Assessment of the Cholesterol-Lowering Effect of MOMAST<sup>®</sup>: Biochemical and Cellular Studies
MOMAST<sup>®</sup> is a patented phenolic complex derived from the olive oil vegetation water, a by-product of the olive oil supply chain, in which hydroxytyrosol (OH-Tyr) and tyrosol (Tyr) and verbascoside are the main compounds. This study was aimed at investigating its hypocholesterol...
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MDPI AG
2022-01-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/14/3/493 |
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| author | Martina Bartolomei Carlotta Bollati Jianqiang Li Anna Arnoldi Carmen Lammi |
| author_facet | Martina Bartolomei Carlotta Bollati Jianqiang Li Anna Arnoldi Carmen Lammi |
| author_sort | Martina Bartolomei |
| collection | DOAJ |
| description | MOMAST<sup>®</sup> is a patented phenolic complex derived from the olive oil vegetation water, a by-product of the olive oil supply chain, in which hydroxytyrosol (OH-Tyr) and tyrosol (Tyr) and verbascoside are the main compounds. This study was aimed at investigating its hypocholesterolemic effect by assessing the ability to modulate the low-density lipoprotein (LDL) receptor (LDLR)/sterol regulatory element-binding protein 2 (SREBP-2), and proprotein convertase subtilisin/kexin type 9 (PCSK9) pathways. MOMAST<sup>®</sup> inhibits the in vitro activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGC<sub>O</sub>AR) with a dose-response trend. After the treatment of HepG2 cells, MOMAST<sup>®</sup> increases the SREBP-2, LDLR, and HMGCoAR protein levels leading, from a functional point of view to an improved ability of hepatic cells to up-take LDL from the extracellular environment with a final cholesterol-lowering effect. Furthermore, MOMAST<sup>®</sup> decreased the PCSK9 protein levels and its secretion in the extracellular environment, presumably via the reduction of the hepatic nuclear factor 1-α (HNF1-α). The experiments were performed in parallel, using pravastatin as a reference compound. Results demonstrated that MOMAST<sup>®</sup> may be exploited as a new ingredient for the development of functional foods and/or nutraceuticals for cardiovascular disease prevention. |
| first_indexed | 2024-03-09T23:23:01Z |
| format | Article |
| id | doaj.art-fcf2c52a4eff42ed8c0c9e05e19855b8 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-09T23:23:01Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-fcf2c52a4eff42ed8c0c9e05e19855b82023-11-23T17:24:41ZengMDPI AGNutrients2072-66432022-01-0114349310.3390/nu14030493Assessment of the Cholesterol-Lowering Effect of MOMAST<sup>®</sup>: Biochemical and Cellular StudiesMartina Bartolomei0Carlotta Bollati1Jianqiang Li2Anna Arnoldi3Carmen Lammi4Department of Pharmaceutical Sciences, University of Milan, Via Mangiagalli 25, 20133 Milan, ItalyDepartment of Pharmaceutical Sciences, University of Milan, Via Mangiagalli 25, 20133 Milan, ItalyDepartment of Pharmaceutical Sciences, University of Milan, Via Mangiagalli 25, 20133 Milan, ItalyDepartment of Pharmaceutical Sciences, University of Milan, Via Mangiagalli 25, 20133 Milan, ItalyDepartment of Pharmaceutical Sciences, University of Milan, Via Mangiagalli 25, 20133 Milan, ItalyMOMAST<sup>®</sup> is a patented phenolic complex derived from the olive oil vegetation water, a by-product of the olive oil supply chain, in which hydroxytyrosol (OH-Tyr) and tyrosol (Tyr) and verbascoside are the main compounds. This study was aimed at investigating its hypocholesterolemic effect by assessing the ability to modulate the low-density lipoprotein (LDL) receptor (LDLR)/sterol regulatory element-binding protein 2 (SREBP-2), and proprotein convertase subtilisin/kexin type 9 (PCSK9) pathways. MOMAST<sup>®</sup> inhibits the in vitro activity of 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGC<sub>O</sub>AR) with a dose-response trend. After the treatment of HepG2 cells, MOMAST<sup>®</sup> increases the SREBP-2, LDLR, and HMGCoAR protein levels leading, from a functional point of view to an improved ability of hepatic cells to up-take LDL from the extracellular environment with a final cholesterol-lowering effect. Furthermore, MOMAST<sup>®</sup> decreased the PCSK9 protein levels and its secretion in the extracellular environment, presumably via the reduction of the hepatic nuclear factor 1-α (HNF1-α). The experiments were performed in parallel, using pravastatin as a reference compound. Results demonstrated that MOMAST<sup>®</sup> may be exploited as a new ingredient for the development of functional foods and/or nutraceuticals for cardiovascular disease prevention.https://www.mdpi.com/2072-6643/14/3/493<i>Cultivar</i> Coratinaby-productscircular economycholesterol metabolismPCSK9 |
| spellingShingle | Martina Bartolomei Carlotta Bollati Jianqiang Li Anna Arnoldi Carmen Lammi Assessment of the Cholesterol-Lowering Effect of MOMAST<sup>®</sup>: Biochemical and Cellular Studies Nutrients <i>Cultivar</i> Coratina by-products circular economy cholesterol metabolism PCSK9 |
| title | Assessment of the Cholesterol-Lowering Effect of MOMAST<sup>®</sup>: Biochemical and Cellular Studies |
| title_full | Assessment of the Cholesterol-Lowering Effect of MOMAST<sup>®</sup>: Biochemical and Cellular Studies |
| title_fullStr | Assessment of the Cholesterol-Lowering Effect of MOMAST<sup>®</sup>: Biochemical and Cellular Studies |
| title_full_unstemmed | Assessment of the Cholesterol-Lowering Effect of MOMAST<sup>®</sup>: Biochemical and Cellular Studies |
| title_short | Assessment of the Cholesterol-Lowering Effect of MOMAST<sup>®</sup>: Biochemical and Cellular Studies |
| title_sort | assessment of the cholesterol lowering effect of momast sup r sup biochemical and cellular studies |
| topic | <i>Cultivar</i> Coratina by-products circular economy cholesterol metabolism PCSK9 |
| url | https://www.mdpi.com/2072-6643/14/3/493 |
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