The recurrent pathogenic Pro890Leu substitution in CLTC causes a generalized defect in synaptic transmission in Caenorhabditis elegans
De novo CLTC mutations underlie a spectrum of early-onset neurodevelopmental phenotypes having developmental delay/intellectual disability (ID), epilepsy, and movement disorders (MD) as major clinical features. CLTC encodes the widely expressed heavy polypeptide of clathrin, a major component of the...
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Molecular Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnmol.2023.1170061/full |
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| author | Luca Pannone Valentina Muto Francesca Nardecchia Martina Di Rocco Martina Di Rocco Emilia Marchei Federica Tosato Stefania Petrini Giada Onorato Giada Onorato Enrico Lanza Enrico Lanza Lucia Bertuccini Filippo Manti Viola Folli Viola Folli Serena Galosi Elia Di Schiavi Vincenzo Leuzzi Marco Tartaglia Simone Martinelli |
| author_facet | Luca Pannone Valentina Muto Francesca Nardecchia Martina Di Rocco Martina Di Rocco Emilia Marchei Federica Tosato Stefania Petrini Giada Onorato Giada Onorato Enrico Lanza Enrico Lanza Lucia Bertuccini Filippo Manti Viola Folli Viola Folli Serena Galosi Elia Di Schiavi Vincenzo Leuzzi Marco Tartaglia Simone Martinelli |
| author_sort | Luca Pannone |
| collection | DOAJ |
| description | De novo CLTC mutations underlie a spectrum of early-onset neurodevelopmental phenotypes having developmental delay/intellectual disability (ID), epilepsy, and movement disorders (MD) as major clinical features. CLTC encodes the widely expressed heavy polypeptide of clathrin, a major component of the coated vesicles mediating endocytosis, intracellular trafficking, and synaptic vesicle recycling. The underlying pathogenic mechanism is largely unknown. Here, we assessed the functional impact of the recurrent c.2669C > T (p.P890L) substitution, which is associated with a relatively mild ID/MD phenotype. Primary fibroblasts endogenously expressing the mutated protein show reduced transferrin uptake compared to fibroblast lines obtained from three unrelated healthy donors, suggesting defective clathrin-mediated endocytosis. In vitro studies also reveal a block in cell cycle transition from G0/G1 to the S phase in patient’s cells compared to control cells. To demonstrate the causative role of the p.P890L substitution, the pathogenic missense change was introduced at the orthologous position of the Caenorhabditis elegans gene, chc-1 (p.P892L), via CRISPR/Cas9. The resulting homozygous gene-edited strain displays resistance to aldicarb and hypersensitivity to PTZ, indicating defective release of acetylcholine and GABA by ventral cord motor neurons. Consistently, mutant animals show synaptic vesicle depletion at the sublateral nerve cords, and slightly defective dopamine signaling, highlighting a generalized deficit in synaptic transmission. This defective release of neurotransmitters is associated with their secondary accumulation at the presynaptic membrane. Automated analysis of C. elegans locomotion indicates that chc-1 mutants move slower than their isogenic controls and display defective synaptic plasticity. Phenotypic profiling of chc-1 (+/P892L) heterozygous animals and transgenic overexpression experiments document a mild dominant-negative behavior for the mutant allele. Finally, a more severe phenotype resembling that of chc-1 null mutants is observed in animals harboring the c.3146 T > C substitution (p.L1049P), homologs of the pathogenic c.3140 T > C (p.L1047P) change associated with a severe epileptic phenotype. Overall, our findings provide novel insights into disease mechanisms and genotype–phenotype correlations of CLTC-related disorders. |
| first_indexed | 2024-03-13T08:33:28Z |
| format | Article |
| id | doaj.art-fcf6667d12e3415192a604598d029ea0 |
| institution | Directory Open Access Journal |
| issn | 1662-5099 |
| language | English |
| last_indexed | 2024-03-13T08:33:28Z |
| publishDate | 2023-05-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Molecular Neuroscience |
| spelling | doaj.art-fcf6667d12e3415192a604598d029ea02023-05-31T04:18:37ZengFrontiers Media S.A.Frontiers in Molecular Neuroscience1662-50992023-05-011610.3389/fnmol.2023.11700611170061The recurrent pathogenic Pro890Leu substitution in CLTC causes a generalized defect in synaptic transmission in Caenorhabditis elegansLuca Pannone0Valentina Muto1Francesca Nardecchia2Martina Di Rocco3Martina Di Rocco4Emilia Marchei5Federica Tosato6Stefania Petrini7Giada Onorato8Giada Onorato9Enrico Lanza10Enrico Lanza11Lucia Bertuccini12Filippo Manti13Viola Folli14Viola Folli15Serena Galosi16Elia Di Schiavi17Vincenzo Leuzzi18Marco Tartaglia19Simone Martinelli20Molecular Genetics and Functional Genomics, Ospedale Pediatrico Bambino Gesù, IRCCS, Rome, ItalyMolecular Genetics and Functional Genomics, Ospedale Pediatrico Bambino Gesù, IRCCS, Rome, ItalyDepartment of Human Neuroscience, “Sapienza” University of Rome, Rome, ItalyDepartment of Human Neuroscience, “Sapienza” University of Rome, Rome, ItalyDepartment of Oncology and Molecular Medicine, Istituto Superiore di Sanità, Rome, ItalyNational Centre on Addiction and Doping, Istituto Superiore di Sanità, Rome, ItalyDepartment of Oncology and Molecular Medicine, Istituto Superiore