Implication of synaptotagmins 4 and 7 in activity-dependent somatodendritic dopamine release in the ventral midbrain
Dopamine (DA) neurons can release DA not just from axon terminals, but also from their somatodendritic (STD) compartment through a mechanism that is still incompletely understood. Using voltammetry in mouse mesencephalic brain slices, we find that STD DA release has low capacity and shows a calcium...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Royal Society
2022-03-01
|
| Series: | Open Biology |
| Subjects: | |
| Online Access: | https://royalsocietypublishing.org/doi/10.1098/rsob.210339 |
| _version_ | 1827957919486836736 |
|---|---|
| author | Benoît Delignat-Lavaud Charles Ducrot Willemieke Kouwenhoven Nina Feller Louis-Éric Trudeau |
| author_facet | Benoît Delignat-Lavaud Charles Ducrot Willemieke Kouwenhoven Nina Feller Louis-Éric Trudeau |
| author_sort | Benoît Delignat-Lavaud |
| collection | DOAJ |
| description | Dopamine (DA) neurons can release DA not just from axon terminals, but also from their somatodendritic (STD) compartment through a mechanism that is still incompletely understood. Using voltammetry in mouse mesencephalic brain slices, we find that STD DA release has low capacity and shows a calcium sensitivity that is comparable to that of axonal release. We find that the molecular mechanism of STD DA release differs from axonal release with regard to the implication of synaptotagmin (Syt) calcium sensors. While individual constitutive knockout of Syt4 or Syt7 is not sufficient to reduce STD DA release, the removal of both isoforms reduces this release by approximately 50%, leaving axonal release unimpaired. Our work unveils clear differences in the mechanisms of STD and axonal DA release. |
| first_indexed | 2024-04-09T15:28:36Z |
| format | Article |
| id | doaj.art-fd01fc28c5e34f31b99a1f6db6016faa |
| institution | Directory Open Access Journal |
| issn | 2046-2441 |
| language | English |
| last_indexed | 2024-04-09T15:28:36Z |
| publishDate | 2022-03-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Open Biology |
| spelling | doaj.art-fd01fc28c5e34f31b99a1f6db6016faa2023-04-28T11:07:58ZengThe Royal SocietyOpen Biology2046-24412022-03-0112310.1098/rsob.210339Implication of synaptotagmins 4 and 7 in activity-dependent somatodendritic dopamine release in the ventral midbrainBenoît Delignat-Lavaud0Charles Ducrot1Willemieke Kouwenhoven2Nina Feller3Louis-Éric Trudeau4Department of Pharmacology and Physiology, Université de Montréal, QC, Canada H3T 1J4Department of Pharmacology and Physiology, Université de Montréal, QC, Canada H3T 1J4Department of Pharmacology and Physiology, Université de Montréal, QC, Canada H3T 1J4Department of Pharmacology and Physiology, Université de Montréal, QC, Canada H3T 1J4Department of Pharmacology and Physiology, Université de Montréal, QC, Canada H3T 1J4Dopamine (DA) neurons can release DA not just from axon terminals, but also from their somatodendritic (STD) compartment through a mechanism that is still incompletely understood. Using voltammetry in mouse mesencephalic brain slices, we find that STD DA release has low capacity and shows a calcium sensitivity that is comparable to that of axonal release. We find that the molecular mechanism of STD DA release differs from axonal release with regard to the implication of synaptotagmin (Syt) calcium sensors. While individual constitutive knockout of Syt4 or Syt7 is not sufficient to reduce STD DA release, the removal of both isoforms reduces this release by approximately 50%, leaving axonal release unimpaired. Our work unveils clear differences in the mechanisms of STD and axonal DA release.https://royalsocietypublishing.org/doi/10.1098/rsob.210339synaptotagminsomatodendriticdopamineexocytosisneurotransmission |
| spellingShingle | Benoît Delignat-Lavaud Charles Ducrot Willemieke Kouwenhoven Nina Feller Louis-Éric Trudeau Implication of synaptotagmins 4 and 7 in activity-dependent somatodendritic dopamine release in the ventral midbrain Open Biology synaptotagmin somatodendritic dopamine exocytosis neurotransmission |
| title | Implication of synaptotagmins 4 and 7 in activity-dependent somatodendritic dopamine release in the ventral midbrain |
| title_full | Implication of synaptotagmins 4 and 7 in activity-dependent somatodendritic dopamine release in the ventral midbrain |
| title_fullStr | Implication of synaptotagmins 4 and 7 in activity-dependent somatodendritic dopamine release in the ventral midbrain |
| title_full_unstemmed | Implication of synaptotagmins 4 and 7 in activity-dependent somatodendritic dopamine release in the ventral midbrain |
| title_short | Implication of synaptotagmins 4 and 7 in activity-dependent somatodendritic dopamine release in the ventral midbrain |
| title_sort | implication of synaptotagmins 4 and 7 in activity dependent somatodendritic dopamine release in the ventral midbrain |
| topic | synaptotagmin somatodendritic dopamine exocytosis neurotransmission |
| url | https://royalsocietypublishing.org/doi/10.1098/rsob.210339 |
| work_keys_str_mv | AT benoitdelignatlavaud implicationofsynaptotagmins4and7inactivitydependentsomatodendriticdopaminereleaseintheventralmidbrain AT charlesducrot implicationofsynaptotagmins4and7inactivitydependentsomatodendriticdopaminereleaseintheventralmidbrain AT willemiekekouwenhoven implicationofsynaptotagmins4and7inactivitydependentsomatodendriticdopaminereleaseintheventralmidbrain AT ninafeller implicationofsynaptotagmins4and7inactivitydependentsomatodendriticdopaminereleaseintheventralmidbrain AT louiserictrudeau implicationofsynaptotagmins4and7inactivitydependentsomatodendriticdopaminereleaseintheventralmidbrain |