Anatomomopathological and immunohistochemical analyses of the spleen and lymph node of dogs seropositives for leishmaniasis in serological tests
Canine leishmaniasis (CanL) is a zoonosis caused by the protozoan of the species Leishmania infantum. The spleen and lymph nodes undergo morphological changes during CanL. This research aimed to perform an anatomopathological and immunohistochemical study of these organs in dogs reactive to leishma...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Goiás
2021-08-01
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| Series: | Ciência Animal Brasileira |
| Online Access: | https://revistas.ufg.br/vet/article/view/68909 |
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| author | Aline Oliveira de Magalhães Lucilandia Maria Bezerra Diego Pereira Araújo Bruna Siqueira Gomes de Lima Leandro do Padro Assunção Eduardo de Paula Nascente Ana Paula Iglesias Santin Rodrigo Caldas Menezes Veridiana Maria Brianezi Dignani de Moura |
| author_facet | Aline Oliveira de Magalhães Lucilandia Maria Bezerra Diego Pereira Araújo Bruna Siqueira Gomes de Lima Leandro do Padro Assunção Eduardo de Paula Nascente Ana Paula Iglesias Santin Rodrigo Caldas Menezes Veridiana Maria Brianezi Dignani de Moura |
| author_sort | Aline Oliveira de Magalhães |
| collection | DOAJ |
| description |
Canine leishmaniasis (CanL) is a zoonosis caused by the protozoan of the species Leishmania infantum. The spleen and lymph nodes undergo morphological changes during CanL. This research aimed to perform an anatomopathological and immunohistochemical study of these organs in dogs reactive to leishmaniasis in the Dual-path Platform chromatographic immunoassay (DPP®) and Enzyme Immunoabsorption Assay (ELISA). Twenty-seven dogs were evaluated for anatomopathological examination with 92.6% having clinical signs. Splenomegaly and lymphadenomegaly were the main macroscopic changes. All dogs showed changes in the spleen, unrelated to the parasitic load, with granulomatous splenitis being the most severe change. Diffuse cortical and paracortical hyperplasia and spinal cord hyperplasia and hypertrophy were observed in the lymph nodes. Amastigote forms of Leishmania spp. were found in the spleen and lymph node during histopathological and immunohistochemical evaluations, with good agreement between these evaluations (k = 0.55, p = 0.00124), but no difference was observed in the parasitic intensity of these organs (p = 0.23) . Thus, the spleen and lymph node reactive to leishmaniasis on the DPP® and ELISA tests show histomorphological changes resulting from the disease. In addition, the spleen and lymph node have a parasitic load similar to those found by direct diagnostic methods.
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| first_indexed | 2024-03-13T02:42:58Z |
| format | Article |
| id | doaj.art-fce269fbebce42ce9650ed293faa96b0 |
| institution | Directory Open Access Journal |
| issn | 1518-2797 1809-6891 |
| language | English |
| last_indexed | 2024-03-13T02:42:58Z |
| publishDate | 2021-08-01 |
| publisher | Universidade Federal de Goiás |
| record_format | Article |
| series | Ciência Animal Brasileira |
| spelling | doaj.art-fce269fbebce42ce9650ed293faa96b02023-06-28T21:07:18ZengUniversidade Federal de GoiásCiência Animal Brasileira1518-27971809-68912021-08-0122Anatomomopathological and immunohistochemical analyses of the spleen and lymph node of dogs seropositives for leishmaniasis in serological testsAline Oliveira de Magalhães0Lucilandia Maria Bezerra1Diego Pereira Araújo2Bruna Siqueira Gomes de Lima3Leandro do Padro Assunção4Eduardo de Paula Nascente5Ana Paula Iglesias Santin6Rodrigo Caldas Menezes7Veridiana Maria Brianezi Dignani de Moura8Universidade Federal de Goiás (UFG), Goiânia, GO, BrasilUniversidade Federal de Goiás (UFG), Goiânia, GO, BrasilUniversidade Federal de Goiás (UFG), Goiânia, GO, BrasilUniversidade Federal de Goiás (UFG), Goiânia, GO, BrasilUniversidade Federal de Goiás (UFG), Goiânia, GO, BrasilUniversidade Federal de Goiás (UFG), Goiânia, GO, BrasilUniversidade Federal de Goiás (UFG), Goiânia, GO, BrasilFundação Oswaldo Cruz (Fiocruz), Rio de Janeiro, RJ, Brasil.Universidade Federal de Goiás (UFG), Goiânia, GO, Brasil Canine leishmaniasis (CanL) is a zoonosis caused by the protozoan of the species Leishmania infantum. The spleen and lymph nodes undergo morphological changes during CanL. This research aimed to perform an anatomopathological and immunohistochemical study of these organs in dogs reactive to leishmaniasis in the Dual-path Platform chromatographic immunoassay (DPP®) and Enzyme Immunoabsorption Assay (ELISA). Twenty-seven dogs were evaluated for anatomopathological examination with 92.6% having clinical signs. Splenomegaly and lymphadenomegaly were the main macroscopic changes. All dogs showed changes in the spleen, unrelated to the parasitic load, with granulomatous splenitis being the most severe change. Diffuse cortical and paracortical hyperplasia and spinal cord hyperplasia and hypertrophy were observed in the lymph nodes. Amastigote forms of Leishmania spp. were found in the spleen and lymph node during histopathological and immunohistochemical evaluations, with good agreement between these evaluations (k = 0.55, p = 0.00124), but no difference was observed in the parasitic intensity of these organs (p = 0.23) . Thus, the spleen and lymph node reactive to leishmaniasis on the DPP® and ELISA tests show histomorphological changes resulting from the disease. In addition, the spleen and lymph node have a parasitic load similar to those found by direct diagnostic methods. https://revistas.ufg.br/vet/article/view/68909 |
| spellingShingle | Aline Oliveira de Magalhães Lucilandia Maria Bezerra Diego Pereira Araújo Bruna Siqueira Gomes de Lima Leandro do Padro Assunção Eduardo de Paula Nascente Ana Paula Iglesias Santin Rodrigo Caldas Menezes Veridiana Maria Brianezi Dignani de Moura Anatomomopathological and immunohistochemical analyses of the spleen and lymph node of dogs seropositives for leishmaniasis in serological tests Ciência Animal Brasileira |
| title | Anatomomopathological and immunohistochemical analyses of the spleen and lymph node of dogs seropositives for leishmaniasis in serological tests |
| title_full | Anatomomopathological and immunohistochemical analyses of the spleen and lymph node of dogs seropositives for leishmaniasis in serological tests |
| title_fullStr | Anatomomopathological and immunohistochemical analyses of the spleen and lymph node of dogs seropositives for leishmaniasis in serological tests |
| title_full_unstemmed | Anatomomopathological and immunohistochemical analyses of the spleen and lymph node of dogs seropositives for leishmaniasis in serological tests |
| title_short | Anatomomopathological and immunohistochemical analyses of the spleen and lymph node of dogs seropositives for leishmaniasis in serological tests |
| title_sort | anatomomopathological and immunohistochemical analyses of the spleen and lymph node of dogs seropositives for leishmaniasis in serological tests |
| url | https://revistas.ufg.br/vet/article/view/68909 |
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