Determination of Aflatoxins in Feed by HPLC and PCR
The aim of this study is to identify aflatoxin secretion isolates in animal feeds by using HPLC and PCR methods . In this study we collected fourty three samples of animal feed from different sites in Iraq (maize ,soybean ,sunflower grain ,barley grain, wheat). we isolated fungi on potato dextrose...
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| Format: | Article |
| Language: | English |
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LPPT Universitas Gadjah Mada
2020-11-01
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| Series: | Journal of Food and Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | https://jurnal.ugm.ac.id/v3/JFPS/article/view/881 |
| _version_ | 1797449116150661120 |
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| author | Areej Zuhair Azeez Mrwa Thamer Hindi Maha Muhamed Khudiar Adel Saadi AL Saadi Noor Ibrahim Khadim |
| author_facet | Areej Zuhair Azeez Mrwa Thamer Hindi Maha Muhamed Khudiar Adel Saadi AL Saadi Noor Ibrahim Khadim |
| author_sort | Areej Zuhair Azeez |
| collection | DOAJ |
| description | The aim of this study is to identify aflatoxin secretion isolates in animal feeds by using HPLC and PCR methods . In this study we collected fourty three samples of animal feed from different sites in Iraq (maize ,soybean ,sunflower grain ,barley grain, wheat). we isolated fungi on potato dextrose agar, Aspergillus flavus fungi was isolated from this samples and identified the enzyme activities were tested for this isolate. The detection and determination for aflatoxin secretion of the isolates were done by using High Performance Liquid Chromatography (HPLC) technique. Twelve isolates shown Aflatoxin B1 secretion. Polymrease chain reaction ( PCR) technique is an alternative method to detect for Aspergillus spp. strains that secret aflatoxin by using specific primers( ITS1) endogenous gene for Aspergillus flavus and (ord , nor) genes for aflatoxin B1 secreation, the PCR technique considered to be an important role for safety and quality in industrial food and feed. |
| first_indexed | 2024-03-09T14:20:12Z |
| format | Article |
| id | doaj.art-fce1235c95ba49acbd4b0fe9ee2d7e98 |
| institution | Directory Open Access Journal |
| issn | 2089-7200 2339-0948 |
| language | English |
| last_indexed | 2024-03-09T14:20:12Z |
| publishDate | 2020-11-01 |
| publisher | LPPT Universitas Gadjah Mada |
| record_format | Article |
| series | Journal of Food and Pharmaceutical Sciences |
| spelling | doaj.art-fce1235c95ba49acbd4b0fe9ee2d7e982023-11-28T15:20:26ZengLPPT Universitas Gadjah MadaJournal of Food and Pharmaceutical Sciences2089-72002339-09482020-11-0131532310.22146/jfps.881881Determination of Aflatoxins in Feed by HPLC and PCRAreej Zuhair Azeez0Mrwa Thamer Hindi1Maha Muhamed Khudiar2Adel Saadi AL Saadi3Noor Ibrahim Khadim4Environment and Water Directorate, Food Contamination Research Center. Ministry of Science and Technology-Environment .Iraq -BaghdadEnvironment and Water Directorate, Food Contamination Research Center, Ministry of Science and Technology-Environment, Iraq, BaghdadEnvironment and Water Directorate, Food Contamination Research Center, Ministry of Science and Technology-Environment, Iraq, BaghdadEnvironment and Water Directorate, Food Contamination Research Center, Ministry of Science and Technology-Environment, Iraq, BaghdadEnvironment and Water Directorate, Food Contamination Research Center, Ministry of Science and Technology-Environment, Iraq, BaghdadThe aim of this study is to identify aflatoxin secretion isolates in animal feeds by using HPLC and PCR methods . In this study we collected fourty three samples of animal feed from different sites in Iraq (maize ,soybean ,sunflower grain ,barley grain, wheat). we isolated fungi on potato dextrose agar, Aspergillus flavus fungi was isolated from this samples and identified the enzyme activities were tested for this isolate. The detection and determination for aflatoxin secretion of the isolates were done by using High Performance Liquid Chromatography (HPLC) technique. Twelve isolates shown Aflatoxin B1 secretion. Polymrease chain reaction ( PCR) technique is an alternative method to detect for Aspergillus spp. strains that secret aflatoxin by using specific primers( ITS1) endogenous gene for Aspergillus flavus and (ord , nor) genes for aflatoxin B1 secreation, the PCR technique considered to be an important role for safety and quality in industrial food and feed.https://jurnal.ugm.ac.id/v3/JFPS/article/view/881aflatoxinaspergillus flavushplcpcrtoxicity |
| spellingShingle | Areej Zuhair Azeez Mrwa Thamer Hindi Maha Muhamed Khudiar Adel Saadi AL Saadi Noor Ibrahim Khadim Determination of Aflatoxins in Feed by HPLC and PCR Journal of Food and Pharmaceutical Sciences aflatoxin aspergillus flavus hplc pcr toxicity |
| title | Determination of Aflatoxins in Feed by HPLC and PCR |
| title_full | Determination of Aflatoxins in Feed by HPLC and PCR |
| title_fullStr | Determination of Aflatoxins in Feed by HPLC and PCR |
| title_full_unstemmed | Determination of Aflatoxins in Feed by HPLC and PCR |
| title_short | Determination of Aflatoxins in Feed by HPLC and PCR |
| title_sort | determination of aflatoxins in feed by hplc and pcr |
| topic | aflatoxin aspergillus flavus hplc pcr toxicity |
| url | https://jurnal.ugm.ac.id/v3/JFPS/article/view/881 |
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