Proteomic Profiling of Thigh Meat at Different Ages of Chicken for Meat Quality and Development
Chicken age contributes to the meat characteristics; however, knowledge regarding the pathways and proteins associated with meat quality and muscle development are still scarce, especially in chicken thigh meat. Hence, the objective of this study was to elucidate the intricate relationship between t...
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MDPI AG
2023-07-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/12/15/2901 |
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| author | Jian Zhang Xia Chen Jing Cao Cheng Chang Ailian Geng Haihong Wang Qin Chu Zhixun Yan Yao Zhang Huagui Liu |
| author_facet | Jian Zhang Xia Chen Jing Cao Cheng Chang Ailian Geng Haihong Wang Qin Chu Zhixun Yan Yao Zhang Huagui Liu |
| author_sort | Jian Zhang |
| collection | DOAJ |
| description | Chicken age contributes to the meat characteristics; however, knowledge regarding the pathways and proteins associated with meat quality and muscle development are still scarce, especially in chicken thigh meat. Hence, the objective of this study was to elucidate the intricate relationship between these traits by liquid chromatography mass spectrometry at three different ages. A total of 341 differential expressed proteins (DEPs) were screened out (fold change ≥ 1.50 or ≤0.67 and <i>p</i> < 0.05) among 45 thigh meat samples (15 samples per age) of Beijing-You chicken (BYC), collected at the age of 150, 300, or 450 days (D150, D300, and D450), respectively. Subsequently, based on the protein interaction network and Markov cluster algorithm (MCL) analyses, 91 DEPs were divided into 26 MCL clusters, which were associated with pathways of lipid transporter activity, nutrient reservoir activity, signaling pathways of PPAR and MAPK, focal adhesion, ECM-receptor interaction, the cell cycle, oocyte meiosis, ribosomes, taurine and hypotaurine metabolism, glutathione metabolism, muscle contraction, calcium signaling, nucleic acid binding, and spliceosomes. Overall, our data suggest that the thigh meat of BYC at D450 presents the most desirable nutritional value in the term of free amino acids (FAAs) and intramuscular fat (IMF), and a series of proteins and pathways associated with meat quality and development were identified. These findings also provide comprehensive insight regarding these traits across a wide age spectrum. |
| first_indexed | 2024-03-11T00:27:56Z |
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| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-11T00:27:56Z |
| publishDate | 2023-07-01 |
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| series | Foods |
| spelling | doaj.art-fcf8c1ee25d344abb04f47455a6231742023-11-18T22:54:47ZengMDPI AGFoods2304-81582023-07-011215290110.3390/foods12152901Proteomic Profiling of Thigh Meat at Different Ages of Chicken for Meat Quality and DevelopmentJian Zhang0Xia Chen1Jing Cao2Cheng Chang3Ailian Geng4Haihong Wang5Qin Chu6Zhixun Yan7Yao Zhang8Huagui Liu9Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaChicken age contributes to the meat characteristics; however, knowledge regarding the pathways and proteins associated with meat quality and muscle development are still scarce, especially in chicken thigh meat. Hence, the objective of this study was to elucidate the intricate relationship between these traits by liquid chromatography mass spectrometry at three different ages. A total of 341 differential expressed proteins (DEPs) were screened out (fold change ≥ 1.50 or ≤0.67 and <i>p</i> < 0.05) among 45 thigh meat samples (15 samples per age) of Beijing-You chicken (BYC), collected at the age of 150, 300, or 450 days (D150, D300, and D450), respectively. Subsequently, based on the protein interaction network and Markov cluster algorithm (MCL) analyses, 91 DEPs were divided into 26 MCL clusters, which were associated with pathways of lipid transporter activity, nutrient reservoir activity, signaling pathways of PPAR and MAPK, focal adhesion, ECM-receptor interaction, the cell cycle, oocyte meiosis, ribosomes, taurine and hypotaurine metabolism, glutathione metabolism, muscle contraction, calcium signaling, nucleic acid binding, and spliceosomes. Overall, our data suggest that the thigh meat of BYC at D450 presents the most desirable nutritional value in the term of free amino acids (FAAs) and intramuscular fat (IMF), and a series of proteins and pathways associated with meat quality and development were identified. These findings also provide comprehensive insight regarding these traits across a wide age spectrum.https://www.mdpi.com/2304-8158/12/15/2901Beijing-You chickenthigh meatprotein profilefree amino acidintramuscular fat |
| spellingShingle | Jian Zhang Xia Chen Jing Cao Cheng Chang Ailian Geng Haihong Wang Qin Chu Zhixun Yan Yao Zhang Huagui Liu Proteomic Profiling of Thigh Meat at Different Ages of Chicken for Meat Quality and Development Foods Beijing-You chicken thigh meat protein profile free amino acid intramuscular fat |
| title | Proteomic Profiling of Thigh Meat at Different Ages of Chicken for Meat Quality and Development |
| title_full | Proteomic Profiling of Thigh Meat at Different Ages of Chicken for Meat Quality and Development |
| title_fullStr | Proteomic Profiling of Thigh Meat at Different Ages of Chicken for Meat Quality and Development |
| title_full_unstemmed | Proteomic Profiling of Thigh Meat at Different Ages of Chicken for Meat Quality and Development |
| title_short | Proteomic Profiling of Thigh Meat at Different Ages of Chicken for Meat Quality and Development |
| title_sort | proteomic profiling of thigh meat at different ages of chicken for meat quality and development |
| topic | Beijing-You chicken thigh meat protein profile free amino acid intramuscular fat |
| url | https://www.mdpi.com/2304-8158/12/15/2901 |
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