Anti-Inflammatory and Antipruritic Effects of Remote Ischaemic Postconditioning in a Mouse Model of Experimental Allergic Contact Dermatitis
<i>Background and Objectives:</i> Allergic contact dermatitis is a common type IV hypersensitivity reaction characterised by redness, itching, oedema and thickening of the skin. It occurs in about 7% of the population and its incidence is increasing. It has been observed that the precond...
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MDPI AG
2023-10-01
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| Series: | Medicina |
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| Online Access: | https://www.mdpi.com/1648-9144/59/10/1816 |
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| author | Ozgur Gunduz Melike Sapmaz-Metin Ruhan Deniz Topuz Oktay Kaya Cetin Hakan Karadag Ahmet Ulugol |
| author_facet | Ozgur Gunduz Melike Sapmaz-Metin Ruhan Deniz Topuz Oktay Kaya Cetin Hakan Karadag Ahmet Ulugol |
| author_sort | Ozgur Gunduz |
| collection | DOAJ |
| description | <i>Background and Objectives:</i> Allergic contact dermatitis is a common type IV hypersensitivity reaction characterised by redness, itching, oedema and thickening of the skin. It occurs in about 7% of the population and its incidence is increasing. It has been observed that the preconditioning of tissues by exposing them to transient ischemia increases resistance to subsequent permanent ischemia, and this phenomenon is called ischemic preconditioning. It has been shown that conditioning in one organ can also protect other organs. The protective effect of remote ischemic preconditioning is thought to be based on the induction of anti-inflammatory responses. The aim of this project was to investigate the anti-inflammatory and antipruritic effects of remote ischemic postconditioning in a mouse model of experimental allergic contact dermatitis. <i>Methods:</i> Experimental allergic contact dermatitis was induced with 1-fluoro-2,4-dinitrobenzene. Remote ischemic postconditioning was performed at 3 and 25 h after the challenge. Ear thickness and number of scratches 24 and 48 h after challenge, as well as cytokine levels and the infiltration of mast cells, neutrophils, CD4<sup>+</sup> and CD8<sup>+</sup> T lymphocytes in serum and ear tissue at 48 h were measured to determine the effect of RIPsC. <i>Results:</i> Remote ischemic postconditioning decreased ear thickness, one of the symptoms of allergic contact dermatitis (<i>p</i> < 0.0001). It had no significant effect on the number of scratches. It reduced serum IL-17 levels (<i>p</i> < 0.01). It alleviated local inflammation by suppressing CD8<sup>+</sup> T lymphocyte and neutrophil infiltration. <i>Conclusions:</i> It was concluded that remote ischemic postconditioning may alleviate the symptoms of allergic contact dermatitis by suppressing CD8<sup>+</sup> T lymphocyte and neutrophil infiltration and reducing IL-17 secretion. |
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| language | English |
| last_indexed | 2024-03-10T21:04:22Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
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| series | Medicina |
| spelling | doaj.art-fcedf0fc13b94f3488461b0ea0e40cb42023-11-19T17:17:40ZengMDPI AGMedicina1010-660X1648-91442023-10-015910181610.3390/medicina59101816Anti-Inflammatory and Antipruritic Effects of Remote Ischaemic Postconditioning in a Mouse Model of Experimental Allergic Contact DermatitisOzgur Gunduz0Melike Sapmaz-Metin1Ruhan Deniz Topuz2Oktay Kaya3Cetin Hakan Karadag4Ahmet Ulugol5Department of Medical Pharmacology, Faculty of Medicine, Trakya University, 22030 Edirne, TurkeyDepartment of Histology and Embryology, Faculty of Medicine, Trakya University, 22030 Edirne, TurkeyDepartment of Medical Pharmacology, Faculty of Medicine, Trakya University, 22030 Edirne, TurkeyDepartment of Physiology, Faculty of Medicine, Trakya University, 22030 Edirne, TurkeyDepartment of Medical Pharmacology, Faculty of Medicine, Trakya University, 22030 Edirne, TurkeyDepartment of Medical Pharmacology, Faculty of Medicine, Trakya University, 22030 Edirne, Turkey<i>Background and Objectives:</i> Allergic contact dermatitis is a common type IV hypersensitivity reaction characterised by redness, itching, oedema and thickening of the skin. It occurs in about 7% of the population and its incidence is increasing. It has been observed that the preconditioning of tissues by exposing them to transient ischemia increases resistance to subsequent permanent ischemia, and this phenomenon is called ischemic preconditioning. It has been shown that conditioning in one organ can also protect other organs. The protective effect of remote ischemic preconditioning is thought to be based on the induction of anti-inflammatory responses. The aim of this project was to investigate the anti-inflammatory and antipruritic effects of remote ischemic postconditioning in a mouse model of experimental allergic contact dermatitis. <i>Methods:</i> Experimental allergic contact dermatitis was induced with 1-fluoro-2,4-dinitrobenzene. Remote ischemic postconditioning was performed at 3 and 25 h after the challenge. Ear thickness and number of scratches 24 and 48 h after challenge, as well as cytokine levels and the infiltration of mast cells, neutrophils, CD4<sup>+</sup> and CD8<sup>+</sup> T lymphocytes in serum and ear tissue at 48 h were measured to determine the effect of RIPsC. <i>Results:</i> Remote ischemic postconditioning decreased ear thickness, one of the symptoms of allergic contact dermatitis (<i>p</i> < 0.0001). It had no significant effect on the number of scratches. It reduced serum IL-17 levels (<i>p</i> < 0.01). It alleviated local inflammation by suppressing CD8<sup>+</sup> T lymphocyte and neutrophil infiltration. <i>Conclusions:</i> It was concluded that remote ischemic postconditioning may alleviate the symptoms of allergic contact dermatitis by suppressing CD8<sup>+</sup> T lymphocyte and neutrophil infiltration and reducing IL-17 secretion.https://www.mdpi.com/1648-9144/59/10/1816allergic contact dermatitisremote ischemic postconditioninginflammationpruritusscratching behavior |
| spellingShingle | Ozgur Gunduz Melike Sapmaz-Metin Ruhan Deniz Topuz Oktay Kaya Cetin Hakan Karadag Ahmet Ulugol Anti-Inflammatory and Antipruritic Effects of Remote Ischaemic Postconditioning in a Mouse Model of Experimental Allergic Contact Dermatitis Medicina allergic contact dermatitis remote ischemic postconditioning inflammation pruritus scratching behavior |
| title | Anti-Inflammatory and Antipruritic Effects of Remote Ischaemic Postconditioning in a Mouse Model of Experimental Allergic Contact Dermatitis |
| title_full | Anti-Inflammatory and Antipruritic Effects of Remote Ischaemic Postconditioning in a Mouse Model of Experimental Allergic Contact Dermatitis |
| title_fullStr | Anti-Inflammatory and Antipruritic Effects of Remote Ischaemic Postconditioning in a Mouse Model of Experimental Allergic Contact Dermatitis |
| title_full_unstemmed | Anti-Inflammatory and Antipruritic Effects of Remote Ischaemic Postconditioning in a Mouse Model of Experimental Allergic Contact Dermatitis |
| title_short | Anti-Inflammatory and Antipruritic Effects of Remote Ischaemic Postconditioning in a Mouse Model of Experimental Allergic Contact Dermatitis |
| title_sort | anti inflammatory and antipruritic effects of remote ischaemic postconditioning in a mouse model of experimental allergic contact dermatitis |
| topic | allergic contact dermatitis remote ischemic postconditioning inflammation pruritus scratching behavior |
| url | https://www.mdpi.com/1648-9144/59/10/1816 |
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