Isolation and Identification of Staphyococci from Raw Milk of Cows Infected with Mastitis

Twenty-six isolates of staphylococci were recovered from of 100 raw milk samples collected from one hundred cows infected with mastitis. For the isolation of bacteria, Mannitol salt agar was used as a selective medium. Staphylococcal species were identified by API-STAPH system. The identified specie...

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Main Author: Safana, A.S. Al-Taan
Format: Article
Language:English
Published: University of Baghdad, College of Veterinary Medicine 2003-06-01
Series:The Iraqi Journal of Veterinary Medicine
Subjects:
Online Access:https://jcovm.uobaghdad.edu.iq/index.php/Iraqijvm/article/view/1107
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author Safana, A.S. Al-Taan
author_facet Safana, A.S. Al-Taan
author_sort Safana, A.S. Al-Taan
collection DOAJ
description Twenty-six isolates of staphylococci were recovered from of 100 raw milk samples collected from one hundred cows infected with mastitis. For the isolation of bacteria, Mannitol salt agar was used as a selective medium. Staphylococcal species were identified by API-STAPH system. The identified species were: - Staphy aureus (13), Staph. epidermidis(9), Staph. haemolyticus(2), Staph. lugdunensis()), and Staph. hominis). Staphylococcus aureus was the most prevalent pathogen among other Staphyloccocal species isolated in this study. Staph. aureus isolates revealed positive results for the defection of capsule structure and for production of some essential enzymes such as coagulase, phosphatase, DNA ase and haemolysin which are highly associated with the virulance of bacteria. Staph. aureus isolates were tested for their sensitivity to the antibiotics and the results were: 13(100%) isolates were sensitive to Gentamycin and Tetracyclene, 11 (84.6%) isolates were sensitive to Oxacillin, Penicillin, Ampicillin, Erythromycin and Cephalexin, where as only 2 (15.4%) isolates were Methicillin Resistant Staph. aureus (MRSA) which showed multi -resistant  towards many antibiotics used in this work.
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spelling doaj.art-fd047e1f80d048fc8601baed1b7ce88f2022-12-21T17:16:12ZengUniversity of Baghdad, College of Veterinary MedicineThe Iraqi Journal of Veterinary Medicine1609-56932410-74092003-06-0127110.30539/ijvm.v27i1.1107Isolation and Identification of Staphyococci from Raw Milk of Cows Infected with MastitisSafana, A.S. Al-Taan0College of Veterinary Medicine,University of BghdadTwenty-six isolates of staphylococci were recovered from of 100 raw milk samples collected from one hundred cows infected with mastitis. For the isolation of bacteria, Mannitol salt agar was used as a selective medium. Staphylococcal species were identified by API-STAPH system. The identified species were: - Staphy aureus (13), Staph. epidermidis(9), Staph. haemolyticus(2), Staph. lugdunensis()), and Staph. hominis). Staphylococcus aureus was the most prevalent pathogen among other Staphyloccocal species isolated in this study. Staph. aureus isolates revealed positive results for the defection of capsule structure and for production of some essential enzymes such as coagulase, phosphatase, DNA ase and haemolysin which are highly associated with the virulance of bacteria. Staph. aureus isolates were tested for their sensitivity to the antibiotics and the results were: 13(100%) isolates were sensitive to Gentamycin and Tetracyclene, 11 (84.6%) isolates were sensitive to Oxacillin, Penicillin, Ampicillin, Erythromycin and Cephalexin, where as only 2 (15.4%) isolates were Methicillin Resistant Staph. aureus (MRSA) which showed multi -resistant  towards many antibiotics used in this work.https://jcovm.uobaghdad.edu.iq/index.php/Iraqijvm/article/view/1107StaphyococciRaw MilkCowsMastitis
spellingShingle Safana, A.S. Al-Taan
Isolation and Identification of Staphyococci from Raw Milk of Cows Infected with Mastitis
The Iraqi Journal of Veterinary Medicine
Staphyococci
Raw Milk
Cows
Mastitis
title Isolation and Identification of Staphyococci from Raw Milk of Cows Infected with Mastitis
title_full Isolation and Identification of Staphyococci from Raw Milk of Cows Infected with Mastitis
title_fullStr Isolation and Identification of Staphyococci from Raw Milk of Cows Infected with Mastitis
title_full_unstemmed Isolation and Identification of Staphyococci from Raw Milk of Cows Infected with Mastitis
title_short Isolation and Identification of Staphyococci from Raw Milk of Cows Infected with Mastitis
title_sort isolation and identification of staphyococci from raw milk of cows infected with mastitis
topic Staphyococci
Raw Milk
Cows
Mastitis
url https://jcovm.uobaghdad.edu.iq/index.php/Iraqijvm/article/view/1107
work_keys_str_mv AT safanaasaltaan isolationandidentificationofstaphyococcifromrawmilkofcowsinfectedwithmastitis