Design Considerations for Murine Retinal Imaging Using Scattering Angle Resolved Optical Coherence Tomography
Optical coherence tomography (OCT), an optical imaging approach enabling cross-sectional analysis of turbid samples, is routinely used for retinal imaging in human and animal models of diseases affecting the retina. Scattering angle resolved (SAR-)OCT has previously been demonstrated as offering add...
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| Format: | Article |
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MDPI AG
2018-11-01
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| Series: | Applied Sciences |
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| Online Access: | https://www.mdpi.com/2076-3417/8/11/2159 |
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| author | Michael R. Gardner Nitesh Katta Ayesha S. Rahman Henry G. Rylander Thomas E. Milner |
| author_facet | Michael R. Gardner Nitesh Katta Ayesha S. Rahman Henry G. Rylander Thomas E. Milner |
| author_sort | Michael R. Gardner |
| collection | DOAJ |
| description | Optical coherence tomography (OCT), an optical imaging approach enabling cross-sectional analysis of turbid samples, is routinely used for retinal imaging in human and animal models of diseases affecting the retina. Scattering angle resolved (SAR-)OCT has previously been demonstrated as offering additional contrast in human studies, but no SAR-OCT system has been reported in detail for imaging the retinas of mice. An optical model of a mouse eye was designed and extended for validity at wavelengths of light around 1310 nm; this model was then utilized to develop a SAR-OCT design for murine retinal imaging. A Monte Carlo technique simulates light scattering from the retina, and the simulation results are confirmed with SAR-OCT images. Various images from the SAR-OCT system are presented and utility of the system is described. SAR-OCT is demonstrated as a viable and robust imaging platform to extend utility of retinal OCT imaging by incorporating scattering data into investigative ophthalmologic analysis. |
| first_indexed | 2024-12-19T04:11:27Z |
| format | Article |
| id | doaj.art-fcf475ad865e4fd5843e2af82ce65d98 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-12-19T04:11:27Z |
| publishDate | 2018-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-fcf475ad865e4fd5843e2af82ce65d982022-12-21T20:36:24ZengMDPI AGApplied Sciences2076-34172018-11-01811215910.3390/app8112159app8112159Design Considerations for Murine Retinal Imaging Using Scattering Angle Resolved Optical Coherence TomographyMichael R. Gardner0Nitesh Katta1Ayesha S. Rahman2Henry G. Rylander3Thomas E. Milner4Department of Biomedical Engineering, The University of Texas at Austin, 107 W Dean Keeton St, Austin, TX 78712, USADepartment of Biomedical Engineering, The University of Texas at Austin, 107 W Dean Keeton St, Austin, TX 78712, USADepartment of Biomedical Engineering, The University of Texas at Austin, 107 W Dean Keeton St, Austin, TX 78712, USADepartment of Biomedical Engineering, The University of Texas at Austin, 107 W Dean Keeton St, Austin, TX 78712, USADepartment of Biomedical Engineering, The University of Texas at Austin, 107 W Dean Keeton St, Austin, TX 78712, USAOptical coherence tomography (OCT), an optical imaging approach enabling cross-sectional analysis of turbid samples, is routinely used for retinal imaging in human and animal models of diseases affecting the retina. Scattering angle resolved (SAR-)OCT has previously been demonstrated as offering additional contrast in human studies, but no SAR-OCT system has been reported in detail for imaging the retinas of mice. An optical model of a mouse eye was designed and extended for validity at wavelengths of light around 1310 nm; this model was then utilized to develop a SAR-OCT design for murine retinal imaging. A Monte Carlo technique simulates light scattering from the retina, and the simulation results are confirmed with SAR-OCT images. Various images from the SAR-OCT system are presented and utility of the system is described. SAR-OCT is demonstrated as a viable and robust imaging platform to extend utility of retinal OCT imaging by incorporating scattering data into investigative ophthalmologic analysis.https://www.mdpi.com/2076-3417/8/11/2159optical coherence tomographyscatteringangle resolved imagingretinal imagingrodent imaging |
| spellingShingle | Michael R. Gardner Nitesh Katta Ayesha S. Rahman Henry G. Rylander Thomas E. Milner Design Considerations for Murine Retinal Imaging Using Scattering Angle Resolved Optical Coherence Tomography Applied Sciences optical coherence tomography scattering angle resolved imaging retinal imaging rodent imaging |
| title | Design Considerations for Murine Retinal Imaging Using Scattering Angle Resolved Optical Coherence Tomography |
| title_full | Design Considerations for Murine Retinal Imaging Using Scattering Angle Resolved Optical Coherence Tomography |
| title_fullStr | Design Considerations for Murine Retinal Imaging Using Scattering Angle Resolved Optical Coherence Tomography |
| title_full_unstemmed | Design Considerations for Murine Retinal Imaging Using Scattering Angle Resolved Optical Coherence Tomography |
| title_short | Design Considerations for Murine Retinal Imaging Using Scattering Angle Resolved Optical Coherence Tomography |
| title_sort | design considerations for murine retinal imaging using scattering angle resolved optical coherence tomography |
| topic | optical coherence tomography scattering angle resolved imaging retinal imaging rodent imaging |
| url | https://www.mdpi.com/2076-3417/8/11/2159 |
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