Cytotoxic Activity of 2-O-β-glucopyranosil Cucurbitacin D from Benalu Batu (<i>Begonia</i> sp.) Growing in Morowali, Central Sulawesi
Benalu batu (Begonia sp.) had been used traditionally as an anticancer medicinal plant by Wana tribe in Morowali, Central Sulawesi, This study aims to evaluate the cytotoxic activity of 2-O-β-glucopyranosil cucurbitacin D, isolated from the ethyl acetate soluble fraction of Benalu batu (Begonia sp.)...
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| Format: | Article |
| Language: | English |
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Department of Chemistry, Universitas Gadjah Mada
2020-06-01
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| Series: | Indonesian Journal of Chemistry |
| Subjects: | |
| Online Access: | https://jurnal.ugm.ac.id/ijc/article/view/43626 |
| _version_ | 1819009207294230528 |
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| author | Muhammad Sulaiman Zubair Walied Mohamed Alarif Mohamed Ali Ghandourah Syariful Anam Ibrahim Jantan |
| author_facet | Muhammad Sulaiman Zubair Walied Mohamed Alarif Mohamed Ali Ghandourah Syariful Anam Ibrahim Jantan |
| author_sort | Muhammad Sulaiman Zubair |
| collection | DOAJ |
| description | Benalu batu (Begonia sp.) had been used traditionally as an anticancer medicinal plant by Wana tribe in Morowali, Central Sulawesi, This study aims to evaluate the cytotoxic activity of 2-O-β-glucopyranosil cucurbitacin D, isolated from the ethyl acetate soluble fraction of Benalu batu (Begonia sp.) and to determine its action on apoptosis induction. Benalu batu (Begonia sp.) herb was extracted by maceration using ethanol 96% as a solvent. Vacuum liquid column chromatography and preparative thin layer chromatography have been applied on fractionation and isolation of the compound. The structure elucidation was performed by extensive analysis of 1D/2D nuclear magnetic resonance (NMR) and Mass Spectrophotometer (MS). Cytotoxic activity against human breast adenocarcinoma (MCF-7) and human colon colorectal carcinoma (HCT-116) cell lines were performed by 5-diphenyltetrazolium bromide (MTT) method. Annexin V-FITC assay was employed to determine the apoptosis induction. 2-O-β-glucopyranosil cucurbitacin D showed potent cytotoxic activity against MCF-7 and HCT-116 with the IC50 of 19.913 and 0.002 μg/mL, respectively. Annexin V-FITC assay clearly exhibited the cytotoxic mechanism on MCF-7 and HCT-116 via apoptosis induction with a significant percentage of early and late apoptosis of 75.8 and 78.4%, respectively. This study reveals the potential cytotoxic activity of 2-O-β-glucopyranosil cucurbitacin D isolated from Benalu batu and its mechanism via apoptosis induction. |
| first_indexed | 2024-12-21T00:52:42Z |
| format | Article |
| id | doaj.art-fcea3cd5a2b549e9b38f6f420051cc80 |
| institution | Directory Open Access Journal |
| issn | 1411-9420 2460-1578 |
| language | English |
| last_indexed | 2024-12-21T00:52:42Z |
| publishDate | 2020-06-01 |
| publisher | Department of Chemistry, Universitas Gadjah Mada |
| record_format | Article |
| series | Indonesian Journal of Chemistry |
