Overexpression of Semaphorin-3A and Semaphorin-4D in the Peripheral Blood from Newly Diagnosed Patients with Chronic Lymphocytic Leukemia
Background: Semaphorins play prominent roles in physiological and pathological processes such as vascular development, tumor growth and immune responses. Semaphorins have different roles in various kinds of cancers, but there is no study concerning their expression in the chronic lymphocytic leukemi...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2019-01-01
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| Series: | International Journal of Hematology-Oncology and Stem Cell Research |
| Subjects: | |
| Online Access: | https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/915 |
| _version_ | 1797709580643336192 |
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| author | Somayeh Parsa Sedigheh Sharifzadeh Ahmad Monabati Noorossadat Seyyedi Reza Ranjbaran Mohammad Reza Baghbani Maryam Nemati Abdollah Jafarzadeh |
| author_facet | Somayeh Parsa Sedigheh Sharifzadeh Ahmad Monabati Noorossadat Seyyedi Reza Ranjbaran Mohammad Reza Baghbani Maryam Nemati Abdollah Jafarzadeh |
| author_sort | Somayeh Parsa |
| collection | DOAJ |
| description | Background: Semaphorins play prominent roles in physiological and pathological processes such as vascular development, tumor growth and immune responses. Semaphorins have different roles in various kinds of cancers, but there is no study concerning their expression in the chronic lymphocytic leukemia (CLL). This study aimed to assess the SEMA3A, SEMA4A and SEMA4D expression in patients with CLL.
Materials and Methods: Peripheral blood specimens were collected from 30 newly-diagnosed untreated patients with CLL and 30 healthy subjects as a control group. The SEMA3A, SEMA4A and SEMA4D expression was determined by real-time PCR method.
Results: The fold change expression of SEMA3A and SEMA4D was 7.58 ± 2.66 and 3.20 ± 0.99 in patients with CLL, and was 1.01 ± 0.31 and 1.00 ± 0.27 in healthy subjects, respectively. The CLL patients expressed higher amounts of SEMA3A and SEMA4D in comparison with healthy subjects (P<0.02 and P<0.03, respectively). The fold change expression of SEMA3A in patients with stage II (11.12 ± 5.35) was also higher than patients with stage I (4.49 ± 1.61, P<0.05). No significant difference was also observed in the expression of SEMA4A and SEMA4D between patients with stage I and stage II CLL. In both CLL and control groups, the fold change expression of SEMA3A was higher in men than in women (P<0.03 and P<0.02, respectively).
Conclusion: The results of the study indicated elevated expression of the SEMA3A and SEMA4D in patients with CLL. The SEMA3A expression was influenced by tumor stage and gender of participants. |
| first_indexed | 2024-03-12T06:39:44Z |
| format | Article |
| id | doaj.art-fcf4ab4227594b19a4290fe8613519cf |
| institution | Directory Open Access Journal |
| issn | 2008-2207 |
| language | English |
| last_indexed | 2024-03-12T06:39:44Z |
| publishDate | 2019-01-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | International Journal of Hematology-Oncology and Stem Cell Research |
| spelling | doaj.art-fcf4ab4227594b19a4290fe8613519cf2023-09-03T01:08:45ZengTehran University of Medical SciencesInternational Journal of Hematology-Oncology and Stem Cell Research2008-22072019-01-0113110.18502/ijhoscr.v13i1.322Overexpression of Semaphorin-3A and Semaphorin-4D in the Peripheral Blood from Newly Diagnosed Patients with Chronic Lymphocytic LeukemiaSomayeh Parsa0Sedigheh Sharifzadeh1Ahmad Monabati2Noorossadat Seyyedi3Reza Ranjbaran4Mohammad Reza Baghbani5Maryam Nemati6Abdollah Jafarzadeh7Department of Immunology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, IranDiagnostic Laboratory Science and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, IranHematology Research Center, Shiraz University of Medical