Mupirocin Promotes Wound Healing by Stimulating Growth Factor Production and Proliferation of Human Keratinocytes
Mupirocin has been reported for its role in the treatment of infected wounds through its antibacterial activity, however the role of mupirocin in promoting wound healing via alternative mechanisms has not been extensively evaluated. This study aimed to evaluate the potential effect of mupirocin to p...
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2022.862112/full |
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| author | Danielle Twilley Oleg Reva Debra Meyer Namrita Lall Namrita Lall Namrita Lall Namrita Lall |
| author_facet | Danielle Twilley Oleg Reva Debra Meyer Namrita Lall Namrita Lall Namrita Lall Namrita Lall |
| author_sort | Danielle Twilley |
| collection | DOAJ |
| description | Mupirocin has been reported for its role in the treatment of infected wounds through its antibacterial activity, however the role of mupirocin in promoting wound healing via alternative mechanisms has not been extensively evaluated. This study aimed to evaluate the potential effect of mupirocin to promote wound healing, not only through its antibacterial activity but by increasing human keratinocyte proliferation and growth factor production. In the scratch assay, using human keratinocytes (HaCat), mupirocin (at 0.1 and 0.2 mM) significantly increased wound closure compared to the vehicle control. Cell viability, measured from the scratch assay, verified the increase in wound closure, where mupirocin at both concentrations showed higher cell viability compared to the vehicle control. In addition, mupirocin at 0.1 mM significantly stimulated the production of hepatocyte growth factor and M-CSF in HaCat cells, whereas at 0.2 mM, PDGF-AA and EPO were increased. The findings of this study suggest that mupirocin, which is commonly used as an antibacterial agent for the treatment of wounds, also facilitates the wound healing process by stimulating the proliferation of human keratinocytes and enhancing the production of several growth factors involved in wound healing. This is the first report on the effect of mupirocin on growth factors expressed by human keratinocytes as well as the stimulation of keratinocyte proliferation. |
| first_indexed | 2024-04-12T22:25:43Z |
| format | Article |
| id | doaj.art-fce6690096b94127b5139f48c66ede07 |
| institution | Directory Open Access Journal |
| issn | 1663-9812 |
| language | English |
| last_indexed | 2024-04-12T22:25:43Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj.art-fce6690096b94127b5139f48c66ede072022-12-22T03:14:09ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122022-04-011310.3389/fphar.2022.862112862112Mupirocin Promotes Wound Healing by Stimulating Growth Factor Production and Proliferation of Human KeratinocytesDanielle Twilley0Oleg Reva1Debra Meyer2Namrita Lall3Namrita Lall4Namrita Lall5Namrita Lall6Department of Plant and Soil Sciences, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria, South AfricaDepartment of Biochemistry, Genetics and Microbiology, Faculty of Natural and Agricultural Sciences, Centre for Bioinformatics and Computational Biology, University of Pretoria, Pretoria, South AfricaDepartment of Biochemistry, Faculty of Science, University of Johannesburg, Johannesburg, South AfricaDepartment of Plant and Soil Sciences, Faculty of Natural and Agricultural Sciences, University of Pretoria, Pretoria, South AfricaSchool of Natural Resources, College of Agriculture, Food and Natural Resources, University of Missouri, Columbia, MO, United StatesCollege of Pharmacy, JSS Academy of Higher Education and Research, Mysuru, IndiaBio-Tech Research and Development Institute, University of the West Indies, Kingston, JamaicaMupirocin has been reported for its role in the treatment of infected wounds through its antibacterial activity, however the role of mupirocin in promoting wound healing via alternative mechanisms has not been extensively evaluated. This study aimed to evaluate the potential effect of mupirocin to promote wound healing, not only through its antibacterial activity but by increasing human keratinocyte proliferation and growth factor production. In the scratch assay, using human keratinocytes (HaCat), mupirocin (at 0.1 and 0.2 mM) significantly increased wound closure compared to the vehicle control. Cell viability, measured from the scratch assay, verified the increase in wound closure, where mupirocin at both concentrations showed higher cell viability compared to the vehicle control. In addition, mupirocin at 0.1 mM significantly stimulated the production of hepatocyte growth factor and M-CSF in HaCat cells, whereas at 0.2 mM, PDGF-AA and EPO were increased. The findings of this study suggest that mupirocin, which is commonly used as an antibacterial agent for the treatment of wounds, also facilitates the wound healing process by stimulating the proliferation of human keratinocytes and enhancing the production of several growth factors involved in wound healing. This is the first report on the effect of mupirocin on growth factors expressed by human keratinocytes as well as the stimulation of keratinocyte proliferation.https://www.frontiersin.org/articles/10.3389/fphar.2022.862112/fullmupirocinhuman keratinocyteswound healingcell proliferationhuman growth factors |
| spellingShingle | Danielle Twilley Oleg Reva Debra Meyer Namrita Lall Namrita Lall Namrita Lall Namrita Lall Mupirocin Promotes Wound Healing by Stimulating Growth Factor Production and Proliferation of Human Keratinocytes Frontiers in Pharmacology mupirocin human keratinocytes wound healing cell proliferation human growth factors |
| title | Mupirocin Promotes Wound Healing by Stimulating Growth Factor Production and Proliferation of Human Keratinocytes |
| title_full | Mupirocin Promotes Wound Healing by Stimulating Growth Factor Production and Proliferation of Human Keratinocytes |
| title_fullStr | Mupirocin Promotes Wound Healing by Stimulating Growth Factor Production and Proliferation of Human Keratinocytes |
| title_full_unstemmed | Mupirocin Promotes Wound Healing by Stimulating Growth Factor Production and Proliferation of Human Keratinocytes |
| title_short | Mupirocin Promotes Wound Healing by Stimulating Growth Factor Production and Proliferation of Human Keratinocytes |
| title_sort | mupirocin promotes wound healing by stimulating growth factor production and proliferation of human keratinocytes |
| topic | mupirocin human keratinocytes wound healing cell proliferation human growth factors |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2022.862112/full |
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