The Study of Antimicrobial Resistance among Salmonella enterica Strains Isolated from Children with Gastroenteritisin in Tehran, Iran
Background: Salmonella enterica is one of the predominant causes of the food-borne salmonellosis in humans. The aim of this study was to determine antimicrobial resistance patterns of Salmonella enterica isolated from stool samples of children with gastroenteritisin in Tehran, Iran. Methods: ...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2020-10-01
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| Series: | Journal of Medical Bacteriology |
| Subjects: | |
| Online Access: | https://jmb.tums.ac.ir/index.php/jmb/article/view/450 |
| _version_ | 1818903316988428288 |
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| author | Fatemeh Fardsanei Mohammad Mehdi Soltan Dallal Mojtaba Memariani Sarah Sharifi-Yazdi Fariba Nabatchian Mohammad Kazem Sharifi-Yazdi |
| author_facet | Fatemeh Fardsanei Mohammad Mehdi Soltan Dallal Mojtaba Memariani Sarah Sharifi-Yazdi Fariba Nabatchian Mohammad Kazem Sharifi-Yazdi |
| author_sort | Fatemeh Fardsanei |
| collection | DOAJ |
| description | Background: Salmonella enterica is one of the predominant causes of the food-borne salmonellosis in humans. The aim of this study was to determine antimicrobial resistance patterns of Salmonella enterica isolated from stool samples of children with gastroenteritisin in Tehran, Iran.
Methods: Stool samples of patients with diarrhea in a pediatric hospital in were collected from June 2017 to May 2018. Microbiological methods were used for identification of Salmonella. The identity of Salmonella enterica serotype enteritidis (S. enteritidis) was also confirmed by a multiplex-PCR. Antibiotic susceptibility testing was performed according to the standard procedure of the Clinical and Laboratory Standards Institute (CLSI).
Results: Of 800 samples, 24 were identified as Salmonella. The most prevalent serotype was S. enteritidis (n=10, 41.7%), followed by S. paratyphi C, (n=6, 25%), S. paratyphi B (n=4, 16.7%), S. arizonae 2 (n=2, 8.3%), and S. paratyphi A (n=2, 8.3%). The highest rates of antibiotic resistance were obtained for nitrofurantoin (100%), followed by nalidixic acid (45.8%), and tetracycline (16.7%). Of 24 S. enteritidis, 9 distinct antibiotypes (Abs) were observed. In this respect, 3 isolates (12.5%) were resistant to at least three or more antibiotics. The most prevalent antibiotype was AB1 (n=8, 33%), which was indicative of resistance to nitrofurantoin.
Conclusion: Considering the constant changes in antibiotic resistance patterns among food-borne pathogens over time, continuous monitoring of multidrug-resistant Salmonella isolates would definitely improve infection control strategies. |
| first_indexed | 2024-12-19T20:49:37Z |
| format | Article |
| id | doaj.art-fcf9f64aa253451ca598896ea715093f |
| institution | Directory Open Access Journal |
| issn | 2251-8649 2322-2581 |
| language | English |
| last_indexed | 2024-12-19T20:49:37Z |
| publishDate | 2020-10-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Journal of Medical Bacteriology |
| spelling | doaj.art-fcf9f64aa253451ca598896ea715093f2022-12-21T20:06:09ZengTehran University of Medical SciencesJournal of Medical Bacteriology2251-86492322-25812020-10-0191-2450The Study of Antimicrobial Resistance among Salmonella enterica Strains Isolated from Children with Gastroenteritisin in Tehran, IranFatemeh Fardsanei0Mohammad Mehdi Soltan Dallal1Mojtaba Memariani2Sarah Sharifi-Yazdi3Fariba Nabatchian4Mohammad Kazem Sharifi-Yazdi5Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Science, Tehran, Iran. AND Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran. Iran.Division of Microbiology, Department of Pathobiology, School of Public Health, Tehran University of Medical Science, Tehran, Iran. AND