Sciences, Shiraz, IranDiagnostic Laboratory Science and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, IranDiagnostic Laboratory Science and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, IranDiagnostic Laboratory Science and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, IranDepartment of Laboratory Sciences, School of Para-Medicine, Kerman University of Medical Sciences, kerman, IranMolecular Medicine Research Center, Research Institute of Basic Medical Sciences, Rafsanjan University of Medical Sciences, Rafsanjan, Iran. AND Department of Immunology, School of Medicine, Kerman University of Medical Sciences, Kerman, IranBackground: Semaphorins play prominent roles in physiological and pathological processes such as vascular development, tumor growth and immune responses. Semaphorins have different roles in various kinds of cancers, but there is no study concerning their expression in the chronic lymphocytic leukemia (CLL). This study aimed to assess the SEMA3A, SEMA4A and SEMA4D expression in patients with CLL. Materials and Methods: Peripheral blood specimens were collected from 30 newly-diagnosed untreated patients with CLL and 30 healthy subjects as a control group. The SEMA3A, SEMA4A and SEMA4D expression was determined by real-time PCR method. Results: The fold change expression of SEMA3A and SEMA4D was 7.58 ± 2.66 and 3.20 ± 0.99 in patients with CLL, and was 1.01 ± 0.31 and 1.00 ± 0.27 in healthy subjects, respectively. The CLL patients expressed higher amounts of SEMA3A and SEMA4D in comparison with healthy subjects (P<0.02 and P<0.03, respectively). The fold change expression of SEMA3A in patients with stage II (11.12 ± 5.35) was also higher than patients with stage I (4.49 ± 1.61, P<0.05). No significant difference was also observed in the expression of SEMA4A and SEMA4D between patients with stage I and stage II CLL. In both CLL and control groups, the fold change expression of SEMA3A was higher in men than in women (P<0.03 and P<0.02, respectively). Conclusion: The results of the study indicated elevated expression of the SEMA3A and SEMA4D in patients with CLL. The SEMA3A expression was influenced by tumor stage and gender of participants.https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/915Chronic lymphocytic leukemia; Semaphorins; SEMA3A; SEMA4A; SEMA4D |
| spellingShingle | Somayeh Parsa Sedigheh Sharifzadeh Ahmad Monabati Noorossadat Seyyedi Reza Ranjbaran Mohammad Reza Baghbani Maryam Nemati Abdollah Jafarzadeh Overexpression of Semaphorin-3A and Semaphorin-4D in the Peripheral Blood from Newly Diagnosed Patients with Chronic Lymphocytic Leukemia International Journal of Hematology-Oncology and Stem Cell Research Chronic lymphocytic leukemia; Semaphorins; SEMA3A; SEMA4A; SEMA4D |
| title | Overexpression of Semaphorin-3A and Semaphorin-4D in the Peripheral Blood from Newly Diagnosed Patients with Chronic Lymphocytic Leukemia |
| title_full | Overexpression of Semaphorin-3A and Semaphorin-4D in the Peripheral Blood from Newly Diagnosed Patients with Chronic Lymphocytic Leukemia |
| title_fullStr | Overexpression of Semaphorin-3A and Semaphorin-4D in the Peripheral Blood from Newly Diagnosed Patients with Chronic Lymphocytic Leukemia |
| title_full_unstemmed | Overexpression of Semaphorin-3A and Semaphorin-4D in the Peripheral Blood from Newly Diagnosed Patients with Chronic Lymphocytic Leukemia |
| title_short | Overexpression of Semaphorin-3A and Semaphorin-4D in the Peripheral Blood from Newly Diagnosed Patients with Chronic Lymphocytic Leukemia |
| title_sort | overexpression of semaphorin 3a and semaphorin 4d in the peripheral blood from newly diagnosed patients with chronic lymphocytic leukemia |
| topic | Chronic lymphocytic leukemia; Semaphorins; SEMA3A; SEMA4A; SEMA4D |
| url | https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/915 |
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