor cell apoptosis was measured. A significant overexpression of wtKLF6 and KLF6-SV1 in T cells of CLL patients compared to normal donors and myeloma patients was noted (p<0.002). Western blot showed that both wtKLF6 and KLF6-SV1 were expressed in purified T cells from CLL patients. KLF6-SV1 siRNA transfection induced a significant down-regulation of KLF6-SV1 in CLL T cells, which lost the capability to sustain the growth of leukemic cells. However, no such a significant effect was seen after wtKLF6 transfection of the autologous T cells. The results suggest that KLF6-SV1 may play a role in the regulation of survival CLL cells. |
| first_indexed | 2024-12-10T08:39:54Z |
| format | Article |
| id | doaj.art-fcdf895cba0b43c5a53915ce617f4a25 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T08:39:54Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-fcdf895cba0b43c5a53915ce617f4a252022-12-22T01:55:52ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01132e019283910.1371/journal.pone.0192839Autologous T cells expressing the oncogenic transcription factor KLF6-SV1 prevent apoptosis of chronic lymphocytic leukemia cells.Parviz KokhaeiMohammad Hojjat-FarsangiFariba MozaffariAli MoshfeghFatemeh PakAli Rashidy-PourMarzia PalmaLotta HanssonAnders ÖsterborgHåkan MellstedtCrosstalk between leukemic cells and the tumor microenvironment is of importance in chronic lymphocytic leukemia (CLL). T cells seem to sustain the survival of CLL cells by various mechanisms. The Krüppel-like family of transcription factors (KLFs) are identified as regulators of proliferation and cell death. In the present study, we analyzed the expression of the wild type (WT) gene KLF6 and the oncogenic splice variant 1 (KLF6-SV1) at the mRNA level in subsets of T cells from CLL patients (n = 29), multiple myeloma patients (n = 6) and normal donors (n = 10). RNA Silencing was used for wtKLF6 and KLF6-SV1. Tumor cell apoptosis was measured. A significant overexpression of wtKLF6 and KLF6-SV1 in T cells of CLL patients compared to normal donors and myeloma patients was noted (p<0.002). Western blot showed that both wtKLF6 and KLF6-SV1 were expressed in purified T cells from CLL patients. KLF6-SV1 siRNA transfection induced a significant down-regulation of KLF6-SV1 in CLL T cells, which lost the capability to sustain the growth of leukemic cells. However, no such a significant effect was seen after wtKLF6 transfection of the autologous T cells. The results suggest that KLF6-SV1 may play a role in the regulation of survival CLL cells.http://europepmc.org/articles/PMC5809069?pdf=render |
| spellingShingle | Parviz Kokhaei Mohammad Hojjat-Farsangi Fariba Mozaffari Ali Moshfegh Fatemeh Pak Ali Rashidy-Pour Marzia Palma Lotta Hansson Anders Österborg Håkan Mellstedt Autologous T cells expressing the oncogenic transcription factor KLF6-SV1 prevent apoptosis of chronic lymphocytic leukemia cells. PLoS ONE |
| title | Autologous T cells expressing the oncogenic transcription factor KLF6-SV1 prevent apoptosis of chronic lymphocytic leukemia cells. |
| title_full | Autologous T cells expressing the oncogenic transcription factor KLF6-SV1 prevent apoptosis of chronic lymphocytic leukemia cells. |
| title_fullStr | Autologous T cells expressing the oncogenic transcription factor KLF6-SV1 prevent apoptosis of chronic lymphocytic leukemia cells. |
| title_full_unstemmed | Autologous T cells expressing the oncogenic transcription factor KLF6-SV1 prevent apoptosis of chronic lymphocytic leukemia cells. |
| title_short | Autologous T cells expressing the oncogenic transcription factor KLF6-SV1 prevent apoptosis of chronic lymphocytic leukemia cells. |
| title_sort | autologous t cells expressing the oncogenic transcription factor klf6 sv1 prevent apoptosis of chronic lymphocytic leukemia cells |
| url | http://europepmc.org/articles/PMC5809069?pdf=render |
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