Construction of induced pluripotent stem cell line (ZZUi0017-A) from the fibroblast cells of a female patient with CACNA1A mutation by unintegrated reprogramming approach
Ataxia is a common clinical symptom of neurodegenerative diseases, such as spinocerebellar ataxia, Parkinson’s disease. Spinocerebellar ataxia includes more than 40 types. In clinical work, we collected the clinical data and skin tissue of one patient with SCA6 who have definitive genetic test resul...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-10-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506120302476 |
| _version_ | 1819003411667877888 |
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| author | Jing Yang Han Liu Huifang Sun Zhuoya Wang Rui Zhang Yutao Liu Qi Zhang Shoutao Zhang Jin Zhang Changhe Shi Yanlin Wang Yuming Xu |
| author_facet | Jing Yang Han Liu Huifang Sun Zhuoya Wang Rui Zhang Yutao Liu Qi Zhang Shoutao Zhang Jin Zhang Changhe Shi Yanlin Wang Yuming Xu |
| author_sort | Jing Yang |
| collection | DOAJ |
| description | Ataxia is a common clinical symptom of neurodegenerative diseases, such as spinocerebellar ataxia, Parkinson’s disease. Spinocerebellar ataxia includes more than 40 types. In clinical work, we collected the clinical data and skin tissue of one patient with SCA6 who have definitive genetic test results. More than that, we reprogrammed the patient derived fibroblast cells to induced pluripotent stem cells (iPSCs) to construct a SCA6 pathological cell model. The cell line was proved having good pluripotency through detection of pluripotent marker and teratoma formation. This iPS cell line is a special cell model for revealing mechanism and identifying potential therapeutic targets. |
| first_indexed | 2024-12-20T23:20:35Z |
| format | Article |
| id | doaj.art-fcfddc03e364403d915051ecb876cc58 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-20T23:20:35Z |
| publishDate | 2020-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-fcfddc03e364403d915051ecb876cc582022-12-21T19:23:33ZengElsevierStem Cell Research1873-50612020-10-0148101946Construction of induced pluripotent stem cell line (ZZUi0017-A) from the fibroblast cells of a female patient with CACNA1A mutation by unintegrated reprogramming approachJing Yang0Han Liu1Huifang Sun2Zhuoya Wang3Rui Zhang4Yutao Liu5Qi Zhang6Shoutao Zhang7Jin Zhang8Changhe Shi9Yanlin Wang10Yuming Xu11Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Zhengzhou University, Zhengzhou, Henan 450001, ChinaDepartment of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Zhengzhou University, Zhengzhou, Henan 450001, ChinaDepartment of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Zhengzhou University, Zhengzhou, Henan 450001, ChinaDepartment of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Zhengzhou University, Zhengzhou, Henan 450001, ChinaDepartment of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Zhengzhou University, Zhengzhou, Henan 450001, ChinaDepartment of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Zhengzhou University, Zhengzhou, Henan 450001, ChinaDepartment of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Zhengzhou University, Zhengzhou, Henan 450001, ChinaSchool of Life Sciences, Zhengzhou University, Zhengzhou, Henan 450001, China; Zhengzhou University, Zhengzhou, Henan 450001, ChinaCenter for Stem Cell and Regenerative Medicine, Department of Basic Medical Sciences, and The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, Zhejiang 310058, China; Institute of Hematology, Zhejiang University, Hangzhou, Zhejiang 310058, China; Zhengzhou University, Zhengzhou, Henan 450001, ChinaDepartment of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Zhengzhou University, Zhengzhou, Henan 450001, China; Corresponding authors at: Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Zhengzhou University, Zhengzhou, Henan 450001, China; Corresponding authors at: Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China; Zhengzhou University, Zhengzhou, Henan 450001, China; Corresponding authors at: Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.Ataxia is a common clinical symptom of neurodegenerative diseases, such as spinocerebellar ataxia, Parkinson’s disease. Spinocerebellar ataxia includes more than 40 types. In clinical work, we collected the clinical data and skin tissue of one patient with SCA6 who have definitive genetic test results. More than that, we reprogrammed the patient derived fibroblast cells to induced pluripotent stem cells (iPSCs) to construct a SCA6 pathological cell model. The cell line was proved having good pluripotency through detection of pluripotent marker and teratoma formation. This iPS cell line is a special cell model for revealing mechanism and identifying potential therapeutic targets.http://www.sciencedirect.com/science/article/pii/S1873506120302476 |
| spellingShingle | Jing Yang Han Liu Huifang Sun Zhuoya Wang Rui Zhang Yutao Liu Qi Zhang Shoutao Zhang Jin Zhang Changhe Shi Yanlin Wang Yuming Xu Construction of induced pluripotent stem cell line (ZZUi0017-A) from the fibroblast cells of a female patient with CACNA1A mutation by unintegrated reprogramming approach Stem Cell Research |
| title | Construction of induced pluripotent stem cell line (ZZUi0017-A) from the fibroblast cells of a female patient with CACNA1A mutation by unintegrated reprogramming approach |
| title_full | Construction of induced pluripotent stem cell line (ZZUi0017-A) from the fibroblast cells of a female patient with CACNA1A mutation by unintegrated reprogramming approach |
| title_fullStr | Construction of induced pluripotent stem cell line (ZZUi0017-A) from the fibroblast cells of a female patient with CACNA1A mutation by unintegrated reprogramming approach |
| title_full_unstemmed | Construction of induced pluripotent stem cell line (ZZUi0017-A) from the fibroblast cells of a female patient with CACNA1A mutation by unintegrated reprogramming approach |
| title_short | Construction of induced pluripotent stem cell line (ZZUi0017-A) from the fibroblast cells of a female patient with CACNA1A mutation by unintegrated reprogramming approach |
| title_sort | construction of induced pluripotent stem cell line zzui0017 a from the fibroblast cells of a female patient with cacna1a mutation by unintegrated reprogramming approach |
| url | http://www.sciencedirect.com/science/article/pii/S1873506120302476 |
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