Fabrication of DNA micropatterns on the polycarbonate surface of compact discs
<p>Abstract</p><p>DNA micropatterns have been fabricated on the surface of Polycarbonate (PC) bases of compact discs, with the ultimate goal of using conventional CD technology for DNA analysis. To confirm the formation of -COOH groups on the PC surface upon UV/ozone treatment, con...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2006-01-01
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| Series: | Nanoscale Research Letters |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1007/s11671-006-9032-6 |
| _version_ | 1827883880242216960 |
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| author | Wang Zhen Li Rong-Xian |
| author_facet | Wang Zhen Li Rong-Xian |
| author_sort | Wang Zhen |
| collection | DOAJ |
| description | <p>Abstract</p><p>DNA micropatterns have been fabricated on the surface of Polycarbonate (PC) bases of compact discs, with the ultimate goal of using conventional CD technology for DNA analysis. To confirm the formation of -COOH groups on the PC surface upon UV/ozone treatment, contact angle titrations were carried out on the PC surfaces. The surface morphologies of PC surface were measured by Tapping-mode Atomic Force Microscopy (AFM). As the surface as “anchor” to attach the amine-modified ssDNA by covalent conjugates via amide bonds, the results of fluorescence spectroscopy showed that the fluorescein labeled complementary ssDNA can be used for detection the ssDNA immobilized on the patterned CD polycarbonate substrate.</p> |
| first_indexed | 2024-03-12T19:18:30Z |
| format | Article |
| id | doaj.art-fce618fe0cfe49208bdb79ec99902b95 |
| institution | Directory Open Access Journal |
| issn | 1931-7573 1556-276X |
| language | English |
| last_indexed | 2024-03-12T19:18:30Z |
| publishDate | 2006-01-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Nanoscale Research Letters |
| spelling | doaj.art-fce618fe0cfe49208bdb79ec99902b952023-08-02T05:22:27ZengSpringerOpenNanoscale Research Letters1931-75731556-276X2006-01-01226974Fabrication of DNA micropatterns on the polycarbonate surface of compact discsWang ZhenLi Rong-Xian<p>Abstract</p><p>DNA micropatterns have been fabricated on the surface of Polycarbonate (PC) bases of compact discs, with the ultimate goal of using conventional CD technology for DNA analysis. To confirm the formation of -COOH groups on the PC surface upon UV/ozone treatment, contact angle titrations were carried out on the PC surfaces. The surface morphologies of PC surface were measured by Tapping-mode Atomic Force Microscopy (AFM). As the surface as “anchor” to attach the amine-modified ssDNA by covalent conjugates via amide bonds, the results of fluorescence spectroscopy showed that the fluorescein labeled complementary ssDNA can be used for detection the ssDNA immobilized on the patterned CD polycarbonate substrate.</p>http://dx.doi.org/10.1007/s11671-006-9032-6DNACompact discUV/ozoneHybridization |
| spellingShingle | Wang Zhen Li Rong-Xian Fabrication of DNA micropatterns on the polycarbonate surface of compact discs Nanoscale Research Letters DNA Compact disc UV/ozone Hybridization |
| title | Fabrication of DNA micropatterns on the polycarbonate surface of compact discs |
| title_full | Fabrication of DNA micropatterns on the polycarbonate surface of compact discs |
| title_fullStr | Fabrication of DNA micropatterns on the polycarbonate surface of compact discs |
| title_full_unstemmed | Fabrication of DNA micropatterns on the polycarbonate surface of compact discs |
| title_short | Fabrication of DNA micropatterns on the polycarbonate surface of compact discs |
| title_sort | fabrication of dna micropatterns on the polycarbonate surface of compact discs |
| topic | DNA Compact disc UV/ozone Hybridization |
| url | http://dx.doi.org/10.1007/s11671-006-9032-6 |
| work_keys_str_mv | AT wangzhen fabricationofdnamicropatternsonthepolycarbonatesurfaceofcompactdiscs AT lirongxian fabricationofdnamicropatternsonthepolycarbonatesurfaceofcompactdiscs |