Food Microbiology Research Center, Tehran University of Medical Sciences, Tehran. Iran.Skin Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.Medical Student, Faculty of Medicine, Tehran University of Medical Sciences, Tehran. Iran.Department of Medical Laboratory Sciences, School of Paramedicine, Tehran University of Medical Sciences. Tehran, Iran.Zoonosis Research Center, Tehran University of Medical Sciences, Tehran, Iran, Department of Medical Laboratory Sciences, School of Paramedicine, Tehran University of Medical Sciences. Tehran, Iran.Background: Salmonella enterica is one of the predominant causes of the food-borne salmonellosis in humans. The aim of this study was to determine antimicrobial resistance patterns of Salmonella enterica isolated from stool samples of children with gastroenteritisin in Tehran, Iran. Methods: Stool samples of patients with diarrhea in a pediatric hospital in were collected from June 2017 to May 2018. Microbiological methods were used for identification of Salmonella. The identity of Salmonella enterica serotype enteritidis (S. enteritidis) was also confirmed by a multiplex-PCR. Antibiotic susceptibility testing was performed according to the standard procedure of the Clinical and Laboratory Standards Institute (CLSI). Results: Of 800 samples, 24 were identified as Salmonella. The most prevalent serotype was S. enteritidis (n=10, 41.7%), followed by S. paratyphi C, (n=6, 25%), S. paratyphi B (n=4, 16.7%), S. arizonae 2 (n=2, 8.3%), and S. paratyphi A (n=2, 8.3%). The highest rates of antibiotic resistance were obtained for nitrofurantoin (100%), followed by nalidixic acid (45.8%), and tetracycline (16.7%). Of 24 S. enteritidis, 9 distinct antibiotypes (Abs) were observed. In this respect, 3 isolates (12.5%) were resistant to at least three or more antibiotics. The most prevalent antibiotype was AB1 (n=8, 33%), which was indicative of resistance to nitrofurantoin. Conclusion: Considering the constant changes in antibiotic resistance patterns among food-borne pathogens over time, continuous monitoring of multidrug-resistant Salmonella isolates would definitely improve infection control strategies.https://jmb.tums.ac.ir/index.php/jmb/article/view/450Antibiotic resistanceAntibiotypesDiarrhea Salmonella enteritidisSalmonellosis. |
| spellingShingle | Fatemeh Fardsanei Mohammad Mehdi Soltan Dallal Mojtaba Memariani Sarah Sharifi-Yazdi Fariba Nabatchian Mohammad Kazem Sharifi-Yazdi The Study of Antimicrobial Resistance among Salmonella enterica Strains Isolated from Children with Gastroenteritisin in Tehran, Iran Journal of Medical Bacteriology Antibiotic resistance Antibiotypes Diarrhea Salmonella enteritidis Salmonellosis. |
| title | The Study of Antimicrobial Resistance among Salmonella enterica Strains Isolated from Children with Gastroenteritisin in Tehran, Iran |
| title_full | The Study of Antimicrobial Resistance among Salmonella enterica Strains Isolated from Children with Gastroenteritisin in Tehran, Iran |
| title_fullStr | The Study of Antimicrobial Resistance among Salmonella enterica Strains Isolated from Children with Gastroenteritisin in Tehran, Iran |
| title_full_unstemmed | The Study of Antimicrobial Resistance among Salmonella enterica Strains Isolated from Children with Gastroenteritisin in Tehran, Iran |
| title_short | The Study of Antimicrobial Resistance among Salmonella enterica Strains Isolated from Children with Gastroenteritisin in Tehran, Iran |
| title_sort | study of antimicrobial resistance among salmonella enterica strains isolated from children with gastroenteritisin in tehran iran |
| topic | Antibiotic resistance Antibiotypes Diarrhea Salmonella enteritidis Salmonellosis. |
| url | https://jmb.tums.ac.ir/index.php/jmb/article/view/450 |
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