From Stable to Lab—Investigating Key Factors for Sudden Deaths Caused by <i>Streptococcus suis</i>
Swine stocks are endemically infected with the major porcine pathogen <i>Streptococcus </i>(<i>S.</i>)<i> suis</i>. The factors governing the transition from colonizing <i>S. suis</i> residing in the tonsils and the exacerbation of disease have not yet...
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| Format: | Article |
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MDPI AG
2019-11-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/8/4/249 |
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| author | Isabel Hennig-Pauka Rabea Imker Leonie Mayer Michael Brügmann Christiane Werckenthin Heike Weber Andrea Menrath Nicole de Buhr |
| author_facet | Isabel Hennig-Pauka Rabea Imker Leonie Mayer Michael Brügmann Christiane Werckenthin Heike Weber Andrea Menrath Nicole de Buhr |
| author_sort | Isabel Hennig-Pauka |
| collection | DOAJ |
| description | Swine stocks are endemically infected with the major porcine pathogen <i>Streptococcus </i>(<i>S.</i>)<i> suis</i>. The factors governing the transition from colonizing <i>S. suis</i> residing in the tonsils and the exacerbation of disease have not yet been elucidated. We analyzed the sudden death of fattening pigs kept under extensive husbandry conditions in a zoo. The animals died suddenly of septic shock and showed disseminated intravascular coagulopathy. Genotypic and phenotypic characterizations of the isolated <i>S. suis </i>strains, a tonsillar isolate and an invasive <i>cps</i> type 2 strain, were conducted. Isolated <i>S. suis </i>from dead pigs belonged to <i>cps</i> type 2 strain ST28, whereas one tonsillar <i>S. suis</i> isolate harvested from a healthy animal belonged to ST1173. Neither <i>S. suis</i> growth, induction of neutrophil extracellular traps, nor survival in blood could explain the sudden deaths. Reconstituted blood assays with serum samples from pigs of different age groups from the zoo stock suggested varying protection of individuals against pathogenic <i>cps</i> type 2 strains especially in younger pigs. These findings highlight the benefit of further characterization of the causative strains in each case by sequence typing before autologous vaccine candidate selection. |
| first_indexed | 2024-04-13T07:07:17Z |
| format | Article |
| id | doaj.art-fce4861bf2f74a32bf86f2c6b841b01b |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-04-13T07:07:17Z |
| publishDate | 2019-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-fce4861bf2f74a32bf86f2c6b841b01b2022-12-22T02:56:58ZengMDPI AGPathogens2076-08172019-11-018424910.3390/pathogens8040249pathogens8040249From Stable to Lab—Investigating Key Factors for Sudden Deaths Caused by <i>Streptococcus suis</i>Isabel Hennig-Pauka0Rabea Imker1Leonie Mayer2Michael Brügmann3Christiane Werckenthin4Heike Weber5Andrea Menrath6Nicole de Buhr7Field Station for Epidemiology, University of Veterinary Medicine Hannover, 30559 Hannover, GermanyDepartment of Physiological Chemistry, University of Veterinary Medicine Hannover, 30559 Hannover, GermanyInstitute of Bacteriology and Mycology, Centre for Infectious Diseases, Veterinary Faculty, University of Leipzig, 04109 Leipzig, GermanyFood and Veterinary Institute Oldenburg, Lower Saxony State Office for Consumer Protection and Food Safety, 26029 Oldenburg, GermanyFood and Veterinary Institute Oldenburg, Lower Saxony State Office for Consumer Protection and Food Safety, 26029 Oldenburg, GermanyTierpark Nordhorn, 48531 Nordhorn, GermanyField Station for Epidemiology, University of Veterinary Medicine Hannover, 30559 Hannover, GermanyDepartment of Physiological Chemistry, University of Veterinary Medicine Hannover, 30559 Hannover, GermanySwine stocks are endemically infected with the major porcine pathogen <i>Streptococcus </i>(<i>S.</i>)<i> suis</i>. The factors governing the transition from colonizing <i>S. suis</i> residing in the tonsils and the exacerbation of disease have not yet been elucidated. We analyzed the sudden death of fattening pigs kept under extensive husbandry conditions in a zoo. The animals died suddenly of septic shock and showed disseminated intravascular coagulopathy. Genotypic and phenotypic characterizations of the isolated <i>S. suis </i>strains, a tonsillar isolate and an invasive <i>cps</i> type 2 strain, were conducted. Isolated <i>S. suis </i>from dead pigs belonged to <i>cps</i> type 2 strain ST28, whereas one tonsillar <i>S. suis</i> isolate harvested from a healthy animal belonged to ST1173. Neither <i>S. suis</i> growth, induction of neutrophil extracellular traps, nor survival in blood could explain the sudden deaths. Reconstituted blood assays with serum samples from pigs of different age groups from the zoo stock suggested varying protection of individuals against pathogenic <i>cps</i> type 2 strains especially in younger pigs. These findings highlight the benefit of further characterization of the causative strains in each case by sequence typing before autologous vaccine candidate selection.https://www.mdpi.com/2076-0817/8/4/249streptococcus suissudden deathdicnetsimmune reaction |
| spellingShingle | Isabel Hennig-Pauka Rabea Imker Leonie Mayer Michael Brügmann Christiane Werckenthin Heike Weber Andrea Menrath Nicole de Buhr From Stable to Lab—Investigating Key Factors for Sudden Deaths Caused by <i>Streptococcus suis</i> Pathogens streptococcus suis sudden death dic nets immune reaction |
| title | From Stable to Lab—Investigating Key Factors for Sudden Deaths Caused by <i>Streptococcus suis</i> |
| title_full | From Stable to Lab—Investigating Key Factors for Sudden Deaths Caused by <i>Streptococcus suis</i> |
| title_fullStr | From Stable to Lab—Investigating Key Factors for Sudden Deaths Caused by <i>Streptococcus suis</i> |
| title_full_unstemmed | From Stable to Lab—Investigating Key Factors for Sudden Deaths Caused by <i>Streptococcus suis</i> |
| title_short | From Stable to Lab—Investigating Key Factors for Sudden Deaths Caused by <i>Streptococcus suis</i> |
| title_sort | from stable to lab investigating key factors for sudden deaths caused by i streptococcus suis i |
| topic | streptococcus suis sudden death dic nets immune reaction |
| url | https://www.mdpi.com/2076-0817/8/4/249 |
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