Antibiotic susceptibility of Escherichia coli isolated from neonates admitted to neonatal intensive care units across China from 2015 to 2020
BackgroundEscherichia coli is one of the most common pathogens causing neonatal infections. Recently, the incidence and drug resistance of E. coli have increased, posing a major threat to neonatal health. The aim of this study was to describe and analyze the antibiotic resistance and multilocus sequ...
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Frontiers Media S.A.
2023-05-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1183736/full |
| _version_ | 1797814181356896256 |
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| author | Ruiqi Xiao Ying Li Xiaowei Liu Yijun Ding Jidong Lai Yangfang Li Wenqing Kang Peicen Zou Jie Wang Yue Du Jinjing Zhang Yajuan Wang |
| author_facet | Ruiqi Xiao Ying Li Xiaowei Liu Yijun Ding Jidong Lai Yangfang Li Wenqing Kang Peicen Zou Jie Wang Yue Du Jinjing Zhang Yajuan Wang |
| author_sort | Ruiqi Xiao |
| collection | DOAJ |
| description | BackgroundEscherichia coli is one of the most common pathogens causing neonatal infections. Recently, the incidence and drug resistance of E. coli have increased, posing a major threat to neonatal health. The aim of this study was to describe and analyze the antibiotic resistance and multilocus sequence typing (MLST) characteristics of E. coli derived from infants admitted to neonatal intensive care units (NICUs) across China.MethodsIn this study, 370 strains of E. coli from neonates were collected. E. coli isolated from these specimens were subjected to antimicrobial susceptibility testing (by broth microdilution method) and MLST.ResultsThe overall resistance rate was 82.68%, with the highest rate of methicillin/sulfamethoxazole (55.68%) followed by cefotaxime (46.22%). Multiple resistance rate was 36.74%, 132 strains (35.68%) had extended-spectrum β-lactamase (ESBL) phenotype and 5 strains (1.35%) had insensitivity to the tested carbapenem antibiotics. The resistance of E. coli isolated from different pathogenicity and different sites of infections varied, strains derived from sputum were significantly more resistant to β-lactams and tetracyclines. Currently, the prevalence spectrum in NICUs was dominated by ST1193, ST95, ST73, ST69 and ST131 across China. And the multidrug resistance of ST410 was the most severe. ST410 had the highest resistance rate to cefotaxime (86.67%), and its most common multidrug resistance pattern was β-lactams + aminoglycosides + quinolones + tetracyclines + sulfonamides.ConclusionsSubstantial proportions of neonatal E. coli isolates were severely resistant to commonly administered antibiotics. MLST results can suggest the prevalent characteristics of antibiotic resistance in E. coli with different ST types. |
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| language | English |
| last_indexed | 2024-03-13T08:03:47Z |
| publishDate | 2023-05-01 |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-fcfdd0d849904c588ad926791369add52023-06-01T09:56:25ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882023-05-011310.3389/fcimb.2023.11837361183736Antibiotic susceptibility of Escherichia coli isolated from neonates admitted to neonatal intensive care units across China from 2015 to 2020Ruiqi Xiao0Ying Li1Xiaowei Liu2Yijun Ding3Jidong Lai4Yangfang Li5Wenqing Kang6Peicen Zou7Jie Wang8Yue Du9Jinjing Zhang10Yajuan Wang11Capital Institute of Pediatrics, Beijing, ChinaDepartment of Neonatology, Children’s Hospital, Capital Institute of Pediatrics, Beijing, ChinaBeijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing, ChinaDepartment of Neonatology, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing, ChinaDepartment of Neonatology, Women and Children’s Hospital, School of Medicine, Xiamen University, Xiamen, Fujian, ChinaDepartment of Neonatology, Children’s Hospital of Kunming, Kunming, Yunnan, ChinaNeonatal Intensive Care Unit, Children’s Hospital Affiliated to Zhengzhou University, Henan Children’s Hospital, Zhengzhou, Henan, ChinaCapital Institute of Pediatrics, Beijing, ChinaDepartment of Neonatology, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing, ChinaDepartment of Neonatology, Children’s Hospital, Capital Institute of Pediatrics, Beijing, ChinaDepartment of Neonatology, Beijing Children’s Hospital, Capital Medical University, National Center for Children’s Health, Beijing, ChinaDepartment of Neonatology, Children’s Hospital, Capital Institute of Pediatrics, Beijing, ChinaBackgroundEscherichia coli is one of the most common pathogens causing neonatal infections. Recently, the incidence and drug resistance of E. coli have increased, posing a major threat to neonatal health. The aim of this study was to describe and analyze the antibiotic resistance and multilocus sequence typing (MLST) characteristics of E. coli derived from infants admitted to neonatal intensive care units (NICUs) across China.MethodsIn this study, 370 strains of E. coli from neonates were collected. E. coli isolated from these specimens were subjected to antimicrobial susceptibility testing (by broth microdilution method) and MLST.ResultsThe overall resistance rate was 82.68%, with the highest rate of methicillin/sulfamethoxazole (55.68%) followed by cefotaxime (46.22%). Multiple resistance rate was 36.74%, 132 strains (35.68%) had extended-spectrum β-lactamase (ESBL) phenotype and 5 strains (1.35%) had insensitivity to the tested carbapenem antibiotics. The resistance of E. coli isolated from different pathogenicity and different sites of infections varied, strains derived from sputum were significantly more resistant to β-lactams and tetracyclines. Currently, the prevalence spectrum in NICUs was dominated by ST1193, ST95, ST73, ST69 and ST131 across China. And the multidrug resistance of ST410 was the most severe. ST410 had the highest resistance rate to cefotaxime (86.67%), and its most common multidrug resistance pattern was β-lactams + aminoglycosides + quinolones + tetracyclines + sulfonamides.ConclusionsSubstantial proportions of neonatal E. coli isolates were severely resistant to commonly administered antibiotics. MLST results can suggest the prevalent characteristics of antibiotic resistance in E. coli with different ST types.https://www.frontiersin.org/articles/10.3389/fcimb.2023.1183736/fullneonateneonatal infectionEscherichia coliantimicrobial resistanceMLSTepidemiology |
| spellingShingle | Ruiqi Xiao Ying Li Xiaowei Liu Yijun Ding Jidong Lai Yangfang Li Wenqing Kang Peicen Zou Jie Wang Yue Du Jinjing Zhang Yajuan Wang Antibiotic susceptibility of Escherichia coli isolated from neonates admitted to neonatal intensive care units across China from 2015 to 2020 Frontiers in Cellular and Infection Microbiology neonate neonatal infection Escherichia coli antimicrobial resistance MLST epidemiology |
| title | Antibiotic susceptibility of Escherichia coli isolated from neonates admitted to neonatal intensive care units across China from 2015 to 2020 |
| title_full | Antibiotic susceptibility of Escherichia coli isolated from neonates admitted to neonatal intensive care units across China from 2015 to 2020 |
| title_fullStr | Antibiotic susceptibility of Escherichia coli isolated from neonates admitted to neonatal intensive care units across China from 2015 to 2020 |
| title_full_unstemmed | Antibiotic susceptibility of Escherichia coli isolated from neonates admitted to neonatal intensive care units across China from 2015 to 2020 |
| title_short | Antibiotic susceptibility of Escherichia coli isolated from neonates admitted to neonatal intensive care units across China from 2015 to 2020 |
| title_sort | antibiotic susceptibility of escherichia coli isolated from neonates admitted to neonatal intensive care units across china from 2015 to 2020 |
| topic | neonate neonatal infection Escherichia coli antimicrobial resistance MLST epidemiology |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2023.1183736/full |
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