| Summary: | Accumulating studies report that microRNAs (miRNAs) are actively involved in skeletal myogenesis. Previously, our study revealed that miR-146b-3p was related to the growth of skeletal muscle. Here, we further report that miR-146b-3p is essential for the proliferation, differentiation, and apoptosis of chicken myoblast. Elevated expression of miR-146b-3p can dramatically suppress proliferation and differentiation, and facilitate apoptosis of chicken myoblast. Besides, we identified two target genes of miR-146b-3p, <i>AKT1</i> and <i>MDFIC</i>, and found that miR-146b-3p can inhibit the <i>PI3K/AKT</i> pathway. Our study also showed that both <i>AKT1</i> and <i>MDFIC</i> can promote the proliferation and differentiation while inhibit the apoptosis of myoblast in chicken. Overall, our results demonstrate that miR-146b-3p, directly suppressing <i>PI3K/AKT</i> pathway and <i>MDFIC</i>, acts in the proliferation, differentiation, and apoptosis of myoblast in chicken.
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