Characterization of mother palms and novel techniques to produce elite seedlings of coconut var. Chowghat Orange Dwarf
Coconut is one of the major plantation crops of India and the demand for elite seedlings is exorbitant. In this study, superior mother palms of Chowghat Orange Dwarf (COD) which is not completely homozygous were identified based on Phenotypic Co-efficient of Variation (PCV), Genotypic Co-efficie...
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| Format: | Article |
| Language: | English |
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Indian Society of Plant Breeders
2023-09-01
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| Series: | Electronic Journal of Plant Breeding |
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| Online Access: | https://ejplantbreeding.org/index.php/EJPB/article/view/4295 |
| _version_ | 1797662096495738880 |
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| author | S. Rohith1 , S. Kavibalan1 , K. Thangaraj2 , J. Suresh4 , M. Ananthan3 and R. Renuka1* |
| author_facet | S. Rohith1 , S. Kavibalan1 , K. Thangaraj2 , J. Suresh4 , M. Ananthan3 and R. Renuka1* |
| author_sort | S. Rohith1 , S. Kavibalan1 , K. Thangaraj2 , J. Suresh4 , M. Ananthan3 and R. Renuka1* |
| collection | DOAJ |
| description | Coconut is one of the major plantation crops of India and the demand for elite seedlings is exorbitant. In this study,
superior mother palms of Chowghat Orange Dwarf (COD) which is not completely homozygous were identified based
on Phenotypic Co-efficient of Variation (PCV), Genotypic Co-efficient of Variation (GCV), heritability and genetic
advance of morphological traits for applications in crop improvement. Explants from superior palms viz., mature
embryos (whole/sliced) and shoot tips (whole/sliced), were used for direct organogenesis. Among these, sliced shoot
tips (4 slices) in Y3 basal + 200 µM Thidiazuran (TDZ), recorded highest shoot induction (350 %) and regeneration
frequency (325%) at 30 Days After Inoculation (DAI) and 60 DAI respectively than sliced embryos (4 slices) which
recorded lesser shoot induction (170 %) and regeneration frequency (125%). This is the first study that demonstrates
shoot tips as one of the potential explants for in-vitro production of quality coconut seedlings. |
| first_indexed | 2024-03-11T18:55:09Z |
| format | Article |
| id | doaj.art-fce7dfeb90574159b999cd5d614b40f9 |
| institution | Directory Open Access Journal |
| issn | 0975-928X |
| language | English |
| last_indexed | 2024-03-11T18:55:09Z |
| publishDate | 2023-09-01 |
| publisher | Indian Society of Plant Breeders |
| record_format | Article |
| series | Electronic Journal of Plant Breeding |
| spelling | doaj.art-fce7dfeb90574159b999cd5d614b40f92023-10-11T06:47:41ZengIndian Society of Plant BreedersElectronic Journal of Plant Breeding0975-928X2023-09-01143867 875https://doi.org/10.37992/2023.1403.112Characterization of mother palms and novel techniques to produce elite seedlings of coconut var. Chowghat Orange DwarfS. Rohith1 , S. Kavibalan1 , K. Thangaraj2 , J. Suresh4 , M. Ananthan3 and R. Renuka1*01 Department of Plant Biotechnology, Centre for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore -641 003 2 Centre of Innovation, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai – 625 104 3 Department of Horticulture, Agricultural College and Research Institute, Tamil Nadu Agricultural University, Madurai – 625 104 4 Coconut Research Station, Aliyar Nagar, Coimbatore – 642 101 *E-Mail: renukaraman@tnau.ac.inCoconut is one of the major plantation crops of India and the demand for elite seedlings is exorbitant. In this study, superior mother palms of Chowghat Orange Dwarf (COD) which is not completely homozygous were identified based on Phenotypic Co-efficient of Variation (PCV), Genotypic Co-efficient of Variation (GCV), heritability and genetic advance of morphological traits for applications in crop improvement. Explants from superior palms viz., mature embryos (whole/sliced) and shoot tips (whole/sliced), were used for direct organogenesis. Among these, sliced shoot tips (4 slices) in Y3 basal + 200 µM Thidiazuran (TDZ), recorded highest shoot induction (350 %) and regeneration frequency (325%) at 30 Days After Inoculation (DAI) and 60 DAI respectively than sliced embryos (4 slices) which recorded lesser shoot induction (170 %) and regeneration frequency (125%). This is the first study that demonstrates shoot tips as one of the potential explants for in-vitro production of quality coconut seedlings.https://ejplantbreeding.org/index.php/EJPB/article/view/4295coconutgenetic variabilitydirect organogenesismature embryo culture and shoot tip culture |
| spellingShingle | S. Rohith1 , S. Kavibalan1 , K. Thangaraj2 , J. Suresh4 , M. Ananthan3 and R. Renuka1* Characterization of mother palms and novel techniques to produce elite seedlings of coconut var. Chowghat Orange Dwarf Electronic Journal of Plant Breeding coconut genetic variability direct organogenesis mature embryo culture and shoot tip culture |
| title | Characterization of mother palms and novel techniques to produce elite seedlings of coconut var. Chowghat Orange Dwarf |
| title_full | Characterization of mother palms and novel techniques to produce elite seedlings of coconut var. Chowghat Orange Dwarf |
| title_fullStr | Characterization of mother palms and novel techniques to produce elite seedlings of coconut var. Chowghat Orange Dwarf |
| title_full_unstemmed | Characterization of mother palms and novel techniques to produce elite seedlings of coconut var. Chowghat Orange Dwarf |
| title_short | Characterization of mother palms and novel techniques to produce elite seedlings of coconut var. Chowghat Orange Dwarf |
| title_sort | characterization of mother palms and novel techniques to produce elite seedlings of coconut var chowghat orange dwarf |
| topic | coconut genetic variability direct organogenesis mature embryo culture and shoot tip culture |
| url | https://ejplantbreeding.org/index.php/EJPB/article/view/4295 |
| work_keys_str_mv | AT srohith1skavibalan1kthangaraj2jsuresh4mananthan3andrrenuka1 characterizationofmotherpalmsandnoveltechniquestoproduceeliteseedlingsofcoconutvarchowghatorangedwarf |