di Sanità, Rome, ItalyConfocal Microscopy Core Facility, Ospedale Pediatrico Bambino Gesù, IRCCS, Rome, ItalyInstitute of Biosciences and Bioresources, National Research Council, Naples, ItalyDepartment of Environmental, Biological and Pharmaceutical Science and Technologies, Università degli Studi della Campania “Luigi Vanvitelli”, Caserta, ItalyCenter for Life Nano Science, Istituto Italiano di Tecnologia, Rome, ItalyD-Tails s.r.l., Rome, Italy0Core Facilities, Istituto Superiore di Sanità, Rome, ItalyDepartment of Human Neuroscience, “Sapienza” University of Rome, Rome, ItalyCenter for Life Nano Science, Istituto Italiano di Tecnologia, Rome, ItalyD-Tails s.r.l., Rome, ItalyDepartment of Human Neuroscience, “Sapienza” University of Rome, Rome, ItalyInstitute of Biosciences and Bioresources, National Research Council, Naples, ItalyDepartment of Human Neuroscience, “Sapienza” University of Rome, Rome, ItalyMolecular Genetics and Functional Genomics, Ospedale Pediatrico Bambino Gesù, IRCCS, Rome, ItalyDepartment of Oncology and Molecular Medicine, Istituto Superiore di Sanità, Rome, ItalyDe novo CLTC mutations underlie a spectrum of early-onset neurodevelopmental phenotypes having developmental delay/intellectual disability (ID), epilepsy, and movement disorders (MD) as major clinical features. CLTC encodes the widely expressed heavy polypeptide of clathrin, a major component of the coated vesicles mediating endocytosis, intracellular trafficking, and synaptic vesicle recycling. The underlying pathogenic mechanism is largely unknown. Here, we assessed the functional impact of the recurrent c.2669C > T (p.P890L) substitution, which is associated with a relatively mild ID/MD phenotype. Primary fibroblasts endogenously expressing the mutated protein show reduced transferrin uptake compared to fibroblast lines obtained from three unrelated healthy donors, suggesting defective clathrin-mediated endocytosis. In vitro studies also reveal a block in cell cycle transition from G0/G1 to the S phase in patient’s cells compared to control cells. To demonstrate the causative role of the p.P890L substitution, the pathogenic missense change was introduced at the orthologous position of the Caenorhabditis elegans gene, chc-1 (p.P892L), via CRISPR/Cas9. The resulting homozygous gene-edited strain displays resistance to aldicarb and hypersensitivity to PTZ, indicating defective release of acetylcholine and GABA by ventral cord motor neurons. Consistently, mutant animals show synaptic vesicle depletion at the sublateral nerve cords, and slightly defective dopamine signaling, highlighting a generalized deficit in synaptic transmission. This defective release of neurotransmitters is associated with their secondary accumulation at the presynaptic membrane. Automated analysis of C. elegans locomotion indicates that chc-1 mutants move slower than their isogenic controls and display defective synaptic plasticity. Phenotypic profiling of chc-1 (+/P892L) heterozygous animals and transgenic overexpression experiments document a mild dominant-negative behavior for the mutant allele. Finally, a more severe phenotype resembling that of chc-1 null mutants is observed in animals harboring the c.3146 T > C substitution (p.L1049P), homologs of the pathogenic c.3140 T > C (p.L1047P) change associated with a severe epileptic phenotype. Overall, our findings provide novel insights into disease mechanisms and genotype–phenotype correlations of CLTC-related disorders.https://www.frontiersin.org/articles/10.3389/fnmol.2023.1170061/fullCLTCclathrinsynaptic vesiclesmotor behaviorCaenorhabditis elegans |
| spellingShingle | Luca Pannone Valentina Muto Francesca Nardecchia Martina Di Rocco Martina Di Rocco Emilia Marchei Federica Tosato Stefania Petrini Giada Onorato Giada Onorato Enrico Lanza Enrico Lanza Lucia Bertuccini Filippo Manti Viola Folli Viola Folli Serena Galosi Elia Di Schiavi Vincenzo Leuzzi Marco Tartaglia Simone Martinelli The recurrent pathogenic Pro890Leu substitution in CLTC causes a generalized defect in synaptic transmission in Caenorhabditis elegans Frontiers in Molecular Neuroscience CLTC clathrin synaptic vesicles motor behavior Caenorhabditis elegans |
| title | The recurrent pathogenic Pro890Leu substitution in CLTC causes a generalized defect in synaptic transmission in Caenorhabditis elegans |
| title_full | The recurrent pathogenic Pro890Leu substitution in CLTC causes a generalized defect in synaptic transmission in Caenorhabditis elegans |
| title_fullStr | The recurrent pathogenic Pro890Leu substitution in CLTC causes a generalized defect in synaptic transmission in Caenorhabditis elegans |
| title_full_unstemmed | The recurrent pathogenic Pro890Leu substitution in CLTC causes a generalized defect in synaptic transmission in Caenorhabditis elegans |
| title_short | The recurrent pathogenic Pro890Leu substitution in CLTC causes a generalized defect in synaptic transmission in Caenorhabditis elegans |
| title_sort | recurrent pathogenic pro890leu substitution in cltc causes a generalized defect in synaptic transmission in caenorhabditis elegans |
| topic | CLTC clathrin synaptic vesicles motor behavior Caenorhabditis elegans |
| url | https://www.frontiersin.org/articles/10.3389/fnmol.2023.1170061/full |
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