Oxidative stress predominates apoptosis during experimental hepatocellular carcinoma
Objective Hepatocellular carcinoma ranks the third among cancer deaths in the globe. Despite the fact that many strategies for diagnosing liver cancer are possible, many factors, including apoptosis, arise to interfere with cell cycle control. We initiated this study to investigate the importance of...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nab'a Al-Hayat Foundation for Medical Sciences and Health Care - Press
2017-11-01
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| Series: | Journal of Contemporary Medical Sciences |
| Online Access: | http://www.jocms.org/index.php/jcms/article/view/267 |
| _version_ | 1818570868612136960 |
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| author | Nabil M. Abdel-Hamid Afrah F. Salama Mostafa El-Sheekh Naglaa Sarhan Asmaa M. Gabr |
| author_facet | Nabil M. Abdel-Hamid Afrah F. Salama Mostafa El-Sheekh Naglaa Sarhan Asmaa M. Gabr |
| author_sort | Nabil M. Abdel-Hamid |
| collection | DOAJ |
| description | Objective Hepatocellular carcinoma ranks the third among cancer deaths in the globe. Despite the fact that many strategies for diagnosing liver cancer are possible, many factors, including apoptosis, arise to interfere with cell cycle control. We initiated this study to investigate the importance of free radicals during experimental hepatocarcinogenesis, triggered by diethyl nitrosamine, against an anti-apoptotic factor, belongs to a family of oncogenes (Bcl-2).
Methods Eighteen female Wistar rats were classified into two equal groups, group 1 received intraperitoneal normal saline doses, group 2 was injected with one IP dose of diethyl nitrosamine (200 mg/kg bw), 2 weeks later, they were given a single CCl4 dose (2 ml /kg b.w., IP), blood and liver tissue samples were collected after 60 days of diethyl nitrosamine dose. The Graph pad prism program was used in statistical calculations.
Results The results revealed that treatment with diethyl nitrosamine + CCl4 significantly up regulated the plasma liver functions tests: aspartate transaminase, alanine transaminase, gamma-glutamyl transpeptidase, total bilirubin and increased alpha fetoprotein with decreased level of Bcl-2. Liver tissue showed elevation of lipid peroxide, malondialdehyde, catalase enzyme activity, with significant reduction in body weight, decreased total protein content, tissue antioxidants like glutathione S-transferase, total thiol and total antioxidant capacity, compared to control. Histological examination of liver showed an increased nuclear/cytoplasm (N/C) ratio, mitotic figures and nuclear polymorphism and microacinar formation in diethyl nitrosamine + CCl4-treated group.
Conclusions Disturbed hepatocellular anti-oxidant mechanisms suppress apoptosis, reflecting failure in combating chemical
hepatocarcinogenesis.
Keywords liver cancer; diethyl nitrosamine; lipid peroxidation; tumor markers; Bcl-2; apoptosis. |
| first_indexed | 2024-12-14T13:47:36Z |
| format | Article |
| id | doaj.art-fce8d503c051409bae25f84bd50cab33 |
| institution | Directory Open Access Journal |
| issn | 2415-1629 2413-0516 |
| language | English |
| last_indexed | 2024-12-14T13:47:36Z |
| publishDate | 2017-11-01 |
| publisher | Nab'a Al-Hayat Foundation for Medical Sciences and Health Care - Press |
| record_format | Article |
| series | Journal of Contemporary Medical Sciences |
| spelling | doaj.art-fce8d503c051409bae25f84bd50cab332022-12-21T22:59:16ZengNab'a Al-Hayat Foundation for Medical Sciences and Health Care - PressJournal of Contemporary Medical Sciences2415-16292413-05162017-11-01312295299267Oxidative stress predominates apoptosis during experimental hepatocellular carcinomaNabil M. Abdel-Hamid0Afrah F. Salama1Mostafa El-Sheekh2Naglaa Sarhan3Asmaa M. Gabr4Biochemistry Department, Faculty of Pharmacy, Kafrelsheikh University, Egypt.Chemistry Department, Biochemistry Section, Faculty of Science, Tanta University, Egypt.Botany Department, Faculty of Science, Tanta University, Egypt.Histopathology Department, Faculty of Medicine, Tanta University, Egypt.Chemistry Department, Biochemistry Section, Faculty of Science, Tanta University, Egypt.Objective Hepatocellular carcinoma ranks the third among cancer deaths in the globe. Despite the fact that many strategies for diagnosing liver cancer are possible, many factors, including apoptosis, arise to interfere with cell cycle control. We initiated this study to investigate the importance of free radicals during experimental hepatocarcinogenesis, triggered by diethyl nitrosamine, against an anti-apoptotic factor, belongs to a family of oncogenes (Bcl-2). Methods Eighteen female Wistar rats were classified into two equal groups, group 1 received intraperitoneal normal saline doses, group 2 was injected with one IP dose of diethyl nitrosamine (200 mg/kg bw), 2 weeks later, they were given a single CCl4 dose (2 ml /kg b.w., IP), blood and liver tissue samples were collected after 60 days of diethyl nitrosamine dose. The Graph pad prism program was used in statistical calculations. Results The results revealed that treatment with diethyl nitrosamine + CCl4 significantly up regulated the plasma liver functions tests: aspartate transaminase, alanine transaminase, gamma-glutamyl transpeptidase, total bilirubin and increased alpha fetoprotein with decreased level of Bcl-2. Liver tissue showed elevation of lipid peroxide, malondialdehyde, catalase enzyme activity, with significant reduction in body weight, decreased total protein content, tissue antioxidants like glutathione S-transferase, total thiol and total antioxidant capacity, compared to control. Histological examination of liver showed an increased nuclear/cytoplasm (N/C) ratio, mitotic figures and nuclear polymorphism and microacinar formation in diethyl nitrosamine + CCl4-treated group. Conclusions Disturbed hepatocellular anti-oxidant mechanisms suppress apoptosis, reflecting failure in combating chemical hepatocarcinogenesis. Keywords liver cancer; diethyl nitrosamine; lipid peroxidation; tumor markers; Bcl-2; apoptosis.http://www.jocms.org/index.php/jcms/article/view/267 |
| spellingShingle | Nabil M. Abdel-Hamid Afrah F. Salama Mostafa El-Sheekh Naglaa Sarhan Asmaa M. Gabr Oxidative stress predominates apoptosis during experimental hepatocellular carcinoma Journal of Contemporary Medical Sciences |
| title | Oxidative stress predominates apoptosis during experimental hepatocellular carcinoma |
| title_full | Oxidative stress predominates apoptosis during experimental hepatocellular carcinoma |
| title_fullStr | Oxidative stress predominates apoptosis during experimental hepatocellular carcinoma |
| title_full_unstemmed | Oxidative stress predominates apoptosis during experimental hepatocellular carcinoma |
| title_short | Oxidative stress predominates apoptosis during experimental hepatocellular carcinoma |
| title_sort | oxidative stress predominates apoptosis during experimental hepatocellular carcinoma |
| url | http://www.jocms.org/index.php/jcms/article/view/267 |
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