| spelling | doaj.art-fcea3cd5a2b549e9b38f6f420051cc802022-12-21T19:21:22ZengDepartment of Chemistry, Universitas Gadjah MadaIndonesian Journal of Chemistry1411-94202460-15782020-06-0120476677210.22146/ijc.4362625457Cytotoxic Activity of 2-O-β-glucopyranosil Cucurbitacin D from Benalu Batu (<i>Begonia</i> sp.) Growing in Morowali, Central SulawesiMuhammad Sulaiman Zubair0Walied Mohamed Alarif1Mohamed Ali Ghandourah2Syariful Anam3Ibrahim Jantan4Department of Pharmacy, Science Faculty, Tadulako University, Jl. Soekarno Hatta Tondo, Palu 94118, IndonesiaDepartment of Marine Chemistry, Marine Science Faculty, King Abdul Aziz University, Jeddah 21589, Saudi ArabiaDepartment of Marine Chemistry, Marine Science Faculty, King Abdul Aziz University, Jeddah 21589, Saudi ArabiaDepartment of Pharmacy, Science Faculty, Tadulako University, Jl. Soekarno Hatta Tondo, Palu 94118, IndonesiaSchool of Pharmacy, Taylor’s University, Lakeside Campus, Jl. Taylors No. 1, Subang Jaya 47500, Selangor, MalaysiaBenalu batu (Begonia sp.) had been used traditionally as an anticancer medicinal plant by Wana tribe in Morowali, Central Sulawesi, This study aims to evaluate the cytotoxic activity of 2-O-β-glucopyranosil cucurbitacin D, isolated from the ethyl acetate soluble fraction of Benalu batu (Begonia sp.) and to determine its action on apoptosis induction. Benalu batu (Begonia sp.) herb was extracted by maceration using ethanol 96% as a solvent. Vacuum liquid column chromatography and preparative thin layer chromatography have been applied on fractionation and isolation of the compound. The structure elucidation was performed by extensive analysis of 1D/2D nuclear magnetic resonance (NMR) and Mass Spectrophotometer (MS). Cytotoxic activity against human breast adenocarcinoma (MCF-7) and human colon colorectal carcinoma (HCT-116) cell lines were performed by 5-diphenyltetrazolium bromide (MTT) method. Annexin V-FITC assay was employed to determine the apoptosis induction. 2-O-β-glucopyranosil cucurbitacin D showed potent cytotoxic activity against MCF-7 and HCT-116 with the IC50 of 19.913 and 0.002 μg/mL, respectively. Annexin V-FITC assay clearly exhibited the cytotoxic mechanism on MCF-7 and HCT-116 via apoptosis induction with a significant percentage of early and late apoptosis of 75.8 and 78.4%, respectively. This study reveals the potential cytotoxic activity of 2-O-β-glucopyranosil cucurbitacin D isolated from Benalu batu and its mechanism via apoptosis induction.https://jurnal.ugm.ac.id/ijc/article/view/43626begonia sp.2-o-β-glucopyranosil cucurbitacin dcytotoxicmcf-7hct-116 |
| spellingShingle | Muhammad Sulaiman Zubair Walied Mohamed Alarif Mohamed Ali Ghandourah Syariful Anam Ibrahim Jantan Cytotoxic Activity of 2-O-β-glucopyranosil Cucurbitacin D from Benalu Batu (<i>Begonia</i> sp.) Growing in Morowali, Central Sulawesi Indonesian Journal of Chemistry begonia sp. 2-o-β-glucopyranosil cucurbitacin d cytotoxic mcf-7 hct-116 |
| title | Cytotoxic Activity of 2-O-β-glucopyranosil Cucurbitacin D from Benalu Batu (<i>Begonia</i> sp.) Growing in Morowali, Central Sulawesi |
| title_full | Cytotoxic Activity of 2-O-β-glucopyranosil Cucurbitacin D from Benalu Batu (<i>Begonia</i> sp.) Growing in Morowali, Central Sulawesi |
| title_fullStr | Cytotoxic Activity of 2-O-β-glucopyranosil Cucurbitacin D from Benalu Batu (<i>Begonia</i> sp.) Growing in Morowali, Central Sulawesi |
| title_full_unstemmed | Cytotoxic Activity of 2-O-β-glucopyranosil Cucurbitacin D from Benalu Batu (<i>Begonia</i> sp.) Growing in Morowali, Central Sulawesi |
| title_short | Cytotoxic Activity of 2-O-β-glucopyranosil Cucurbitacin D from Benalu Batu (<i>Begonia</i> sp.) Growing in Morowali, Central Sulawesi |
| title_sort | cytotoxic activity of 2 o β glucopyranosil cucurbitacin d from benalu batu i begonia i sp growing in morowali central sulawesi |
| topic | begonia sp. 2-o-β-glucopyranosil cucurbitacin d cytotoxic mcf-7 hct-116 |
| url | https://jurnal.ugm.ac.id/ijc/article/view/43626 |
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