Emerin anchors Msx1 and its protein partners at the nuclear periphery to inhibit myogenesis
Abstract Background Previous studies have shown that in myogenic precursors, the homeoprotein Msx1 and its protein partners, histone methyltransferases and repressive histone marks, tend to be enriched on target myogenic regulatory genes at the nuclear periphery. The nuclear periphery localization o...
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| Format: | Article |
| Language: | English |
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BMC
2019-04-01
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| Series: | Cell & Bioscience |
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| Online Access: | http://link.springer.com/article/10.1186/s13578-019-0296-9 |
| _version_ | 1818255805322887168 |
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| author | Zhangjing Ma Huiyuan Shi Yi Shen Huixia Li Yu Yang Jiange Yang Hui Zhao Gang Wang Jingqiang Wang |
| author_facet | Zhangjing Ma Huiyuan Shi Yi Shen Huixia Li Yu Yang Jiange Yang Hui Zhao Gang Wang Jingqiang Wang |
| author_sort | Zhangjing Ma |
| collection | DOAJ |
| description | Abstract Background Previous studies have shown that in myogenic precursors, the homeoprotein Msx1 and its protein partners, histone methyltransferases and repressive histone marks, tend to be enriched on target myogenic regulatory genes at the nuclear periphery. The nuclear periphery localization of Msx1 and its protein partners is required for Msx1’s function of preventing myogenic precursors from pre-maturation through repressing target myogenic regulatory genes. However, the mechanisms underlying the maintenance of Msx1 and its protein partners’ nuclear periphery localization are unknown. Results We show that an inner nuclear membrane protein, Emerin, performs as an anchor settled at the inner nuclear membrane to keep Msx1 and its protein partners Ezh2, H3K27me3 enriching at the nuclear periphery, and participates in inhibition of myogenesis mediated by Msx1. Msx1 interacts with Emerin both in C2C12 myoblasts and mouse developing limbs, which is the prerequisite for Emerin mediating the precise location of Msx1, Ezh2, and H3K27me3. The deficiency of Emerin in C2C12 myoblasts disturbs the nuclear periphery localization of Msx1, Ezh2, and H3K27me3, directly indicating Emerin functioning as an anchor. Furthermore, Emerin cooperates with Msx1 to repress target myogenic regulatory genes, and assists Msx1 with inhibition of myogenesis. Conclusions Emerin cooperates with Msx1 to inhibit myogenesis through maintaining the nuclear periphery localization of Msx1 and Msx1’s protein partners. |
| first_indexed | 2024-12-12T17:17:42Z |
| format | Article |
| id | doaj.art-fd088363256a40af81aeb91c4d808d1f |
| institution | Directory Open Access Journal |
| issn | 2045-3701 |
| language | English |
| last_indexed | 2024-12-12T17:17:42Z |
| publishDate | 2019-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Cell & Bioscience |
| spelling | doaj.art-fd088363256a40af81aeb91c4d808d1f2022-12-22T00:17:44ZengBMCCell & Bioscience2045-37012019-04-019111310.1186/s13578-019-0296-9Emerin anchors Msx1 and its protein partners at the nuclear periphery to inhibit myogenesisZhangjing Ma0Huiyuan Shi1Yi Shen2Huixia Li3Yu Yang4Jiange Yang5Hui Zhao6Gang Wang7Jingqiang Wang8State Key Laboratory of Genetic Engineering and Collaborative Innovation Center of Genetics and Development, School of Life Sciences and Zhongshan Hospital, Fudan UniversityState Key Laboratory of Genetic Engineering and Collaborative Innovation Center of Genetics and Development, School of Life Sciences and Zhongshan Hospital, Fudan UniversityState Key Laboratory of Genetic Engineering and Collaborative Innovation Center of Genetics and Development, School of Life Sciences and Zhongshan Hospital, Fudan UniversityState Key Laboratory of Genetic Engineering and Collaborative Innovation Center of Genetics and Development, School of Life Sciences and Zhongshan Hospital, Fudan UniversityState Key Laboratory of Genetic Engineering and Collaborative Innovation Center of Genetics and Development, School of Life Sciences and Zhongshan Hospital, Fudan UniversityState Key Laboratory of Genetic Engineering and Collaborative Innovation Center of Genetics and Development, School of Life Sciences and Zhongshan Hospital, Fudan UniversityZhengzhou Revogene IncState Key Laboratory of Genetic Engineering and Collaborative Innovation Center of Genetics and Development, School of Life Sciences and Zhongshan Hospital, Fudan UniversityState Key Laboratory of Genetic Engineering and Collaborative Innovation Center of Genetics and Development, School of Life Sciences and Zhongshan Hospital, Fudan UniversityAbstract Background Previous studies have shown that in myogenic precursors, the homeoprotein Msx1 and its protein partners, histone methyltransferases and repressive histone marks, tend to be enriched on target myogenic regulatory genes at the nuclear periphery. The nuclear periphery localization of Msx1 and its protein partners is required for Msx1’s function of preventing myogenic precursors from pre-maturation through repressing target myogenic regulatory genes. However, the mechanisms underlying the maintenance of Msx1 and its protein partners’ nuclear periphery localization are unknown. Results We show that an inner nuclear membrane protein, Emerin, performs as an anchor settled at the inner nuclear membrane to keep Msx1 and its protein partners Ezh2, H3K27me3 enriching at the nuclear periphery, and participates in inhibition of myogenesis mediated by Msx1. Msx1 interacts with Emerin both in C2C12 myoblasts and mouse developing limbs, which is the prerequisite for Emerin mediating the precise location of Msx1, Ezh2, and H3K27me3. The deficiency of Emerin in C2C12 myoblasts disturbs the nuclear periphery localization of Msx1, Ezh2, and H3K27me3, directly indicating Emerin functioning as an anchor. Furthermore, Emerin cooperates with Msx1 to repress target myogenic regulatory genes, and assists Msx1 with inhibition of myogenesis. Conclusions Emerin cooperates with Msx1 to inhibit myogenesis through maintaining the nuclear periphery localization of Msx1 and Msx1’s protein partners.http://link.springer.com/article/10.1186/s13578-019-0296-9Msx1EmerinEzh2H3K27me3Nuclear-peripheryMyogenesis |
| spellingShingle | Zhangjing Ma Huiyuan Shi Yi Shen Huixia Li Yu Yang Jiange Yang Hui Zhao Gang Wang Jingqiang Wang Emerin anchors Msx1 and its protein partners at the nuclear periphery to inhibit myogenesis Cell & Bioscience Msx1 Emerin Ezh2 H3K27me3 Nuclear-periphery Myogenesis |
| title | Emerin anchors Msx1 and its protein partners at the nuclear periphery to inhibit myogenesis |
| title_full | Emerin anchors Msx1 and its protein partners at the nuclear periphery to inhibit myogenesis |
| title_fullStr | Emerin anchors Msx1 and its protein partners at the nuclear periphery to inhibit myogenesis |
| title_full_unstemmed | Emerin anchors Msx1 and its protein partners at the nuclear periphery to inhibit myogenesis |
| title_short | Emerin anchors Msx1 and its protein partners at the nuclear periphery to inhibit myogenesis |
| title_sort | emerin anchors msx1 and its protein partners at the nuclear periphery to inhibit myogenesis |
| topic | Msx1 Emerin Ezh2 H3K27me3 Nuclear-periphery Myogenesis |
| url | http://link.springer.com/article/10.1186/s13578-019-0296-9 |
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