Isolation and Identification of Staphyococci from Raw Milk of Cows Infected with Mastitis
Twenty-six isolates of staphylococci were recovered from of 100 raw milk samples collected from one hundred cows infected with mastitis. For the isolation of bacteria, Mannitol salt agar was used as a selective medium. Staphylococcal species were identified by API-STAPH system. The identified specie...
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Format: | Article |
Language: | English |
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University of Baghdad, College of Veterinary Medicine
2003-06-01
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Series: | The Iraqi Journal of Veterinary Medicine |
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Online Access: | https://jcovm.uobaghdad.edu.iq/index.php/Iraqijvm/article/view/1107 |
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author | Safana, A.S. Al-Taan |
author_facet | Safana, A.S. Al-Taan |
author_sort | Safana, A.S. Al-Taan |
collection | DOAJ |
description | Twenty-six isolates of staphylococci were recovered from of 100 raw milk samples collected from one hundred cows infected with mastitis. For the isolation of bacteria, Mannitol salt agar was used as a selective medium. Staphylococcal species were identified by API-STAPH system. The identified species were: - Staphy aureus (13), Staph. epidermidis(9), Staph. haemolyticus(2), Staph. lugdunensis()), and Staph. hominis). Staphylococcus aureus was the most prevalent pathogen among other Staphyloccocal species isolated in this study. Staph. aureus isolates revealed positive results for the defection of capsule structure and for production of some essential enzymes such as coagulase, phosphatase, DNA ase and haemolysin which are highly associated with the virulance of bacteria. Staph. aureus isolates were tested for their sensitivity to the antibiotics and the results were: 13(100%) isolates were sensitive to Gentamycin and Tetracyclene, 11 (84.6%) isolates were sensitive to Oxacillin, Penicillin, Ampicillin, Erythromycin and Cephalexin, where as only 2 (15.4%) isolates were Methicillin Resistant Staph. aureus (MRSA) which showed multi -resistant
towards many antibiotics used in this work. |
first_indexed | 2024-12-24T04:05:17Z |
format | Article |
id | doaj.art-fd047e1f80d048fc8601baed1b7ce88f |
institution | Directory Open Access Journal |
issn | 1609-5693 2410-7409 |
language | English |
last_indexed | 2024-12-24T04:05:17Z |
publishDate | 2003-06-01 |
publisher | University of Baghdad, College of Veterinary Medicine |
record_format | Article |
series | The Iraqi Journal of Veterinary Medicine |
spelling | doaj.art-fd047e1f80d048fc8601baed1b7ce88f2022-12-21T17:16:12ZengUniversity of Baghdad, College of Veterinary MedicineThe Iraqi Journal of Veterinary Medicine1609-56932410-74092003-06-0127110.30539/ijvm.v27i1.1107Isolation and Identification of Staphyococci from Raw Milk of Cows Infected with MastitisSafana, A.S. Al-Taan0College of Veterinary Medicine,University of BghdadTwenty-six isolates of staphylococci were recovered from of 100 raw milk samples collected from one hundred cows infected with mastitis. For the isolation of bacteria, Mannitol salt agar was used as a selective medium. Staphylococcal species were identified by API-STAPH system. The identified species were: - Staphy aureus (13), Staph. epidermidis(9), Staph. haemolyticus(2), Staph. lugdunensis()), and Staph. hominis). Staphylococcus aureus was the most prevalent pathogen among other Staphyloccocal species isolated in this study. Staph. aureus isolates revealed positive results for the defection of capsule structure and for production of some essential enzymes such as coagulase, phosphatase, DNA ase and haemolysin which are highly associated with the virulance of bacteria. Staph. aureus isolates were tested for their sensitivity to the antibiotics and the results were: 13(100%) isolates were sensitive to Gentamycin and Tetracyclene, 11 (84.6%) isolates were sensitive to Oxacillin, Penicillin, Ampicillin, Erythromycin and Cephalexin, where as only 2 (15.4%) isolates were Methicillin Resistant Staph. aureus (MRSA) which showed multi -resistant towards many antibiotics used in this work.https://jcovm.uobaghdad.edu.iq/index.php/Iraqijvm/article/view/1107StaphyococciRaw MilkCowsMastitis |
spellingShingle | Safana, A.S. Al-Taan Isolation and Identification of Staphyococci from Raw Milk of Cows Infected with Mastitis The Iraqi Journal of Veterinary Medicine Staphyococci Raw Milk Cows Mastitis |
title | Isolation and Identification of Staphyococci from Raw Milk of Cows Infected with Mastitis |
title_full | Isolation and Identification of Staphyococci from Raw Milk of Cows Infected with Mastitis |
title_fullStr | Isolation and Identification of Staphyococci from Raw Milk of Cows Infected with Mastitis |
title_full_unstemmed | Isolation and Identification of Staphyococci from Raw Milk of Cows Infected with Mastitis |
title_short | Isolation and Identification of Staphyococci from Raw Milk of Cows Infected with Mastitis |
title_sort | isolation and identification of staphyococci from raw milk of cows infected with mastitis |
topic | Staphyococci Raw Milk Cows Mastitis |
url | https://jcovm.uobaghdad.edu.iq/index.php/Iraqijvm/article/view/1107 |
work_keys_str_mv | AT safanaasaltaan isolationandidentificationofstaphyococcifromrawmilkofcowsinfectedwithmastitis |