Micropatterning of Cells on Gold Surfaces for Biophysical Applications
Summary: We developed a reproducible micropatterning method to manipulate and normalize cell shape and cell-cell separation on gold. We used methoxy polyethylene glycol thiol (PEG-SH) to create a self-assembled monolayer that can be oxidized at desired shapes through a photomask with deep UV light....
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-12-01
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| Series: | STAR Protocols |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166720300939 |
| _version_ | 1819295881001697280 |
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| author | Carolin Grandy Peter Kolb Fabian Port Kay-Eberhard Gottschalk |
| author_facet | Carolin Grandy Peter Kolb Fabian Port Kay-Eberhard Gottschalk |
| author_sort | Carolin Grandy |
| collection | DOAJ |
| description | Summary: We developed a reproducible micropatterning method to manipulate and normalize cell shape and cell-cell separation on gold. We used methoxy polyethylene glycol thiol (PEG-SH) to create a self-assembled monolayer that can be oxidized at desired shapes through a photomask with deep UV light. The oxidized PEG can be coated with extracellular matrix proteins and seeded with cells adopting the pre-defined shape. The developed and analyzed surfaces can be used in a wide range of biophysical applications. |
| first_indexed | 2024-12-24T04:49:15Z |
| format | Article |
| id | doaj.art-fce2468ec26c420b93da2ea65cb5e383 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-24T04:49:15Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-fce2468ec26c420b93da2ea65cb5e3832022-12-21T17:14:36ZengElsevierSTAR Protocols2666-16672020-12-0113100106Micropatterning of Cells on Gold Surfaces for Biophysical ApplicationsCarolin Grandy0Peter Kolb1Fabian Port2Kay-Eberhard Gottschalk3University Ulm, Institute of Experimental Physics, Ulm, Baden-Württemberg 89081, GermanyUniversity Ulm, Institute of Experimental Physics, Ulm, Baden-Württemberg 89081, GermanyUniversity Ulm, Institute of Experimental Physics, Ulm, Baden-Württemberg 89081, GermanyUniversity Ulm, Institute of Experimental Physics, Ulm, Baden-Württemberg 89081, Germany; Corresponding authorSummary: We developed a reproducible micropatterning method to manipulate and normalize cell shape and cell-cell separation on gold. We used methoxy polyethylene glycol thiol (PEG-SH) to create a self-assembled monolayer that can be oxidized at desired shapes through a photomask with deep UV light. The oxidized PEG can be coated with extracellular matrix proteins and seeded with cells adopting the pre-defined shape. The developed and analyzed surfaces can be used in a wide range of biophysical applications.http://www.sciencedirect.com/science/article/pii/S2666166720300939 |
| spellingShingle | Carolin Grandy Peter Kolb Fabian Port Kay-Eberhard Gottschalk Micropatterning of Cells on Gold Surfaces for Biophysical Applications STAR Protocols |
| title | Micropatterning of Cells on Gold Surfaces for Biophysical Applications |
| title_full | Micropatterning of Cells on Gold Surfaces for Biophysical Applications |
| title_fullStr | Micropatterning of Cells on Gold Surfaces for Biophysical Applications |
| title_full_unstemmed | Micropatterning of Cells on Gold Surfaces for Biophysical Applications |
| title_short | Micropatterning of Cells on Gold Surfaces for Biophysical Applications |
| title_sort | micropatterning of cells on gold surfaces for biophysical applications |
| url | http://www.sciencedirect.com/science/article/pii/S2666166720300939 |
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