Negative Correlation between Lipid Content and Antibiotic Activity in <i>Streptomyces</i>: General Rule and Exceptions
Streptomycetes are well known antibiotic producers and are among the rare prokaryotes able to store carbon as lipids. Previous comparative studies of the weak antibiotic producer <i>Streptomyces lividans</i> with its <i>ppk</i> mutant and with <i>Streptomyces coelicolor...
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| Format: | Article |
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MDPI AG
2020-05-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/9/6/280 |
| _version_ | 1797567056666689536 |
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| author | Michelle David Clara Lejeune Sonia Abreu Annabelle Thibessard Pierre Leblond Pierre Chaminade Marie-Joelle Virolle |
| author_facet | Michelle David Clara Lejeune Sonia Abreu Annabelle Thibessard Pierre Leblond Pierre Chaminade Marie-Joelle Virolle |
| author_sort | Michelle David |
| collection | DOAJ |
| description | Streptomycetes are well known antibiotic producers and are among the rare prokaryotes able to store carbon as lipids. Previous comparative studies of the weak antibiotic producer <i>Streptomyces lividans</i> with its <i>ppk</i> mutant and with <i>Streptomyces coelicolor</i>, which both produce antibiotics, suggested the existence of a negative correlation between total lipid content and the ability to produce antibiotics. To determine whether such a negative correlation can be generalized to other <i>Streptomyces</i> species, fifty-four strains were picked randomly and grown on modified R2YE medium, limited in phosphate, with glucose or glycerol as the main carbon source. The total lipid content and antibiotic activity against <i>Micrococcus luteus</i> were assessed for each strain. This study revealed that the ability to accumulate lipids was not evenly distributed among strains and that glycerol was more lipogenic than glucose and had a negative impact on antibiotic biosynthesis. Furthermore, a statistically significant negative Pearson correlation between lipid content and antibiotic activity could be established for most strains, but a few strains escape this general law. These exceptions are likely due to limits and biases linked to the type of test used to determine antibiotic activity, which relies exclusively on <i>Micrococcus luteus</i> sensitivity. They are characterized either by high lipid content and high antibiotic activity or by low lipid content and undetectable antibiotic activity against <i>Micrococcus luteus</i>. Lastly, the comparative genomic analysis of two strains with contrasting lipid content, and both named <i>Streptomyces antibioticus</i> (DSM 41,481 and DSM 40,868, which we found to be phylogenetically related to <i>Streptomyces lavenduligriseus</i>)<i>,</i> indicated that some genetic differences in various pathways related to the generation/consumption of acetylCoA could be responsible for such a difference. |
| first_indexed | 2024-03-10T19:36:13Z |
| format | Article |
| id | doaj.art-fcefc982a1cf4f9196c52d2f72687535 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T19:36:13Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-fcefc982a1cf4f9196c52d2f726875352023-11-20T01:43:53ZengMDPI AGAntibiotics2079-63822020-05-019628010.3390/antibiotics9060280Negative Correlation between Lipid Content and Antibiotic Activity in <i>Streptomyces</i>: General Rule and ExceptionsMichelle David0Clara Lejeune1Sonia Abreu2Annabelle Thibessard3Pierre Leblond4Pierre Chaminade5Marie-Joelle Virolle6Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Saclay, 91198 Gif-sur-Yvette, FranceInstitute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Saclay, 91198 Gif-sur-Yvette, FranceLipides, Systèmes Analytiques et Biologiques, Université Paris-Saclay, 92296 Châtenay-Malabry, FranceUniversité de Lorraine, INRAE, DynAMic, F-54000 Nancy, FranceUniversité de Lorraine, INRAE, DynAMic, F-54000 Nancy, FranceLipides, Systèmes Analytiques et Biologiques, Université Paris-Saclay, 92296 Châtenay-Malabry, FranceInstitute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Université Paris-Saclay, 91198 Gif-sur-Yvette, FranceStreptomycetes are well known antibiotic producers and are among the rare prokaryotes able to store carbon as lipids. Previous comparative studies of the weak antibiotic producer <i>Streptomyces lividans</i> with its <i>ppk</i> mutant and with <i>Streptomyces coelicolor</i>, which both produce antibiotics, suggested the existence of a negative correlation between total lipid content and the ability to produce antibiotics. To determine whether such a negative correlation can be generalized to other <i>Streptomyces</i> species, fifty-four strains were picked randomly and grown on modified R2YE medium, limited in phosphate, with glucose or glycerol as the main carbon source. The total lipid content and antibiotic activity against <i>Micrococcus luteus</i> were assessed for each strain. This study revealed that the ability to accumulate lipids was not evenly distributed among strains and that glycerol was more lipogenic than glucose and had a negative impact on antibiotic biosynthesis. Furthermore, a statistically significant negative Pearson correlation between lipid content and antibiotic activity could be established for most strains, but a few strains escape this general law. These exceptions are likely due to limits and biases linked to the type of test used to determine antibiotic activity, which relies exclusively on <i>Micrococcus luteus</i> sensitivity. They are characterized either by high lipid content and high antibiotic activity or by low lipid content and undetectable antibiotic activity against <i>Micrococcus luteus</i>. Lastly, the comparative genomic analysis of two strains with contrasting lipid content, and both named <i>Streptomyces antibioticus</i> (DSM 41,481 and DSM 40,868, which we found to be phylogenetically related to <i>Streptomyces lavenduligriseus</i>)<i>,</i> indicated that some genetic differences in various pathways related to the generation/consumption of acetylCoA could be responsible for such a difference.https://www.mdpi.com/2079-6382/9/6/280<i>Streptomyces</i>antibioticslipidstriacylglycerolmetabolismglycerol |
| spellingShingle | Michelle David Clara Lejeune Sonia Abreu Annabelle Thibessard Pierre Leblond Pierre Chaminade Marie-Joelle Virolle Negative Correlation between Lipid Content and Antibiotic Activity in <i>Streptomyces</i>: General Rule and Exceptions Antibiotics <i>Streptomyces</i> antibiotics lipids triacylglycerol metabolism glycerol |
| title | Negative Correlation between Lipid Content and Antibiotic Activity in <i>Streptomyces</i>: General Rule and Exceptions |
| title_full | Negative Correlation between Lipid Content and Antibiotic Activity in <i>Streptomyces</i>: General Rule and Exceptions |
| title_fullStr | Negative Correlation between Lipid Content and Antibiotic Activity in <i>Streptomyces</i>: General Rule and Exceptions |
| title_full_unstemmed | Negative Correlation between Lipid Content and Antibiotic Activity in <i>Streptomyces</i>: General Rule and Exceptions |
| title_short | Negative Correlation between Lipid Content and Antibiotic Activity in <i>Streptomyces</i>: General Rule and Exceptions |
| title_sort | negative correlation between lipid content and antibiotic activity in i streptomyces i general rule and exceptions |
| topic | <i>Streptomyces</i> antibiotics lipids triacylglycerol metabolism glycerol |
| url | https://www.mdpi.com/2079-6382/9/6/280 |
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