Importance of macrophage cholesterol content on the flux of cholesterol mass
Net flux of cholesterol represents the difference between efflux and influx and can result in net cell-cholesterol accumulation, net cell-cholesterol depletion, or no change in cellular cholesterol content. We measured radiolabeled cell-cholesterol efflux and cell-cholesterol mass using cholesterol-...
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| Format: | Article |
| Language: | English |
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Elsevier
2010-11-01
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| Series: | Journal of Lipid Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520409575 |
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| author | Sandhya Sankaranarayanan Margarita de la Llera-Moya Denise Drazul-Schrader Bela F. Asztalos Ginny L. Weibel George H. Rothblat |
| author_facet | Sandhya Sankaranarayanan Margarita de la Llera-Moya Denise Drazul-Schrader Bela F. Asztalos Ginny L. Weibel George H. Rothblat |
| author_sort | Sandhya Sankaranarayanan |
| collection | DOAJ |
| description | Net flux of cholesterol represents the difference between efflux and influx and can result in net cell-cholesterol accumulation, net cell-cholesterol depletion, or no change in cellular cholesterol content. We measured radiolabeled cell-cholesterol efflux and cell-cholesterol mass using cholesterol-normal and -enriched J774 and elicited mouse peritoneal macrophage cells. Net cell-cholesterol effluxes were observed when cholesterol-enriched J774 cells were incubated with 3.5% apolipoprotein (apo) B depleted human serum, HDL3, and apo A-I. Net cell-cholesterol influxes were observed when cholesterol-normal J774 cells were incubated with the same acceptors except apo A-I. When incubated with 2.5% individual sera, cholesterol mass efflux in free cholesterol (FC)-enriched J774 cells correlated with the HDL-cholesterol (HDL-C) concentrations (r2= 0.4; P=0.003), whereas cholesterol mass influx in cholesterol-normal J774 cells correlated with the LDL cholesterol (LDL-C) concentrations (r2= 0.6; P<0.0001) of the individual sera. A positive correlation was observed between measurements of [3H]cholesterol efflux and reductions in cholesterol mass (r2= 0.4; P=0.001) in FC-enriched J774 cells. In conclusion, isotopic efflux measurements from cholesterol-normal or cholesterol-enriched cells provide an accurate measurement of relative ability of an acceptor to remove labeled cholesterol under a specific set of experimental conditions, i.e., efflux potential. Moreover, isotopic efflux measurements can reflect changes in cellular cholesterol mass if the donor cells are enriched with cholesterol. |
| first_indexed | 2024-12-18T01:40:48Z |
| format | Article |
| id | doaj.art-fcea3e9d3a4049389bd75ab70ea4cc38 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-18T01:40:48Z |
| publishDate | 2010-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-fcea3e9d3a4049389bd75ab70ea4cc382022-12-21T21:25:21ZengElsevierJournal of Lipid Research0022-22752010-11-01511132433249Importance of macrophage cholesterol content on the flux of cholesterol massSandhya Sankaranarayanan0Margarita de la Llera-Moya1Denise Drazul-Schrader2Bela F. Asztalos3Ginny L. Weibel4George H. Rothblat5Division of Gastroenterology, Hepatology, and Nutrition, The Children's Hospital of Philadelphia, Philadelphia, PADivision of Gastroenterology, Hepatology, and Nutrition, The Children's Hospital of Philadelphia, Philadelphia, PADivision of Gastroenterology, Hepatology, and Nutrition, The Children's Hospital of Philadelphia, Philadelphia, PALipid Metabolism Laboratory, Tufts University, Boston, MADivision of Gastroenterology, Hepatology, and Nutrition, The Children's Hospital of Philadelphia, Philadelphia, PATo whom correspondence should be addressed. rothblat@email.chop.edu; Division of Gastroenterology, Hepatology, and Nutrition, The Children's Hospital of Philadelphia, Philadelphia, PANet flux of cholesterol represents the difference between efflux and influx and can result in net cell-cholesterol accumulation, net cell-cholesterol depletion, or no change in cellular cholesterol content. We measured radiolabeled cell-cholesterol efflux and cell-cholesterol mass using cholesterol-normal and -enriched J774 and elicited mouse peritoneal macrophage cells. Net cell-cholesterol effluxes were observed when cholesterol-enriched J774 cells were incubated with 3.5% apolipoprotein (apo) B depleted human serum, HDL3, and apo A-I. Net cell-cholesterol influxes were observed when cholesterol-normal J774 cells were incubated with the same acceptors except apo A-I. When incubated with 2.5% individual sera, cholesterol mass efflux in free cholesterol (FC)-enriched J774 cells correlated with the HDL-cholesterol (HDL-C) concentrations (r2= 0.4; P=0.003), whereas cholesterol mass influx in cholesterol-normal J774 cells correlated with the LDL cholesterol (LDL-C) concentrations (r2= 0.6; P<0.0001) of the individual sera. A positive correlation was observed between measurements of [3H]cholesterol efflux and reductions in cholesterol mass (r2= 0.4; P=0.001) in FC-enriched J774 cells. In conclusion, isotopic efflux measurements from cholesterol-normal or cholesterol-enriched cells provide an accurate measurement of relative ability of an acceptor to remove labeled cholesterol under a specific set of experimental conditions, i.e., efflux potential. Moreover, isotopic efflux measurements can reflect changes in cellular cholesterol mass if the donor cells are enriched with cholesterol.http://www.sciencedirect.com/science/article/pii/S0022227520409575cholesterol-enrichedeffluxinfluxreverse cholesterol transport |
| spellingShingle | Sandhya Sankaranarayanan Margarita de la Llera-Moya Denise Drazul-Schrader Bela F. Asztalos Ginny L. Weibel George H. Rothblat Importance of macrophage cholesterol content on the flux of cholesterol mass Journal of Lipid Research cholesterol-enriched efflux influx reverse cholesterol transport |
| title | Importance of macrophage cholesterol content on the flux of cholesterol mass |
| title_full | Importance of macrophage cholesterol content on the flux of cholesterol mass |
| title_fullStr | Importance of macrophage cholesterol content on the flux of cholesterol mass |
| title_full_unstemmed | Importance of macrophage cholesterol content on the flux of cholesterol mass |
| title_short | Importance of macrophage cholesterol content on the flux of cholesterol mass |
| title_sort | importance of macrophage cholesterol content on the flux of cholesterol mass |
| topic | cholesterol-enriched efflux influx reverse cholesterol transport |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520409575 |
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