Urinary podocyte-associated mRNA profile in various stages of diabetic nephropathy.
BACKGROUND: Podocyte injury and subsequent excretion in urine play a crucial role in the pathogenesis and progression of diabetic nephropathy (DN). Quantification of messenger RNA (mRNA) expression in urinary sediment by real-time PCR is emerging as a noninvasive method of screening DN-associated bi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3105067?pdf=render |
| _version_ | 1818158965197897728 |
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| author | Min Zheng Lin-Li Lv Jie Ni Hai-Feng Ni Qing Li Kun-Ling Ma Bi-Cheng Liu |
| author_facet | Min Zheng Lin-Li Lv Jie Ni Hai-Feng Ni Qing Li Kun-Ling Ma Bi-Cheng Liu |
| author_sort | Min Zheng |
| collection | DOAJ |
| description | BACKGROUND: Podocyte injury and subsequent excretion in urine play a crucial role in the pathogenesis and progression of diabetic nephropathy (DN). Quantification of messenger RNA (mRNA) expression in urinary sediment by real-time PCR is emerging as a noninvasive method of screening DN-associated biomarkers. We hypothesized that the urinary mRNA profile of podocyte-associated molecules may provide important clinical insight into the different stages of diabetic nephropathy. METHODS: DN patients (N = 51) and healthy controls (N = 13) were enrolled in this study. DN patients were divided into a normoalbuminuria group (UAE<30 mg/g, n = 17), a microalbuminuria group (UAE 30∼300 mg/g, n = 15), and a macroalbuminuria group (UAE>300 mg/g, n = 19), according to their urinary albumin excretion (UAE). Relative mRNA abundance of synaptopodin, podocalyxin, CD2-AP, α-actin4, and podocin were quantified, and correlations between target mRNAs and clinical parameters were examined. RESULTS: The urinary mRNA levels of all genes studied were significantly higher in the DN group compared with controls (p<0.05), and mRNA levels increased with DN progression. Urinary mRNA levels of all target genes positively correlated with both UAE and BUN. The expression of podocalyxin, CD2-AP, α-actin4, and podocin mRNA correlated with serum creatinine (r = 0.457, p = 0.001; r = 0.329, p = 0.01; r = 0.286, p = 0.021; r = 0.357, p = 0.006, respectively). Furthermore, podocalyxin mRNA was found to negatively correlate with eGFR (r = -0.349, p = 0.01). CONCLUSION: The urinary mRNA profiles of synaptopodin, podocalyxin, CD2-AP, α-actin4, and podocin were found to increase with the progression of DN, which suggested that quantification of podocyte-associated molecules will be useful biomarkers of DN. |
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| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T15:38:28Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
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| spelling | doaj.art-fcf78d95f588469ab91dd2c45faa31cc2022-12-22T00:59:53ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0165e2043110.1371/journal.pone.0020431Urinary podocyte-associated mRNA profile in various stages of diabetic nephropathy.Min ZhengLin-Li LvJie NiHai-Feng NiQing LiKun-Ling MaBi-Cheng LiuBACKGROUND: Podocyte injury and subsequent excretion in urine play a crucial role in the pathogenesis and progression of diabetic nephropathy (DN). Quantification of messenger RNA (mRNA) expression in urinary sediment by real-time PCR is emerging as a noninvasive method of screening DN-associated biomarkers. We hypothesized that the urinary mRNA profile of podocyte-associated molecules may provide important clinical insight into the different stages of diabetic nephropathy. METHODS: DN patients (N = 51) and healthy controls (N = 13) were enrolled in this study. DN patients were divided into a normoalbuminuria group (UAE<30 mg/g, n = 17), a microalbuminuria group (UAE 30∼300 mg/g, n = 15), and a macroalbuminuria group (UAE>300 mg/g, n = 19), according to their urinary albumin excretion (UAE). Relative mRNA abundance of synaptopodin, podocalyxin, CD2-AP, α-actin4, and podocin were quantified, and correlations between target mRNAs and clinical parameters were examined. RESULTS: The urinary mRNA levels of all genes studied were significantly higher in the DN group compared with controls (p<0.05), and mRNA levels increased with DN progression. Urinary mRNA levels of all target genes positively correlated with both UAE and BUN. The expression of podocalyxin, CD2-AP, α-actin4, and podocin mRNA correlated with serum creatinine (r = 0.457, p = 0.001; r = 0.329, p = 0.01; r = 0.286, p = 0.021; r = 0.357, p = 0.006, respectively). Furthermore, podocalyxin mRNA was found to negatively correlate with eGFR (r = -0.349, p = 0.01). CONCLUSION: The urinary mRNA profiles of synaptopodin, podocalyxin, CD2-AP, α-actin4, and podocin were found to increase with the progression of DN, which suggested that quantification of podocyte-associated molecules will be useful biomarkers of DN.http://europepmc.org/articles/PMC3105067?pdf=render |
| spellingShingle | Min Zheng Lin-Li Lv Jie Ni Hai-Feng Ni Qing Li Kun-Ling Ma Bi-Cheng Liu Urinary podocyte-associated mRNA profile in various stages of diabetic nephropathy. PLoS ONE |
| title | Urinary podocyte-associated mRNA profile in various stages of diabetic nephropathy. |
| title_full | Urinary podocyte-associated mRNA profile in various stages of diabetic nephropathy. |
| title_fullStr | Urinary podocyte-associated mRNA profile in various stages of diabetic nephropathy. |
| title_full_unstemmed | Urinary podocyte-associated mRNA profile in various stages of diabetic nephropathy. |
| title_short | Urinary podocyte-associated mRNA profile in various stages of diabetic nephropathy. |
| title_sort | urinary podocyte associated mrna profile in various stages of diabetic nephropathy |
| url | http://europepmc.org/articles/PMC3105067?pdf=render |
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