Impacts of <i>Mir146a</i> Genotypes on Bladder Cancer Risk in Taiwan
The aim of this study was to investigate the association between single-nucleotide polymorphisms (SNPs) in <i>mir146a</i> and <i>mir196a</i> and bladder cancer (BLCA) risk in Taiwan. The genotypes of <i>mir146a</i> rs2910164 and <i>mir196a</i> rs116149...
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MDPI AG
2023-05-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/5/1396 |
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| author | Bo-Ren Wang Wen-Shin Chang Cheng-Hsi Liao Yun-Chi Wang Jian Gu Da-Tian Bau Chia-Wen Tsai |
| author_facet | Bo-Ren Wang Wen-Shin Chang Cheng-Hsi Liao Yun-Chi Wang Jian Gu Da-Tian Bau Chia-Wen Tsai |
| author_sort | Bo-Ren Wang |
| collection | DOAJ |
| description | The aim of this study was to investigate the association between single-nucleotide polymorphisms (SNPs) in <i>mir146a</i> and <i>mir196a</i> and bladder cancer (BLCA) risk in Taiwan. The genotypes of <i>mir146a</i> rs2910164 and <i>mir196a</i> rs11614913 were determined in 375 BLCA patients and 375 healthy controls using PCR-RFLP methodology, and their associations with BLCA risk were evaluated. The study also measured the serum expression level of <i>mir146a</i> using quantitative RT-PCR. The results showed that the distributions of CC, CG and GG genotypes of <i>mir146a</i> rs2910164 were 31.7%, 45.6% and 22.7% in the control group, and 21.9%, 44.3% and 33.8% in the case group, respectively. In logistic regression analyses, the heterozygous variant genotype CG carriers showed a marginally significant association with increased BLCA risk (OR = 1.41, 95% CI = 0.99–2.01), while the homozygous variant genotype GG carriers had a 2.17-fold increased risk of BLCA (OR = 2.17, 95%CI = 1.46–3.21). Moreover, carriers of the GG/CG genotypes had significantly higher serum levels of <i>mir146a</i> than those with the CC genotype (<i>p</i> < 0.0001), indicating a genotype–phenotype correlation. In contrast, <i>mir196a</i> rs11614913 was not associated with BLCA risk. Therefore, the genotypes of <i>mir146a</i> rs2910164 may serve as a useful biomarker for predicting the risk of BLCA. |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-11T03:55:18Z |
| publishDate | 2023-05-01 |
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| series | Biomedicines |
| spelling | doaj.art-fce3251837d448e49632cf5c3c2a60652023-11-18T00:36:31ZengMDPI AGBiomedicines2227-90592023-05-01115139610.3390/biomedicines11051396Impacts of <i>Mir146a</i> Genotypes on Bladder Cancer Risk in TaiwanBo-Ren Wang0Wen-Shin Chang1Cheng-Hsi Liao2Yun-Chi Wang3Jian Gu4Da-Tian Bau5Chia-Wen Tsai6Graduate Institute of Biomedical Sciences, China Medical University, Taichung 404333, TaiwanGraduate Institute of Biomedical Sciences, China Medical University, Taichung 404333, TaiwanGraduate Institute of Biomedical Sciences, China Medical University, Taichung 404333, TaiwanGraduate Institute of Biomedical Sciences, China Medical University, Taichung 404333, TaiwanDepartment of Epidemiology, The University of Texas MD Anderson Cancer Center, Houston, TX 77030, USAGraduate Institute of Biomedical Sciences, China Medical University, Taichung 404333, TaiwanGraduate Institute of Biomedical Sciences, China Medical University, Taichung 404333, TaiwanThe aim of this study was to investigate the association between single-nucleotide polymorphisms (SNPs) in <i>mir146a</i> and <i>mir196a</i> and bladder cancer (BLCA) risk in Taiwan. The genotypes of <i>mir146a</i> rs2910164 and <i>mir196a</i> rs11614913 were determined in 375 BLCA patients and 375 healthy controls using PCR-RFLP methodology, and their associations with BLCA risk were evaluated. The study also measured the serum expression level of <i>mir146a</i> using quantitative RT-PCR. The results showed that the distributions of CC, CG and GG genotypes of <i>mir146a</i> rs2910164 were 31.7%, 45.6% and 22.7% in the control group, and 21.9%, 44.3% and 33.8% in the case group, respectively. In logistic regression analyses, the heterozygous variant genotype CG carriers showed a marginally significant association with increased BLCA risk (OR = 1.41, 95% CI = 0.99–2.01), while the homozygous variant genotype GG carriers had a 2.17-fold increased risk of BLCA (OR = 2.17, 95%CI = 1.46–3.21). Moreover, carriers of the GG/CG genotypes had significantly higher serum levels of <i>mir146a</i> than those with the CC genotype (<i>p</i> < 0.0001), indicating a genotype–phenotype correlation. In contrast, <i>mir196a</i> rs11614913 was not associated with BLCA risk. Therefore, the genotypes of <i>mir146a</i> rs2910164 may serve as a useful biomarker for predicting the risk of BLCA.https://www.mdpi.com/2227-9059/11/5/1396bladder cancergenotype<i>mir146a</i><i>mir196a</i>phenotypesingle-nucleotide polymorphism |
| spellingShingle | Bo-Ren Wang Wen-Shin Chang Cheng-Hsi Liao Yun-Chi Wang Jian Gu Da-Tian Bau Chia-Wen Tsai Impacts of <i>Mir146a</i> Genotypes on Bladder Cancer Risk in Taiwan Biomedicines bladder cancer genotype <i>mir146a</i> <i>mir196a</i> phenotype single-nucleotide polymorphism |
| title | Impacts of <i>Mir146a</i> Genotypes on Bladder Cancer Risk in Taiwan |
| title_full | Impacts of <i>Mir146a</i> Genotypes on Bladder Cancer Risk in Taiwan |
| title_fullStr | Impacts of <i>Mir146a</i> Genotypes on Bladder Cancer Risk in Taiwan |
| title_full_unstemmed | Impacts of <i>Mir146a</i> Genotypes on Bladder Cancer Risk in Taiwan |
| title_short | Impacts of <i>Mir146a</i> Genotypes on Bladder Cancer Risk in Taiwan |
| title_sort | impacts of i mir146a i genotypes on bladder cancer risk in taiwan |
| topic | bladder cancer genotype <i>mir146a</i> <i>mir196a</i> phenotype single-nucleotide polymorphism |
| url | https://www.mdpi.com/2227-9059/11/5/1396 |
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