Decellularized Human Umbilical Artery Used as Nerve Conduit
Treatment of injuries to peripheral nerves after a segmental defect is one of the most challenging surgical problems. Despite advancements in microsurgical techniques, complete recovery of nerve function after repair has not been achieved. The purpose of this study was to evaluate the use of the dec...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2018-11-01
|
| Series: | Bioengineering |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2306-5354/5/4/100 |
| _version_ | 1797706692429873152 |
|---|---|
| author | Ioanna Gontika Michalis Katsimpoulas Efstathios Antoniou Alkiviadis Kostakis Catherine Stavropoulos-Giokas Efstathios Michalopoulos |
| author_facet | Ioanna Gontika Michalis Katsimpoulas Efstathios Antoniou Alkiviadis Kostakis Catherine Stavropoulos-Giokas Efstathios Michalopoulos |
| author_sort | Ioanna Gontika |
| collection | DOAJ |
| description | Treatment of injuries to peripheral nerves after a segmental defect is one of the most challenging surgical problems. Despite advancements in microsurgical techniques, complete recovery of nerve function after repair has not been achieved. The purpose of this study was to evaluate the use of the decellularized human umbilical artery (hUA) as nerve guidance conduit. A segmental peripheral nerve injury was created in 24 Sprague⁻Dawley rats. The animals were organized into two experimental groups with different forms of repair: decellularized hUA (<i>n</i> = 12), and autologous nerve graft (<i>n</i> = 12). Sciatic faction index and gastrocnemius muscle values were calculated for functional recovery evaluation. Nerve morphometry was used to analyze nerve regeneration. Results showed that decellularized hUAs after implantation were rich in nerve fibers and characterized by improved Sciatic Functional index (SFI) values. Decellularized hUA may support elongation and bridging of the 10 mm nerve gap. |
| first_indexed | 2024-03-12T05:56:04Z |
| format | Article |
| id | doaj.art-fce12a232f354e2bb6ce1f5abb63fb20 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-12T05:56:04Z |
| publishDate | 2018-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-fce12a232f354e2bb6ce1f5abb63fb202023-09-03T04:39:39ZengMDPI AGBioengineering2306-53542018-11-015410010.3390/bioengineering5040100bioengineering5040100Decellularized Human Umbilical Artery Used as Nerve ConduitIoanna Gontika0Michalis Katsimpoulas1Efstathios Antoniou2Alkiviadis Kostakis3Catherine Stavropoulos-Giokas4Efstathios Michalopoulos5Hellenic Cord Blood Bank, Biomedical Research Foudation Academy of Athens, 4 Soranou Ephessiou Street, 11527 Athens, GreeceCenter of Clinical, Experimental Surgery and Translational Research, Biomedical Research Foundation of the Academy of Athens, 4 Soranou Ephessiou Street, 11527 Athens, GreeceSecond Department of Propaedeutic Surgery, University of Athens, Medical School, “Laiko” General Hospital 17 Agios Thomas Street, 11527 Athens, GreeceCenter of Clinical, Experimental Surgery and Translational Research, Biomedical Research Foundation of the Academy of Athens, 4 Soranou Ephessiou Street, 11527 Athens, GreeceCenter of Clinical, Experimental Surgery and Translational Research, Biomedical Research Foundation of the Academy of Athens, 4 Soranou Ephessiou Street, 11527 Athens, GreeceHellenic Cord Blood Bank, Biomedical Research Foudation Academy of Athens, 4 Soranou Ephessiou Street, 11527 Athens, GreeceTreatment of injuries to peripheral nerves after a segmental defect is one of the most challenging surgical problems. Despite advancements in microsurgical techniques, complete recovery of nerve function after repair has not been achieved. The purpose of this study was to evaluate the use of the decellularized human umbilical artery (hUA) as nerve guidance conduit. A segmental peripheral nerve injury was created in 24 Sprague⁻Dawley rats. The animals were organized into two experimental groups with different forms of repair: decellularized hUA (<i>n</i> = 12), and autologous nerve graft (<i>n</i> = 12). Sciatic faction index and gastrocnemius muscle values were calculated for functional recovery evaluation. Nerve morphometry was used to analyze nerve regeneration. Results showed that decellularized hUAs after implantation were rich in nerve fibers and characterized by improved Sciatic Functional index (SFI) values. Decellularized hUA may support elongation and bridging of the 10 mm nerve gap.https://www.mdpi.com/2306-5354/5/4/100umbilical arteriesnerve regenerationnerve conduit |
| spellingShingle | Ioanna Gontika Michalis Katsimpoulas Efstathios Antoniou Alkiviadis Kostakis Catherine Stavropoulos-Giokas Efstathios Michalopoulos Decellularized Human Umbilical Artery Used as Nerve Conduit Bioengineering umbilical arteries nerve regeneration nerve conduit |
| title | Decellularized Human Umbilical Artery Used as Nerve Conduit |
| title_full | Decellularized Human Umbilical Artery Used as Nerve Conduit |
| title_fullStr | Decellularized Human Umbilical Artery Used as Nerve Conduit |
| title_full_unstemmed | Decellularized Human Umbilical Artery Used as Nerve Conduit |
| title_short | Decellularized Human Umbilical Artery Used as Nerve Conduit |
| title_sort | decellularized human umbilical artery used as nerve conduit |
| topic | umbilical arteries nerve regeneration nerve conduit |
| url | https://www.mdpi.com/2306-5354/5/4/100 |
| work_keys_str_mv | AT ioannagontika decellularizedhumanumbilicalarteryusedasnerveconduit AT michaliskatsimpoulas decellularizedhumanumbilicalarteryusedasnerveconduit AT efstathiosantoniou decellularizedhumanumbilicalarteryusedasnerveconduit AT alkiviadiskostakis decellularizedhumanumbilicalarteryusedasnerveconduit AT catherinestavropoulosgiokas decellularizedhumanumbilicalarteryusedasnerveconduit AT efstathiosmichalopoulos decellularizedhumanumbilicalarteryusedasnerveconduit |