Protective Effect of Polymethoxyflavones Isolated from <i>Kaempferia parviflora</i> against TNF-α-Induced Human Dermal Fibroblast Damage
Similar to other organs, the skin undergoes a natural aging process. Moreover, constant direct exposure to environmental stresses, including ultraviolet irradiation, causes the signs of skin aging to appear rather early. Reactive oxygen species (ROS) and inflammatory responses accelerate skin damage...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/10/1609 |
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| author | Hung Manh Phung Sullim Lee Sukyung Hong Sojung Lee Kiwon Jung Ki Sung Kang |
| author_facet | Hung Manh Phung Sullim Lee Sukyung Hong Sojung Lee Kiwon Jung Ki Sung Kang |
| author_sort | Hung Manh Phung |
| collection | DOAJ |
| description | Similar to other organs, the skin undergoes a natural aging process. Moreover, constant direct exposure to environmental stresses, including ultraviolet irradiation, causes the signs of skin aging to appear rather early. Reactive oxygen species (ROS) and inflammatory responses accelerate skin damage in extrinsic aging. In this study, we aimed to investigate the skin protective effects of polymethoxyflavones found in <i>Kaempferia parviflora</i> against oxidative stress and inflammation-induced damage in human dermal fibroblasts (HDFs) stimulated by tumor necrosis factor-α (TNF-α). The experimental data identified 5,7,4′ trimethoxyflavone (TMF) as the most potent constituent in preventing TNF-α-induced HDF damage among the tested compounds and it was not only effective in inhibiting matrix metalloproteinase-1 (MMP-1) production but also in stimulating collagen, type I, and alpha 1 (COLIA1) expression. TMF suppressed TNF-α-stimulated generation of ROS and pro-inflammatory mediators, such as cyclooxygenase-2 (COX-2), interleukin (IL)-1β, and IL-6 in HDFs. TMF also inhibited the pathways regulating fibroblast damage, including mitogen-activated protein kinase (MAPK), activator protein 1 (AP-1), and nuclear factor-kappa B (NF-κB). In conclusion, TMF may be a potential agent for preventing skin aging and other dermatological disorders associated with oxidative stress and inflammation. |
| first_indexed | 2024-03-10T06:45:53Z |
| format | Article |
| id | doaj.art-fd00b9897b3843869df60c8cc75d18ef |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T06:45:53Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-fd00b9897b3843869df60c8cc75d18ef2023-11-22T17:16:41ZengMDPI AGAntioxidants2076-39212021-10-011010160910.3390/antiox10101609Protective Effect of Polymethoxyflavones Isolated from <i>Kaempferia parviflora</i> against TNF-α-Induced Human Dermal Fibroblast DamageHung Manh Phung0Sullim Lee1Sukyung Hong2Sojung Lee3Kiwon Jung4Ki Sung Kang5College of Korean Medicine, Gachon University, Seongnam 13120, KoreaDepartment of Life Science, College of Bio-Nano Technology, Gachon University, Seongnam 13120, KoreaCollege of Pharmacy, C.H.A University, Seongnam 13488, KoreaCollege of Pharmacy, C.H.A University, Seongnam 13488, KoreaCollege of Pharmacy, C.H.A University, Seongnam 13488, KoreaCollege of Korean Medicine, Gachon University, Seongnam 13120, KoreaSimilar to other organs, the skin undergoes a natural aging process. Moreover, constant direct exposure to environmental stresses, including ultraviolet irradiation, causes the signs of skin aging to appear rather early. Reactive oxygen species (ROS) and inflammatory responses accelerate skin damage in extrinsic aging. In this study, we aimed to investigate the skin protective effects of polymethoxyflavones found in <i>Kaempferia parviflora</i> against oxidative stress and inflammation-induced damage in human dermal fibroblasts (HDFs) stimulated by tumor necrosis factor-α (TNF-α). The experimental data identified 5,7,4′ trimethoxyflavone (TMF) as the most potent constituent in preventing TNF-α-induced HDF damage among the tested compounds and it was not only effective in inhibiting matrix metalloproteinase-1 (MMP-1) production but also in stimulating collagen, type I, and alpha 1 (COLIA1) expression. TMF suppressed TNF-α-stimulated generation of ROS and pro-inflammatory mediators, such as cyclooxygenase-2 (COX-2), interleukin (IL)-1β, and IL-6 in HDFs. TMF also inhibited the pathways regulating fibroblast damage, including mitogen-activated protein kinase (MAPK), activator protein 1 (AP-1), and nuclear factor-kappa B (NF-κB). In conclusion, TMF may be a potential agent for preventing skin aging and other dermatological disorders associated with oxidative stress and inflammation.https://www.mdpi.com/2076-3921/10/10/1609skin aging<i>Kaempferia parviflora</i>5,7,4′ trimethoxyflavonehuman dermal fibroblaststumor necrosis factor-αreactive oxygen species |
| spellingShingle | Hung Manh Phung Sullim Lee Sukyung Hong Sojung Lee Kiwon Jung Ki Sung Kang Protective Effect of Polymethoxyflavones Isolated from <i>Kaempferia parviflora</i> against TNF-α-Induced Human Dermal Fibroblast Damage Antioxidants skin aging <i>Kaempferia parviflora</i> 5,7,4′ trimethoxyflavone human dermal fibroblasts tumor necrosis factor-α reactive oxygen species |
| title | Protective Effect of Polymethoxyflavones Isolated from <i>Kaempferia parviflora</i> against TNF-α-Induced Human Dermal Fibroblast Damage |
| title_full | Protective Effect of Polymethoxyflavones Isolated from <i>Kaempferia parviflora</i> against TNF-α-Induced Human Dermal Fibroblast Damage |
| title_fullStr | Protective Effect of Polymethoxyflavones Isolated from <i>Kaempferia parviflora</i> against TNF-α-Induced Human Dermal Fibroblast Damage |
| title_full_unstemmed | Protective Effect of Polymethoxyflavones Isolated from <i>Kaempferia parviflora</i> against TNF-α-Induced Human Dermal Fibroblast Damage |
| title_short | Protective Effect of Polymethoxyflavones Isolated from <i>Kaempferia parviflora</i> against TNF-α-Induced Human Dermal Fibroblast Damage |
| title_sort | protective effect of polymethoxyflavones isolated from i kaempferia parviflora i against tnf α induced human dermal fibroblast damage |
| topic | skin aging <i>Kaempferia parviflora</i> 5,7,4′ trimethoxyflavone human dermal fibroblasts tumor necrosis factor-α reactive oxygen species |
| url | https://www.mdpi.com/2076-3921/10/10/1609 |
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