Maize Apoplastic Fluid Bacteria Alter Feeding Characteristics of Herbivore (<i>Spodoptera frugiperda</i>) in Maize
Maize is an important cereal crop which is severely affected by <i>Spodoptera frugiperda</i>. The study aims to identify endophytic bacteria of maize root and leaf apoplastic fluid with bioprotective traits against <i>S. frugiperda</i> and plant growth promoting properties. A...
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MDPI AG
2022-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/9/1850 |
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| author | Sellappan Ranjith Thangavel Kalaiselvi Muruganagounder Muthusami Uthandi Sivakumar |
| author_facet | Sellappan Ranjith Thangavel Kalaiselvi Muruganagounder Muthusami Uthandi Sivakumar |
| author_sort | Sellappan Ranjith |
| collection | DOAJ |
| description | Maize is an important cereal crop which is severely affected by <i>Spodoptera frugiperda</i>. The study aims to identify endophytic bacteria of maize root and leaf apoplastic fluid with bioprotective traits against <i>S. frugiperda</i> and plant growth promoting properties. Among 15 bacterial endophytic isolates, two strains—namely, RAF5 and LAF5—were selected and identified as <i>Alcaligenes</i> sp. MZ895490 and <i>Bacillus amyloliquefaciens</i> MZ895491, respectively. The bioprotective potential of <i>B. amyloliquefaciens</i> was evaluated through bioassays. In a no-choice bioassay, second instar larvae of <i>S. frugiperda</i> fed on <i>B. amyloliquefaciens</i> treated leaves (B+) recorded comparatively lesser growth (1.10 ± 0.19 mg mg<sup>−1</sup> day<sup>−1</sup>) and consumptive (7.16 ± 3.48 mg mg<sup>−1</sup> day<sup>−1</sup>) rates. In larval dip and choice bioassay, the same trend was observed. In detached leaf experiment, leaf feeding deterrence of <i>S. frugiperda</i> was found to be greater due to inoculation with <i>B</i>. <i>amyloliquefaciens</i> than <i>Alcaligenes</i> sp. The phenolics content of <i>B. amyloliquefaciens</i> inoculated plant was also found to be greater (3.06 ± 0.09 mg gallic acid g<sup>−1</sup>). However, plant biomass production was more in <i>Alcaligenes</i> sp inoculated treatment. The study thus demonstrates the potential utility of <i>Alcaligenes</i> sp. and <i>B. amyloliquefaciens</i> for improving growth and biotic (<i>S. frugiperda</i>) stress tolerance in maize. |
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| language | English |
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| spelling | doaj.art-fd00b2c22a7e4d23b65d4d8bb8a5bf3f2023-11-23T17:54:16ZengMDPI AGMicroorganisms2076-26072022-09-01109185010.3390/microorganisms10091850Maize Apoplastic Fluid Bacteria Alter Feeding Characteristics of Herbivore (<i>Spodoptera frugiperda</i>) in MaizeSellappan Ranjith0Thangavel Kalaiselvi1Muruganagounder Muthusami2Uthandi Sivakumar3Department of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore 641003, IndiaDepartment of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore 641003, IndiaDepartment of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore 641003, IndiaDepartment of Agricultural Microbiology, Tamil Nadu Agricultural University, Coimbatore 641003, IndiaMaize is an important cereal crop which is severely affected by <i>Spodoptera frugiperda</i>. The study aims to identify endophytic bacteria of maize root and leaf apoplastic fluid with bioprotective traits against <i>S. frugiperda</i> and plant growth promoting properties. Among 15 bacterial endophytic isolates, two strains—namely, RAF5 and LAF5—were selected and identified as <i>Alcaligenes</i> sp. MZ895490 and <i>Bacillus amyloliquefaciens</i> MZ895491, respectively. The bioprotective potential of <i>B. amyloliquefaciens</i> was evaluated through bioassays. In a no-choice bioassay, second instar larvae of <i>S. frugiperda</i> fed on <i>B. amyloliquefaciens</i> treated leaves (B+) recorded comparatively lesser growth (1.10 ± 0.19 mg mg<sup>−1</sup> day<sup>−1</sup>) and consumptive (7.16 ± 3.48 mg mg<sup>−1</sup> day<sup>−1</sup>) rates. In larval dip and choice bioassay, the same trend was observed. In detached leaf experiment, leaf feeding deterrence of <i>S. frugiperda</i> was found to be greater due to inoculation with <i>B</i>. <i>amyloliquefaciens</i> than <i>Alcaligenes</i> sp. The phenolics content of <i>B. amyloliquefaciens</i> inoculated plant was also found to be greater (3.06 ± 0.09 mg gallic acid g<sup>−1</sup>). However, plant biomass production was more in <i>Alcaligenes</i> sp inoculated treatment. The study thus demonstrates the potential utility of <i>Alcaligenes</i> sp. and <i>B. amyloliquefaciens</i> for improving growth and biotic (<i>S. frugiperda</i>) stress tolerance in maize.https://www.mdpi.com/2076-2607/10/9/1850maizeapoplastic fluid bacteriafall armywormbioprotectantbioassay |
| spellingShingle | Sellappan Ranjith Thangavel Kalaiselvi Muruganagounder Muthusami Uthandi Sivakumar Maize Apoplastic Fluid Bacteria Alter Feeding Characteristics of Herbivore (<i>Spodoptera frugiperda</i>) in Maize Microorganisms maize apoplastic fluid bacteria fall armyworm bioprotectant bioassay |
| title | Maize Apoplastic Fluid Bacteria Alter Feeding Characteristics of Herbivore (<i>Spodoptera frugiperda</i>) in Maize |
| title_full | Maize Apoplastic Fluid Bacteria Alter Feeding Characteristics of Herbivore (<i>Spodoptera frugiperda</i>) in Maize |
| title_fullStr | Maize Apoplastic Fluid Bacteria Alter Feeding Characteristics of Herbivore (<i>Spodoptera frugiperda</i>) in Maize |
| title_full_unstemmed | Maize Apoplastic Fluid Bacteria Alter Feeding Characteristics of Herbivore (<i>Spodoptera frugiperda</i>) in Maize |
| title_short | Maize Apoplastic Fluid Bacteria Alter Feeding Characteristics of Herbivore (<i>Spodoptera frugiperda</i>) in Maize |
| title_sort | maize apoplastic fluid bacteria alter feeding characteristics of herbivore i spodoptera frugiperda i in maize |
| topic | maize apoplastic fluid bacteria fall armyworm bioprotectant bioassay |
| url | https://www.mdpi.com/2076-2607/10/9/1850 |
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